Essential oils (EOs) have been known for a long time, and they are used in several fields such as medicine aromatherapy, well the food pharmaceutical industries. In last decade, EOs also applied to contrast biodeterioration of cultural heritage, representing powerful resource green conservation strategies. this study, an integrated approach based on microscopic observation, vitro culture, molecular investigation was preliminarily employed identify biological systems colonizing wooden...

Biodeterioration is a complex network of interactions between macro/micro-biological systems and organic/inorganic substrates involving physical chemical alterations, strictly related to their metabolic activities. Concerning microbial deterioration, finding correct approach counteract this process often difficult, requiring an understanding the kind alterations use methods that respect artwork human environmental health. Specific conservative remedial are used for aim. They comprise...

The chemical profile of the Thymus vulgaris (Lamiaceae) essential oil (EO) was investigated in order to evaluate its biological properties against microorganisms affecting two Tholu Bommalu, typical Indian leather puppets stored at International Puppets Museum “Antonio Pasqualino” Palermo, Italy. A GC–MS analysis, using both polar and apolar columns, used determine composition oil. aim this study antimicrobial effectiveness Crithmum maritimum oils vapor phase disinfect heritage puppets....

Cultural heritage constitutive materials can provide excellent substrates for microbial colonization, highly influenced by thermo-hygrometric parameters.In cultural heritage-related environments, a detrimental load may be present both on manufacts surface and in the aerosol.In this study, bacterial fungal colonisation has been investigated three Sicilian confined environments (archive, cave hypogea), each with peculiar structures different parameters.Particular attention paid to...

Abstract In recent decades research in the conservation and restoration field has provided sustainable alternatives to traditional procedures for cleaning or controlling microbial colonization of works art. present study, first time novel bioactive molecules extracted from marine invertebrate organisms (Anthozoa) were tested instead chemical compounds removing protein layers as a biocide fungal bacterial colonization. particular, Bioactive Molecules with Protease activity (BMP), acting...

ABSTRACT Several homeobox genes are expressed in the sea urchin embryo but their roles development have yet to be elucidated. Of particular interest homologues of that mouse and Drosophila involved patterning developing central nervous system (CNS). Here, we report cloning an orthopedia (Otp)-related gene from Paracentrotus lividus, PlOtp. Otp is a single copy zygotic presents unique highly restricted expression pattern. Transcripts were first detected at mid-gastrula stage two pairs oral...

The properties of a X.laevis U1B snRNA gene enhancer have been studied by microinjection in Xenopus oocytes. enhancer-like sequence, defined as short DNA stretch that is able to activate transcription an orientation independent manner, interchangeable between different U genes. sequence alone does not, however, efficiently from SV40 pol II promoter but regains its activity when combined with the U-gene specific proximal element. DNase I rotection experiments show can interact specifically...

One of the new ways warehouse pest control is carbon dioxide treatment, which had no residues on target products. In present research, at first, CO

Aromatic plants represent a source of natural products with medicinal properties, and are also utilized in the food pharmaceutical industries. Recently, need for eco-compatible non-toxic products, safe both environment human health, have been proposed sustainable conservation historic–artistic artifacts. In this study, order to counteract microbial colonization (Aspergillus sp., Streptomyces Micrococcus sp.) on wooden artwork surfaces, Thymus vulgaris L. (Lamiaceae) essential oil (EO)...

The increasing use of nanofertilizers in modern agriculture and their impact on crop yield pest management require further research. In this study, the effects nano-Fe, -Zn, -Cu (which are synthesized based nanochelating technology), urea (N) fertilizers antioxidant activities wheat plants (cv. Chamran), green aphid Schizaphis graminum (Rondani) investigated. authors observed highest levels phenolics non-infested nano-Zn-treated (26% higher compared with control). H2O2 infested...

The sea urchin early histone repeating unit contains one copy of each the five genes whose coordinate expression during development is regulated by gene-specific elements. To learn how within a activator can be prevented to communicate with heterologous promoters, we searched for domain boundaries using enhancer blocking assay. We focused on region near 3′ end H2A gene where stage-specific nuclease cleavage sites appear upon silencing genes. demonstrated that DNA fragment 265 bp in length,...

This study is part of a wider conservation project artistic and anthropological finds located in the Grotto Saints (Licodia Eubea, Alia, Sicily), represents an opportunity for  investigating micro-and macro biological systems colonizing this particular environment. It well-known that the  bio-receptivity surfaces strongly related to its constituent materials environmental parameters, whose effects promote establishment specific biotic communities. particularly true for caves, hypogea...

Essential oils have been used for a long time in several fields of interest. Recently, they also applied the conservation Cultural Heritage to contrast biodeterioration replacing most current biocides toxic humans and environment. Inula crithmoides L. (syn. Limbarda (L.) Dumort) is halophyte species distributed along Mediterranean coasts it as an edible vegetable since young leaves or shoots are eaten raw cooked. Several biological properties determined this plant including antimicrobial...

Objects of historic artistic value, conserved in indoor deposits or exhibited inside museum halls, are strongly influenced by the environmental parameters, as temperature, relative humidity and light quality. Environmental parameters directly impact structural integrity constitutive materials promote microbial colonization on artwork surfaces, leading to biodeterioration. In cultural heritage dedicated environments (CHE), load may exist both art works surface aerosol (bioaerosol),...