A5063322031- Drug-Induced Hepatotoxicity and Protection
- Osteoarthritis Treatment and Mechanisms
- Liver physiology and pathology
- Heat shock proteins research
- Extracellular vesicles in disease
- Liver Disease and Transplantation
- Immune cells in cancer
- Cell Adhesion Molecules Research
- Genetics, Aging, and Longevity in Model Organisms
- Immune Response and Inflammation
- Spaceflight effects on biology
- Knee injuries and reconstruction techniques
- Cancer-related molecular mechanisms research
- Inflammatory mediators and NSAID effects
- Antimicrobial Peptides and Activities
- Chemokine receptors and signaling
- RNA regulation and disease
- Veterinary Equine Medical Research
- interferon and immune responses
- Radiopharmaceutical Chemistry and Applications
- Connective tissue disorders research
- Liver Disease Diagnosis and Treatment
- Shoulder Injury and Treatment
- dental development and anomalies
- Endoplasmic Reticulum Stress and Disease
MRC Centre for Regenerative Medicine
2024-2025
University of Edinburgh
2021-2025
Centre for Inflammation Research
2021
Queen's Medical Centre
2021
Masaryk University
2019
Central European Institute of Technology – Masaryk University
2019
Children's Hospital of Philadelphia
2012-2016
University of Palermo
2003-2010
To study the cellular mechanism of tendon repair process, we used a mouse Achilles injury model to focus on cells recruited injured site. The isolated from 1 week after surgery and uninjured tendons contained connective tissue progenitor populations as determined by colony-forming capacity, cell surface markers, multipotency. When tendon-derived (inTPCs) were transplanted into tendons, they not only integrated in regenerating area expressing tenogenic phenotype but also trans-differentiated...
Abstract Acute liver failure is a rapidly progressing, life-threatening condition most commonly caused by an overdose of acetaminophen (paracetamol). The antidote, N-acetylcysteine (NAC), has limited efficacy when injury established. If acute damage severe, can develop with associated high mortality rates. We have previously demonstrated that alternatively, activated macrophages are potential therapeutic option to reverse in pre-clinical models. In this paper, we present data using...
Slow proliferation is one of the characteristics stem cells. We examined presence, distribution, and regulation slow-cycling cells in developing growing skeleton using a pulse-chase method with new nucleoside derivative, 5-ethynyl-2'-deoxyuridine (EdU). C57BL/6 mice received daily intraperitoneal injections EdU from postnatal day 4 to 7. One after last injection, large population articular cartilage growth plate was labeled. Six weeks number EdU-labeled dramatically decreased, but small them...
Viral and cellular double-stranded RNA (dsRNA) is recognized by cytosolic innate immune sensors, including RIG-I-like receptors. Some cytoplasmic dsRNA commonly present in cells, one source mitochondrial dsRNA, which results from bidirectional transcription of DNA (mtDNA). Here we demonstrate that Trp53 mutant mouse embryonic fibroblasts contain immune-stimulating endogenous origin. We show the response induced this mediated via receptors leads to expression type I interferon proinflammatory...
Osteoarthritis (OA) is one of most common skeletal disorders and can affect synovial joints such as knee ankle joints. α5 integrin, a major fibronectin receptor, expressed in articular cartilage has been demonstrated to play roles joint development the regulation chondrocyte survival matrix degradation cartilage. We hypothesized that integrin signaling involved pathogenesis OA. To test this, we generated compound mice conditionally ablate using Gdf5Cre system. The were born normally had an...
Abstract Certain proteins, including fibroblast growth factor‐2 (FGF‐2) and matrix metalloproteinase‐9 (MMP‐9), have proved very effective in increasing the efficacy of mesoangioblast stem cell therapy repairing damaged tissue. We provide first evidence that mouse cells release FGF‐2 MMP‐9 their active form through production membrane vesicles. These vesicles are produced turned over continuously, but stable for some time extracellular milieu. Mesoangioblasts shed even under oxygen tensions...
Osteochondromas and enchondromas are the most common tumors affecting skeleton. can occur as multiple lesions, such those in patients with hereditary exostoses. Unexpectedly, while studying role of β-catenin cartilage development, we found that its conditional deletion induces ectopic chondroma-like formation mice. Postnatal ablation induced lateral outgrowth growth plate within 2 weeks after ablation. The masses were present flanking periosteum by 5 persisted for more than 6 months These...
Abstract Introduction Acute Liver Failure (ALF) has no effective treatment other than liver transplantation, and is commonly caused by paracetamol overdose. New treatments are needed to treat prevent ALF. Alternatively activated macrophages (AAMs) can promote resolution of necrosis stimulate hepatocyte proliferation. Using AAMs in unscheduled care requires the use an allogeneic product. A clinical trial determine safety tolerability AAMs. Methods analysis single centre, open-label,...
Introduction Acute liver failure (ALF) has no effective treatment other than transplantation and is commonly caused by paracetamol overdose. New treatments are needed to treat prevent ALF. Alternatively-activated macrophages (AAMs) can promote resolution of necrosis stimulate hepatocyte proliferation. Using AAMs in unscheduled care requires the use an allogeneic product. A clinical trial determine safety tolerability AAMs. Methods analysis single-centre, open-label, dose-escalation, phase 1...
Mouse Hsp70 (70 kDa heat shock protein) is preferentially induced by or stress stimuli. We previously found that constitutively expressed in A6 mouse mesoangioblast stem cells, but its possible role these cells and the control of basal transcription remained unexplored. Here we report absence stress, Ku factor able to bind HSE (heat element) consensus sequence vitro, vivo it bound proximal hsp70 promoter. In addition, show constitutive depends on co-operative interaction different factors...
<title>Abstract</title> Acute liver failure (ALF) is a rapidly progressing, life-threatening condition most commonly caused by an overdose of acetaminophen (paracetamol). The antidote, N-acetylcysteine (NAC), has limited efficacy when injury established. If acute damage severe, can develop with associated high mortality rates. We have previously demonstrated that alternatively activated macrophages are potential therapeutic option to reverse in pre-clinical models. In this paper we present...
Abstract Human β-defensin 3 (HBD3), is an anti-microbial host-defence peptide, that can rapidly enter macrophages to modulate TLR4 responses lipopolysaccharide. However, the molecular mechanisms by which HBD3 exerts this anti-inflammatory influence remain unclear. Here, we show mice deleted for orthologue of have increased acute lipopolysaccharide response in vivo . Furthermore, found limited classical activation, and contemporaneously drove expression IL-4. An increase markers alternative a...