A5040789245- Receptor Mechanisms and Signaling
- Neurotransmitter Receptor Influence on Behavior
- Neuroscience and Neuropharmacology Research
- Olfactory and Sensory Function Studies
- RNA Research and Splicing
- Neuropeptides and Animal Physiology
- Parkinson's Disease Mechanisms and Treatments
- Neural dynamics and brain function
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Neuroscience and Neural Engineering
- Nuclear Receptors and Signaling
- Computational Drug Discovery Methods
- Diet and metabolism studies
- RNA regulation and disease
- Neurobiology and Insect Physiology Research
- Nerve injury and regeneration
- Genetic Neurodegenerative Diseases
- Biochemical Analysis and Sensing Techniques
- Adipose Tissue and Metabolism
- Neurological disorders and treatments
- Tryptophan and brain disorders
- Chemical Synthesis and Analysis
- Photoreceptor and optogenetics research
- Pharmacological Effects and Assays
Università degli Studi del Piemonte Orientale “Amedeo Avogadro”
2022-2024
Italian Institute of Technology
2015-2024
University of Genoa
2015
Leitat Technological Center
1996
Dopamine (DA) controls many vital physiological functions and is critically involved in several neuropsychiatric disorders such as schizophrenia attention deficit hyperactivity disorder. The major function of the plasma membrane dopamine transporter (DAT) rapid uptake released DA into presynaptic nerve terminals leading to control both extracellular levels intracellular stores DA. Here, we present a newly developed strain rats which gene encoding DAT knockout Rats (DAT-KO) has been disrupted...
The ability of dopamine receptors to interact with other receptor subtypes may provide mechanisms for modulating dopamine-related functions and behaviors. In particular, there is evidence suggesting that the trace amine-associated 1 (TAAR1) affects dopaminergic system by regulating firing rate neurons or altering D2 (D2R) responsiveness ligands. TAAR1 a Gα<sub>s</sub> protein-coupled activated biogenic amines, "trace amines," such as β-phenylethylamine (β-PEA) tyramine are normally found at...
Bioluminescence resonance energy transfer (BRET)-based biosensors have been extensively used over the last decade to study protein-protein interactions and intracellular signal transduction in living cells. In this review, we discuss various BRET that developed investigate biology, pharmacology, signaling of G protein-coupled receptors (GPCRs). GPCRs form two distinct types multiprotein complexes based upon their inclusion proteins or β-arrestins can be differentially affected by drugs...
Abstract: Several putative neurotransmitters and metabolites were monitored simultaneously in the extracellular space of neostriatum, substantia nigra, cortex subcutaneous tissue rat by vivo microdialysis. Glutamate (Glu) aspartate (Asp) at submicromolar γ‐aminobutyric acid (GABA) was nanomolar concentrations all brain regions. The highest concentration dopamine (DA) neostriatum. Dynorphin B (Dyn B) picomolar range Although no GABA, DA, or Dyn could be detected tissue, Glu Asp levels ≈5 ≈0.4...
Dopamine (3-hydroxytyramine) is a well-known catecholamine neurotransmitter involved in multiple physiological functions including movement control. Here we report that the major extracellular metabolite of dopamine, 3-methoxytyramine (3-MT), can induce behavioral effects dopamine-independent manner and these are partially mediated by trace amine associated receptor 1 (TAAR1). Unbiased vivo screening putative ligands for potential on control revealed 3-MT infused brain able to complex set...
Glial cell-derived neurotrophic factor (GDNF) has a potent action in promoting the survival of dopamine (DA) neurons. Several studies indicate that increasing GDNF levels may be beneficial for treatment Parkinson's disease (PD) by reducing neurodegeneration DA Despite plethora preclinical showing efficacy PD animal models, its application humans remains questionable poor and side effects due to uncontrolled, ectopic expression. Here we took advantage SINEUPs, new class antisense long...
Trace amine-associated receptors (TAARs) are a class of G protein-coupled found in mammals. While TAAR1 is expressed several brain regions, all the other TAARs have been described mainly olfactory epithelium and glomerular layer bulb believed to serve as new sensing innate odors. However, there evidence that TAAR5 could play role also central nervous system. In this study, we characterized mouse line lacking (TAAR5 knockout, TAAR5-KO) expressing beta-galactosidase mapping expression. We not...
Central dopamine plays a key role in sexual behavior. Recently, Dopamine-Transporter knockout (DAT KO) rat has been developed, which displays several behavioral dysfunctions that have related to increased extracellular levels and altered turnover secondary DAT gene silencing. This prompted us characterize the behavior of these KO rats their heterozygote (HET) wild type (WT) counterparts classical copulatory tests with sexually receptive female rat, verify if how acquisition experience...
Trace amine-associated receptor 1 (TAAR1) is a G protein-coupled that belongs to the family of TAAR receptors and responds class compounds called trace amines, such as β-phenylethylamine (β-PEA) 3-iodothyronamine (T(1)AM). The known have very rich pharmacology could be also activated by other classes compounds, including adrenergic serotonergic ligands. It expected targeting TAAR1 provide novel pharmacological approach correct monoaminergic dysfunctions found in several brain disorders,...
Trace amine associated receptor 1 (TAAR1) is a G protein coupled (GPCR) expressed in brain and periphery activated by wide spectrum of agonists that include, but are not limited to, trace amines (TAs), amphetamine-like psychostimulants, endogenous thyronamines such as thyronamine (T0AM) 3-iodothyronamine (T1AM). Such polypharmacology has made it challenging to understand the role biology TAAR1. In an effort molecular basis TAAR1 activation, we rationally designed synthesized small family...
G protein-coupled trace amine-associated receptor 1 (TAAR1) is expressed in several brain regions and modulates dopaminergic activity partially by affecting D2 dopamine function. In vitro , the nonselective agonist apomorphine can activate mouse rat TAAR1. The aim of present study was to evaluate whether at rodent TAAR1 observed studies contributes its behavioral manifestation mice. For this purpose, we compared effects a wide range doses wild type (WT) knockout (TAAR1-KO)...
Spinobulbar muscular atrophy (SBMA) is an X-linked neuromuscular disease caused by polyglutamine (polyQ) expansion in the androgen receptor (AR) gene. SBMA belongs to family of polyQ diseases, which are fatal neurodegenerative disorders mainly protein-mediated toxic gain-of-function mechanisms and characterized deposition misfolded proteins form aggregates. The neurotoxicity can be modified phosphorylation at specific sites, thereby providing rationale for development disease-specific...
Abstract Friedreich's ataxia (FRDA) is an untreatable disorder with neuro- and cardio-degenerative progression. This monogenic disease caused by the hyper-expansion of naturally occurring GAA repeats in first intron FXN gene, encoding for frataxin, a protein implicated biogenesis iron-sulfur clusters. As genetic defect interferes transcription, FRDA patients express normal frataxin but at insufficient levels. Thus, current therapeutic strategies are mostly aimed to restore physiological...
Trace Amine-Associated Receptor 1 (TAAR1) is a G protein-coupled receptor that expressed in brain and periphery responds to class of compounds called trace amines, such as β-phenylethylamine (β-PEA), tyramine, tryptamine, octopamine. The known have very rich pharmacology could be also activated by different classes compounds, including dopaminergic, adrenergic serotonergic ligands. It expected targeting hTAAR1 provide novel pharmacological approach for several human disorders, schizophrenia,...
Neuronal plasticity produces changes in excitability, synaptic transmission, and network architecture response to external stimuli. Network adaptation environmental conditions takes place time scales ranging from few seconds days, modulates the entire dynamics. To study defined long-term experimental protocols, we setup a system that combines optical electrophysiological tools embedded cell incubator. Primary hippocampal neurons transduced with lentiviruses expressing...
The computational study here proposed allowed us to discovery for the first time two TAAR5 antagonist, selective over TAAR1 receptor.