A5065702817- Scientific Computing and Data Management
- Plant and animal studies
- Insect and Pesticide Research
- Insect and Arachnid Ecology and Behavior
- Medical Imaging and Pathology Studies
- Inorganic Fluorides and Related Compounds
- Big Data and Business Intelligence
- Cytokine Signaling Pathways and Interactions
- Developmental Biology and Gene Regulation
- Fluorine in Organic Chemistry
- Medical Imaging Techniques and Applications
- Bone health and treatments
- Renal and related cancers
- interferon and immune responses
- Business Process Modeling and Analysis
- Neuroscience and Neuropharmacology Research
- RNA and protein synthesis mechanisms
- Bioinformatics and Genomic Networks
- Liver physiology and pathology
- 3D Printing in Biomedical Research
- Immune cells in cancer
- Genetic and Kidney Cyst Diseases
- Genomics and Chromatin Dynamics
- Protist diversity and phylogeny
- Gene Regulatory Network Analysis
Scottish National Blood Transfusion Service
2024-2025
Roslin Cells (United Kingdom)
2023-2024
Roslin Institute
2012-2023
University of Edinburgh
2003-2023
Columbia University
2023
Chiang Mai University
2023
Royal Ottawa Mental Health Centre
2018
Wellcome Sanger Institute
2006
University of Warwick
1998-2001
This work describes the first genome-wide analysis of transcriptional landscape pig. A new porcine Affymetrix expression array was designed in order to provide comprehensive coverage known pig transcriptome. The used generate a atlas tissues derived from 62 tissue/cell types. These data were subjected network correlation and clustering.The presented here provides detailed functional clustering transcriptome where transcripts are grouped according their pattern, so one can infer function an...
Branching morphogenesis of epithelium is a common and important feature organogenesis; it is, for example, responsible development renal collecting ducts, lung airways, milk ducts mammary glands seminal the prostate. In each case, epithelial controlled by variety mesenchyme-derived molecules, both soluble (e.g. growth factors) insoluble extracellular matrix). Little known about how these varied influences are integrated to produce coherent morphogenetic response, but integration likely be...
Alternative promoter usage and alternative splicing enable diversification of the transcriptome. Here we demonstrate that function Synaptic GTPase-Activating Protein (SynGAP), a key synaptic protein, is determined by combination its amino-terminal sequence with carboxy-terminal sequence. 5′ rapid amplification cDNA ends primer extension show different N-terminal protein sequences arise through are regulated activity postnatal age. Heterogeneity in C-terminal arises splicing. Overexpression...
Abstract The set of proteins required for mitotic division remains poorly characterized. Here, an extensive series correlation analyses human and mouse transcriptomics data were performed to identify genes strongly reproducibly associated with cells undergoing S/G2-M phases the cell cycle. In so doing, 701 cycle-associated defined while it was shown that many are only expressed during these phases, expression others is also driven by alternative promoters. Of this list, 496 have known cycle...
The European honey bee (Apis mellifera) plays a major role in pollination and food production. Honey health is complex product of the environment, host genetics associated microbes (commensal, opportunistic pathogenic). Improved understanding these factors will help manage modern challenges to health. Here we used DNA sequencing characterise genomes metagenomes 19 colonies from across Britain. Low heterozygosity was observed many Scottish which had high similarity native dark bee. Colonies...
Abstract Acute liver failure is a rapidly progressing, life-threatening condition most commonly caused by an overdose of acetaminophen (paracetamol). The antidote, N-acetylcysteine (NAC), has limited efficacy when injury established. If acute damage severe, can develop with associated high mortality rates. We have previously demonstrated that alternatively, activated macrophages are potential therapeutic option to reverse in pre-clinical models. In this paper, we present data using...
Glial cell line-derived neurotrophic factor, GDNF, is vital to the development and maintenance of neural tissues; it promotes survival sympathetic, parasympathetic spinal motor neurons during development, protects midbrain dopaminergic from apoptosis well enough be a promising treatment for Parkinson's disease, controls renal testicular development. Understanding how GDNF interacts with its target cells therefore priority in several fields. Here we show that requires glycosaminoglycans as...
Abstract Cilia are complex microtubule-based organelles essential to a range of processes associated with embryogenesis and tissue homeostasis. Mutations in components these or those involved their assembly may result diverse set diseases collectively known as ciliopathies. Accordingly, many cilia-associated proteins have been described, while distinguishing cilia subtypes poorly defined. Here we out define genes motile humans based on transcriptional signature. To the signature, performed...
The action potential is an explosive depolarization of the plasma membrane. It can be driven by influx calcium ions through voltage-gated channels, as in sea urchin egg, or sodium human nerve cells. Action potentials travel rapidly along long cell processes called axons, especially if axon insulated myelin. amplitude a signal coded frequency potentials, all which have same amplitude.
There is a need for formalised diagrams that both summarise current biological pathway knowledge and support modelling approaches explain predict their behaviour. Here, we present new, freely available framework includes biologist-friendly language (mEPN), simple but sophisticated method to model parameterisation using information; stochastic flow algorithm simulates the dynamics of activity; 3-D visualisation engine aids understanding complexities system’s dynamics. We example models...
Abstract Macrophages respond to the TLR4 agonist LPS with a sequential transcriptional cascade controlled by complex regulatory network of signaling pathways and transcription factors. At least two distinct are currently known be engaged distinguished their dependence on adaptor molecule MyD88. We have used gene expression microarrays define effects each three variables—LPS dose, versus IFN-β -γ, genetic background—on response mouse BMDMs. Analysis correlation networks generated from data...
The development of ordered connections or “maps” within the nervous system is a common feature sensory systems and crucial for their normal function. NMDA receptors are known to play key role in formation these maps; however, intracellular signaling pathways that mediate effects glutamate poorly understood. Here, we demonstrate SynGAP, synaptic Ras GTPase activating protein, essential anatomical whisker-related patterns developing somatosensory rodent forebrain. Mice lacking SynGAP show only...
The natural distribution of the honeybee (Apis mellifera L.) has been changed by humans in recent decades to such an extent that formerly widest-spread European subspecies, Apis mellifera, is threatened extinction through introgression from highly divergent commercial strains large tracts its range. Conservation efforts for A. m. are underway multiple countries requiring reliable and cost-efficient molecular tools identify purebred colonies. Here, we developed four ancestry-informative SNP...
Tail amputation by tail docking or as an extreme consequence of biting in commercial pig production potentially has serious implications for animal welfare. causes peripheral nerve injury that might be associated with lasting chronic pain. The aim this study was to investigate the short- and long-term effects pigs on caudal DRG gene expression at different stages development, particularly relation genes nociception Microarrays were used analyse whole transcriptomes from amputated...
Patterning of the mouse somatosensory cortex is unusually evident because presence a “barrel field.” Presynaptic serotonin and postsynaptic glutamate receptors regulate barrel formation, but little known intracellular signaling pathways through which they act. To determine whether protein kinase A (PKA) plays role in development field, we examined five viable PKA subunit-specific knock-out (KO) lines for field abnormalities. Barrels are present these mice, those lacking RIIβ subunit display...
Abstract Several lines of evidence link macrophage activation and inflammation with (monoaminergic) nervous systems in the etiology depression. IFN treatment is associated depressive symptoms, whereas anti-TNFα therapies elicit positive mood. This study describes actions 2 monoaminergic antidepressants (escitalopram, nortriptyline) 3 anti-inflammatory drugs (indomethacin, prednisolone, antibody) on response human monocyte-derived macrophages (MDMs) from 6 individuals to LPS or IFN-α....
Vertebrate kidney organogenesis is characterised by the successive formation of pronephros, mesonephros and metanephros. The pronephros first to form functional embryonic lower vertebrates; although it vestigial in higher vertebrates, a necessary precursor for other types. Xenopus simple paired organ; each nephron consists single large glomus, one set tubules duct. organisation amenability laevis embryos manipulation make an attractive system which study organogenesis. It has been shown that...
Although the functions of porcine respiratory and reproductive syndrome virus (PRRSV) proteins are increasingly understood, roles host factors in modifying infection less well understood. Growing evidence places deubiquitination at core a multitude regulatory processes, ranging from cell growth to innate immune response health, such as cancer, degenerative infectious diseases. This report provides further information on functional role ubiquitin-specific peptidase 18 (USP18) during responses...
Many reptiles do not have heteromorphic sex chromosomes and for these species is determined during embryogenesis by the temperature of egg incubation rather than at conception. The phenomenon temperature-dependent determination (TSD) was discovered almost thirty years ago, but few advances been made towards elucidation its mechanism. In past substantial progress has in understanding molecular basis XY chromosomal (genetic) (GSD) through discovery SRY. It now possible to start comparing TSD...
Two natural neural inducing sources have been used, the notochord and somites together with growth factor bFGF, to investigate anterior/posterior patterning of tissue in an animal cap explant model Xenopus laevis. Notochord somite from stages 12.5/13 16, respectively, were manually isolated, combined heterochronically responding ectoderm aged gastrula stages. Somite recombinants also constructed caps injected noggin mRNA. The responses analyzed by reverse transcription polymerase chain...
Adoptive immunotherapy with Epstein-Barr virus (EBV)-specific T cells is an effective treatment for relapsed or refractory EBV-induced post-transplant lymphoproliferative disorders (PTLD) overall survival rates of up to 69%. EBV-specific have been conventionally made by repeated stimulation EBV-transformed lymphoblastoid cell lines (LCL), which act as antigen-presenting cells. However, this process expensive, takes many months, and has practical risks associated live virus. We developed a...
Abstract The set of proteins required for mitotic division remains poorly characterised. Here, an extensive series correlation analyses human and mouse transcriptomics data was performed to identify genes strongly reproducibly associated with cells undergoing S/G2-M phases the cell cycle. In so doing, a list 701 cycle-associated defined shown that whilst many are only expressed during these phases, expression others is also driven by alternative promoters. Of this list, 496 have known cycle...