A5082505630- Lanthanide and Transition Metal Complexes
- Radioactive element chemistry and processing
- Blood properties and coagulation
- Magnetism in coordination complexes
- Metal complexes synthesis and properties
- Celiac Disease Research and Management
- Crystallization and Solubility Studies
- Advanced MRI Techniques and Applications
- X-ray Diffraction in Crystallography
- Chemical Synthesis and Characterization
- Molecular Sensors and Ion Detection
- Erythrocyte Function and Pathophysiology
- Microscopic Colitis
- Phagocytosis and Immune Regulation
- Tendon Structure and Treatment
- Platelet Disorders and Treatments
- Organoboron and organosilicon chemistry
- Chemical Synthesis and Analysis
- Chemical Thermodynamics and Molecular Structure
- Plant Surface Properties and Treatments
- Cell death mechanisms and regulation
- Boron Compounds in Chemistry
- Inorganic and Organometallic Chemistry
- Transgenic Plants and Applications
- Autoimmune Bullous Skin Diseases
University of Debrecen
2015-2025
University of Prešov
2020-2022
Institute of Political Science
2020-2022
Hungarian Academy of Sciences
2006-2011
The University of Texas Southwestern Medical Center
2004-2008
Advanced Imaging Research (United States)
2008
Rogers (United States)
2004-2007
The University of Texas at Dallas
1992
University of Virginia
1982
<b>Background:</b> Some patients with untreated coeliac disease are negative for serum endomysial autoantibodies (EmA) targeted against transglutaminase 2 (TG2). <b>Aims:</b> To evaluate the clinical and histological features of EmA-negative disease, to examine whether EmA-equivalent TG2 can be seen in small-bowel mucosa when absent serum. <b>Patients:</b> Serum EmA was studied 177 biopsy-proved specimens from adult disease. 20 intestinal diseases served as non-coeliac controls; three had...
The pH-sensitive contrast agent, GdDOTA-4AmP (Gd1) has been successfully used to map tissue pH by MRI. Further studies now demonstrate that two distinct chemical forms of the complex can be prepared depending upon at which Gd(3+) is mixed with ligand 1. desired form this complex, referred here as a Type II obtained exclusive product only when complexation reaction performed above 8. At lower values, second formed that, analogy an intermediate during preparation GdDOTA, we tentatively assign...
Transglutaminase 2 (TG2) is an inducible transamidating acyltransferase that catalyzes Ca(2+)-dependent protein modifications. In addition to being enzyme, TG2 also serves as a G for several seven transmembrane receptors and acts co-receptor integrin β1 β3 integrins distinguishing it from other members of the transglutaminase family. ubiquitously expressed in almost all cell types compartments, present on surface gets secreted extracellular matrix via non-classical mechanisms. has been...
The multifunctional tissue transglutaminase 2 (TG2) has a four-domain structure with several Ca(2+)-regulated biochemical activities, including transglutamylation and GTP hydrolysis. of the Ca(2+)-binding form human enzyme is not known, its sites have been fully characterized. By mutagenesis, we targeted active site Cys, three based on homology to residues epidermal factor XIIIa (S1-S3), two regions negative surface potentials (S4 S5). CD spectroscopy, antibody-binding assay GTPase activity...
The multifunctional, protein cross-linking transglutaminase 2 (TG2) is the main autoantigen in celiac disease, an autoimmune disorder with defined etiology. Glutamine-rich gliadin peptides from ingested cereals, after their deamidation by TG2, induce T-lymphocyte activation accompanied autoantibody production against TG2 1–2% of population. pathogenic role and exact binding properties these antibodies to are still unclear. Here we show that different patients target same conformational...
The endomysial antibody (EMA) immunofluorescent test is a highly specific method to detect disease-specific autoantibodies in celiac disease (CD) by their binding natural transglutaminase-2 autoantigen tissue sections, and it used as compulsory confirmatory the non-invasive diagnosis of CD. classical EMA substrates are monkey esophagus human umbilical cord. It increasingly difficult use these tissues due ethical concerns animal welfare regulations. In this study, we developed, cell culture,...
A cyclen-based ligand containing trans-acetate and trans-methylenephosphonate pendant groups, H6DO2A2P, was synthesized its protonation constants (12.6, 11.43, 5.95, 6.15, 2.88, 2.77) were determined by pH-potentiometry 1H NMR spectroscopy. The first two protonations shown to occur at the macrocyclic ring N–CH2–PO32– nitrogens while third fourth phosphonate groups. In parallel with of −PO32– protons from NH+−CH2−PO32– are transferred N−CH2−COO– nitrogens. stability Ca2+, Cu2+, Zn2+ (ML, MHL,...
Necroptosis is a regulated necrotic-like cell death modality which has come into the focus of attention since it known to contribute pathogenesis many inflammatory and degenerative diseases as well tumor regulation. Based on current data, necroptosis serves backup mechanism when receptor-induced apoptosis inhibited or absent. However, necroptotic role proteins involved in mitochondrial not been investigated. Here, we demonstrated that stimulation several pattern recognition receptors induced...
The protonation constants (K iH) of 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (H4dota) were redetermined at 25 °C in 0.1 M NMe4Cl. log Ki H values (i = 1–5) are 12.6, 9.70, 4.50, 4.14 and 2.32, respectively. stability [Ce(dota)]– [Yb(dota)]– determined by pH-potentiometry as KCeL 24.6 KYbL 26.4. formation rates studied the stopped-flow method much higher pH than before. In range 3.5–9.3 spectra interpreted terms diprotonated, [Ce(H2dota)]+, (at around > 8) monoprotonated,...
Addition of a benzyl substituent to the macrocyclic ring DOTA has substantial impact on conformational flipping motion macrocycle in resulting LnDOTA complexes. The p-NO2-benzyl Ln(p-NO2-Bn-DOTA)- complexes lies an equatorial position and effectively "locks" conformation into δδδδ configuration. presence group also increases population square antiprismatic (SAP) coordination isomer for all relative that seen respective LnDOTA- Despite this increase SAP population, rate water exchange these...
Summary Background : Immunoglobulin A class transglutaminase autoantibodies are highly predictive markers of active coeliac disease, a disorder difficult to recognize solely on clinical grounds. Aims To develop and evaluate simple rapid test for point‐of‐care detection autoantibodies. Methods The novel whole blood utilizes the patient's endogenous in red cells transglutaminase‐specific immunoglobulin antibodies present sample, with normal plasma as positive control. We evaluated 284 patients...
TG2 (transglutaminase 2) is a calcium-dependent protein cross-linking enzyme which involved in variety of cellular processes. The threshold level calcium needed for endogenous and recombinant activity has been controversial, the former being more sensitive to than latter. In present study we address this question by identifying single amino acid change from conserved valine glycine at position 224 compared with sequence available genomic databases. Substituting residue Gly224 increased its...
Macrophage polarization is a process whereby macrophages develop specific phenotype and functional response to different pathophysiological stimuli tissue environments. In general, two main macrophage phenotypes have been identified: inflammatory (M1) alternatively activated (M2) characterized specifically by IL-1β IL-10 production, respectively. the cardiotoxin-induced skeletal muscle injury model bone marrow-derived (BMDMs) play central role in regulating repair. Bone monocytes arriving at...
Several Bi(III) complexes are used in medicine as drugs. Bi(DO3A-Bu) has recently been proposed a nonionic contrast agent X-ray imaging (H3DO3A-Bu = 10-[2,3-dihydroxy-(1-hydroxymethyl)propyl]-1,4,7,10-tetraazacyclododecane-1,4,7,-triacetic acid). The solution equilibria and NMR structure dynamics of the similar Bi(DOTA)- have investigated (H4DOTA 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic stability constants were determined with study competition between Br- ions ligands DOTA or...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and complexation properties of a new macrocyclic polyaza polyphosphinate ligand, DOTEP (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetrakis(methyleneethylphosphinate))Istvan Lazar, A. Dean Sherry, Ravichandran Ramasamy, Erno Brucher, Robert KiralyCite this: Inorg. Chem. 1991, 30, 26, 5016–5019Publication Date (Print):December 1, 1991Publication History Published online1 May 2002Published inissue 1 December...
ABSTRACT Objective: Deamidated gliadin peptides are efficient antigens in diagnostic tests for celiac disease, and results correlate better with transglutaminase 2–based assays than those native gliadin. We investigated whether deamidated structurally similar to 2 or could mimic epitopes. Patients Methods: Serum samples from 74 65 control patients, 13 different 2–specific monoclonal mouse antibodies were their binding commercially available using enzyme‐linked immunosorbent assay,...
Abstract Formation of ternary complexes between Gd‐DTPA, Gd‐DTPA‐BMA, and Gd‐DOTA, used as contrast enhancement agents in MRI the endogenously available carbonate phosphate ions, has been demonstrated. The extent complex formation its effect on proton relaxation, measured at 9 MHz, rates is negligible around pH <8. Gd‐EDTA forms more stable with it also strongly coordinates terdentate citrate ligand. Gd‐EDTA( X ) ( = CO 3 2‐ , Cit 3‐ results a significant decrease relaxation under...