A5038237815- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Text Readability and Simplification
- Artificial Intelligence in Healthcare and Education
- RNA Research and Splicing
International Institute of Molecular and Cell Biology
2022-2025
In archaea and metazoa, tRNA exons are ligated by the RNA ligases RtcB RTCB, respectively. The metazoan RTCB forms a stable complex with four additional subunits, DDX1, FAM98B, CGI99 ASHWIN. role assembly of these components remain elusive. Furthermore, we lack structural information how substrates recognized 3'-5' ligases. Here, use thiol-based chemical cross-linking to confirm involvement specific residues in binding present cryo-electron microscopy structure purified five-subunit D. rerio...
Abstract Despite the widespread use of mRNA vaccines against COVID-19, little is known about metabolism therapeutic RNAs. Here we nanopore sequencing 1–3 to analyse individual molecules, focusing on their poly(A) tails. We show that Moderna mRNA-1273 vaccine 4 has a tail around 100 nucleotides, followed by an mΨCmΨAG sequence. In cell lines, undergoes rapid degradation initiated removal, CCR4–NOT-mediated deadenylation. However, in medically relevant preclinical models, particularly...
Abstract Abortive infection (Abi) is a bacterial antiphage defense strategy involving suicide of the infected cell. Some Abi pathways involve polymerases that are related to reverse transcriptases. They unique in way they combine ability synthesize DNA template-independent manner with protein priming. Here, we report crystal and cryo-electron microscopy structures two polymerases: AbiK Abi-P2. Both proteins adopt bilobal structure an RT-like domain comprises palm fingers subdomains helical...
Celem artykułu było pokazanie wad ChatGPT – z punktu widzenia nauczycieli polonistów w kontekście możliwości wykorzystania go przez uczniów przy pisaniu wypracowań szkolnych języka polskiego szkole średniej oraz badaniach naukowych. Aby opisać problem wykorzystano ankietę internetową o zasięgu ogólnopolskim czterech losowo wybranych grupach na Facebooku. Ponadto przeprowadzono analizę jakościową tekstów wygenerowanych ChatGPT. Okazało się, iż sztuczna inteligencja poprawnie analizuje badania...