Recent studies have initiated a paradigm shift in the understanding of function heat shock proteins (HSP). It is now clear that HSP can and do exit mammalian cells, interact with cells immune system, exert immunoregulatory effects. We recently demonstrated exogenously added HSP70 possesses potent cytokine activity, ability to bind high affinity plasma membrane, elicit rapid intracellular Ca²⁺ flux, activate NF-κB, up-regulate expression pro-inflammatory cytokines human monocytes....

Abstract Detergent-soluble membrane vesicles are actively released by human pancreas (Colo−/Colo+) and colon (CX−/CX+) carcinoma sublines, differing in their capacity to present heat shock protein 70 (Hsp70)/Bag-4 on plasma membranes. Floating properties, acetylcholine esterase activity, composition characterized them as exosomes. An enrichment of Rab-4 documented intracellular transport route from early endosomes the membrane. After solubilization, comparable amounts cytosolic proteins,...

Chemotherapy and radiation therapy are standard therapeutic modalities for patients with cancers, could induce various tumor cell death modalities, releasing derived antigens as well danger signals that either be captured triggering antitumor immune response. Historic studies examining tissue cellular responses to have predominantly focused on damage caused proliferating malignant cells leading their death. However, there is increasing evidence also leads significant alterations in the...

Abstract Our study demonstrates that tumor-derived heat shock protein (HSP)70 chaperones a tyrosinase peptide and mediates its transfer to human immature dendritic cells (DCs) by receptor-dependent uptake. Human HSP70 complexes (HSP70-PC) thus have the immunogenic potential instruct DCs cross-present endogenously expressed, nonmutated, tumor antigenic peptides are shared among tumors of melanocytic lineage for T cell recognition. stimulation HSP70-instructed is dependent on Ag bound in only...

Human NK cells (with CD3-/56+ phenotype) acquired features characteristic of apoptosis after incubation with autologous monocytes, as revealed by apoptotic nuclear morphology, degradation DNA into oligonucleosomal fragments, and reduced interchalation propidium iodide. In contrast, T (CD3+/56-) remained non-apoptotic. The monocyte-induced in was prevented catalase, a scavenger hydrogen peroxide; whereas superoxide dismutase (a anion), hydroxyl radical scavengers such mannitol deferoxamine,...

Monocytes, recovered by centrifugal elutriation, effectively inhibit functions of human NK cells in vitro. Histamine, acting via monocyte H2-type histamine receptors, abrogates the inhibitory signal. The aim this study was to define histamine-reversible mechanism which monocytes with special reference respiratory burst activity monocytes. Monocytes from patients chronic granulomatous disease did not suppress cell function, indicating requirement intact nicotinamide-adenine dinucleotide...

Abstract Background Triple-negative breast cancer (TNBC) exhibit characteristics quite distinct from other kinds of cancer, presenting as an aggressive disease--recurring and metastasizing more often than without tumor-specific treatment options accounts for 15% all types with higher percentages in premenopausal African-American Hispanic women. The reason this phenotype is currently the focus intensive research. However, progress hampered by lack suitable TNBC cell model systems. Methods To...

IFN-gamma exhibits differential effects depending on the target and can induce cellular activation enhance survival or mediate cell death via of apoptotic pathways. In this study, we demonstrate an alternative mechanism by which enhances tumor recognition, mediated active release Hsp72. We that stimulation 4T1 breast adenocarcinoma cells K562 erythroleukemic with triggers stress response, results in enhanced expression total Hsp72 without a significant increase death. Intracellular was...

Enteroaggregative Escherichia coli (EAEC) are quite heterogeneous category of an emerging enteric pathogen associated with cases acute or persistent diarrhea worldwide in children and adults, over the past decade has received increasing attention as a cause watery diarrhea, which is often persistent. EAEC infection important outbreak non-outbreak settings developing developed countries. Recently, been implicated development irritable bowel syndrome, but this remains to be confirmed. defined...

Radiotherapy is used to treat approximately 50% of all cancer patients, with varying success. Radiation therapy has become an in­tegral part modern treatment strategies for many types in recent decades, but associated a risk long-term adverse effects. Of these side effects, car­diac complications are particularly relevant since they not only adversely affect quality life can also be potentially life-threat­ening. The dose ionizing radiation that given the tumor determined by sensitivity...

Classic cancer research for several decades has focused on understanding the biology of tumor cells in vitro. However, extending these findings to vivo settings been impeded owing limited insights impact microenvironment cells. We hypothesized that cell and treatment response would be more informative when done presence stromal components, like endothelial cells, which exist microenvironment. To end, we have developed a system grow three-dimensional cultures GFP-4T1 mouse mammary 2H11 murine...

The expression of unique surface structures on tumors that allow for recognition and activation host immunocompetent cells plays an important role in determining tumor growth and/or metastasis. Recent studies have identified heat shock proteins (Hsp) antitumor surveillance; however, the exact Hsp expressed has not been fully addressed. In this study, we show 4T1 mammary adenocarcinoma sorted high Hsp25 (Hsp25high) grow significantly faster than intermediate (Hsp25intermediate) or wild-type...

Abstract Background There is ethnopharmacological evidence that Petiveria alliacea can have antitumor activity; however, the mechanism of its cytotoxic activity not well understood. We assessed multiple in vitro biological activities an ethyl acetate soluble plant fraction over several tumor cell lines. Methods Tumor lines were evaluated using following tests: trypan blue exclusion test, MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide], flow cytometry, cytoskeleton...

Abstract Background Several treatment alternatives are available for primary breast cancer, although those metastatic disease or inflammation associated with tumor progression ineffective. Therefore, there is a great need new therapeutic capable of generating an immune response against residual cells, thus contributing to eradication micrometastases and cancer stem cells. The use complex natural products excellent alternative widely used by Chinese, Hindu, Egyptian, ancestral Latin-American...