A5016876250- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- Protein Structure and Dynamics
- DNA Repair Mechanisms
- Maritime Ports and Logistics
- Microtubule and mitosis dynamics
- Maritime Transport Emissions and Efficiency
- Transgenic Plants and Applications
- Bacterial Genetics and Biotechnology
- Maritime Navigation and Safety
- Microbial Metabolic Engineering and Bioproduction
- Bioinformatics and Genomic Networks
- Bacteriophages and microbial interactions
- Climate Change Policy and Economics
- RNA Interference and Gene Delivery
- Enzyme Structure and Function
- Gene Regulatory Network Analysis
- Viral Infectious Diseases and Gene Expression in Insects
- Glycosylation and Glycoproteins Research
- Fungal and yeast genetics research
- Mass Spectrometry Techniques and Applications
Conservatoire National des Arts et Métiers
2019-2023
Laboratoire de Biochimie Théorique
2014-2021
Université Paris Cité
2015-2019
Centre National de la Recherche Scientifique
2015-2019
Sorbonne Université
2019
Génomique Bioinformatique et Applications
2019
Roslin Institute
2016
University of Edinburgh
2016
Sorbonne Paris Cité
2015
Délégation Paris 7
2015
RecA protein is the prototypical recombinase. Members of recombinase family can accurately repair double strand breaks in DNA. They also provide crucial links between pairs sister chromatids eukaryotic meiosis. A very broad outline how these proteins align homologous sequences and promote DNA exchange has long been known, as are crystal structures RecA-DNA pre- postsynaptic complexes; however, little known about homology searching conformations details bacterial genomes rapidly searched...
There is a need for formalised diagrams that both summarise current biological pathway knowledge and support modelling approaches explain predict their behaviour. Here, we present new, freely available framework includes biologist-friendly language (mEPN), simple but sophisticated method to model parameterisation using information; stochastic flow algorithm simulates the dynamics of activity; 3-D visualisation engine aids understanding complexities system’s dynamics. We example models...
Oligomeric macromolecules in the cell self-organize into a wide variety of geometrical motifs such as helices, rings or linear filaments. The recombinase proteins involved homologous recombination present many assembly motifs. Here, we examine particular polymorphic characteristics RecA, most studied member family, using an integrative approach that relates local modes monomer/monomer association to global architecture their screw-type organization. In our approach, are sampled via docking...
Abstract Motivation Protein–protein docking aims at predicting the geometry of protein interactions to gain insights into mechanisms underlying these processes and develop new strategies for drug discovery. Interactive user-oriented manipulation tools can support this task complementary automated software. Results This article presents an interactive multi-body protein–protein software, UDock2, designed research but also usable teaching popularization science purposes due its high usability....
Homologous recombination is a fundamental process in all living organisms that allows the faithful repair of DNA double strand breaks, through exchange strands between homologous regions genome. Results three decades investigation and recent fruitful observations have unveiled key elements reaction mechanism, which proceeds along nucleofilaments recombinase proteins RecA family. Yet, one essential aspect has largely been overlooked when deciphering mechanism: while ATP hydrolyzed large...
The recognition and strand exchange process central to homologous recombination (HR) involves structural transitions for both the DNA protein filament components. engaged in HR ...
In vivo, RecA mediated homology recognition must stringently reject non-homologous pairings, but it also allow strand exchange to progress through regions within homologous pair...
Protein filaments are dynamic entities that respond to external stimuli by slightly or substantially modifying the internal binding geometries between successive protomers. This results in overall changes filament architecture, which difficult model due helical character of system. Here, we describe how distortions RecA nucleofilaments and their consequences on filament-DNA bound DNA-DNA interactions at different stages homologous recombination process can be modeled using PTools/Heligeom...