A5034397544- Heat shock proteins research
- Extracellular vesicles in disease
- Genetics, Aging, and Longevity in Model Organisms
- thermodynamics and calorimetric analyses
- Endoplasmic Reticulum Stress and Disease
- Mesenchymal stem cell research
- Spaceflight effects on biology
- Tissue Engineering and Regenerative Medicine
- RNA Interference and Gene Delivery
- Vanadium and Halogenation Chemistry
- Neonatal Respiratory Health Research
- Marine Biology and Environmental Chemistry
- Protease and Inhibitor Mechanisms
- MicroRNA in disease regulation
- Mercury impact and mitigation studies
- Bone and Dental Protein Studies
- Electrospun Nanofibers in Biomedical Applications
- Reproductive Biology and Fertility
- Developmental Biology and Gene Regulation
- Sulfur Compounds in Biology
- Sperm and Testicular Function
- Analytical Chemistry and Chromatography
- Liver Disease Diagnosis and Treatment
- Protein Structure and Dynamics
- Physiological and biochemical adaptations
University of Palermo
2014-2025
University of Catania
2016-2019
Istituto Euro Mediterraneo di Scienza e Tecnologia
2014-2018
Cell engraftment, survival and integration during transplantation procedures represent the crux of cell-based therapies. Thus, there have been many studies focused on improving cell viability upon implantation. We used severe oxidative stress to select for a mouse mesoangioblast subpopulation in vitro found that this retained self-renewal myogenic differentiation capacities while notably enhancing survival, proliferation migration relative unselected cells. Additionally, cells presented...
Heat shock proteins (HSPs) are induced in response to many injuries including stroke, neurodegenerative disease, epilepsy, and trauma. The overexpression of one HSP particular, Hsp70, serves a protective role several different models nervous system injury, but has also been linked deleterious some diseases. Hsp70 functions as chaperone protects neurons from protein aggregation toxicity (Parkinson Alzheimer polyglutamine diseases, amyotrophic lateral sclerosis), cells apoptosis disease), is...
Some patients experience statin-induced side effects or prefer nutraceutical approaches for the treatment of dyslipidemia. This has led to a search alternative therapeutic dyslipidemia management. In recent studies Citrus bergamia (known as Bergamot) juice was able reduce serum levels lipids. Such benefit may be attributed high amounts flavonoids contained in Bergamot fruit (neoeriocitrin, neohesperidin, naringin). The aim present study fully investigate extract on cardio-metabolic...
In recent decades, the global vanadium (V) industry has been steadily growing, together with interest in potential use of V compounds as therapeutics, leading to release marine environment and making it an emerging pollutant. Since climate change can amplify sensitivity organisms already facing chemical contamination coastal areas, here, for first time, we investigated combined impact warming conditions on development
The study of ecotoxicity induced by vanadium (V) represents an area increasing interest due to the growing use V in both industrial and pharmaceutical areas. This leads its introduction into water environments, marking a developing problem, especially since rising global temperatures appear intensify toxic properties. Cytotoxicological approaches carried out on whole marine embryos represent valid research tool they grow directly contact with pollutants are equipped highly responsive cells...
Heat shock proteins (HSPs), also known as “stress proteins,” are among the highly conserved and immunogenic shared diverse groups of microbial agents mammals [1]. other types stressful stimuli can increase cellular expression HSPs. These have been categorized into different families according to their molecular mass, for example, HSP110, HSP90, HSP70, HSP60, HSP40, HSP20-30, HSP10 [1–3]. For uniformity, guidelines nomenclature various human HSP proposed [4]. Under physiological conditions,...
This study was designed based on the hypothesis that changes in both levels and surface marker expression of extracellular vesicles (EVs) isolated from cerebrospinal fluid (CSF) may be associated with clinical form, disease activity, severity multiple sclerosis (MS). The analyzes were performed subjects affected by MS or other neurological disorders. EVs, which ultracentrifugation CSF samples, characterized flow cytometry. A panel fluorescent antibodies used to identify EV origin: CD4, CCR3,...
WIN55,212-2 (WIN) is a synthetic agonist of cannabinoid receptors that displays promising antitumour properties. The aim this study to demonstrate WIN able block the migratory ability osteosarcoma cells and characterize mechanisms involved. Using wound healing assay zymography, we showed affects cell migration reduces activity metalloproteases MMP2 MMP9. This effect seemed be independent secreted protein acidic rich in cysteine (SPARC), matricellular involved tissue remodeling extracellular...
Heat shock proteins (HSPs) are a well-characterized molecular chaperones protein family, classified into six major families, according to their size. A wide range of tumors have been shown express atypical levels one or more HSPs, suggesting that they could be used as biomarkers. However, the collective role and possible coordination HSP members, well prognostic significance functional implications deregulated expression in breast cancer (BC) poorly investigated. Here, we systematic...
Mouse mesoangioblasts are vessel‐associated progenitor stem cells endowed with the ability of multipotent mesoderm differentiation. Therefore, they represent a promising tool in regeneration injured tissues. Several studies have demonstrated that homing into blood and tissues mainly controlled by cytokines/chemokines other inflammatory factors. However, little is known about molecular mechanisms regulating their to traverse extracellular matrix (ECM). Here, we demonstrate membrane vesicles...
Regenerative medicine aims to repair damaged or missing cells, tissues organs for the treatment of various diseases, poorly managed with conventional drugs and medical procedures. To date there are different approaches obtain these results. Multimodal regenerative methods include transplant healthy organs, tissues, body stimulation activate a self healing response in as well combined use cells bio-degradable scaffold functional tissues. Certainly, stem derived products promising tools due...
In the present study, double immunofluorescence and immunoblot analysis have been used to show that centrosomes, isolated from Paracentrotus lividus sea urchin embryos at first mitotic metaphase, contain constitutive chaperone, heat-shock protein (HSP) 70. More specifically, we demonstrate centrosomes only HSP70-d isoform, which is one of four isoforms identified in P. lividus. We also provide evidence p34(cell division control kinase-2) t complex polypeptide-1 (TCP-1) α, a subunit TCP-1...
Abstract Certain proteins, including fibroblast growth factor‐2 (FGF‐2) and matrix metalloproteinase‐9 (MMP‐9), have proved very effective in increasing the efficacy of mesoangioblast stem cell therapy repairing damaged tissue. We provide first evidence that mouse cells release FGF‐2 MMP‐9 their active form through production membrane vesicles. These vesicles are produced turned over continuously, but stable for some time extracellular milieu. Mesoangioblasts shed even under oxygen tensions...
Metal pharmaceutical residues often represent emerging toxic pollutants of the aquatic environment, as wastewater treatment plants do not sufficiently remove these compounds. Recently, vanadium (V) derivatives have been considered potential therapeutic factors in several diseases, however, only limited information is available about their impact on environments. This study used sea urchin embryos (Paracentrotus lividus) to test V toxicity, it known they are sensitive doses from...
Changes occurring during evolution in the cis-regulatory landscapes of individual members multigene families might impart diversification their spatiotemporal expression and function. The archetypal member echinoid hbox12/pmar1/micro1 family is hbox12-a, a homeobox-containing gene expressed exclusively by dorsal blastomeres, where it governs dorsal/ventral regulatory network embryogenesis sea urchin Paracentrotus lividus. Here we describe inventory genes P. lividus, highlighting that copy...