A5103014310- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Neurogenesis and neuroplasticity mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Olfactory and Sensory Function Studies
- Memory and Neural Mechanisms
- Ion channel regulation and function
- Neuroendocrine regulation and behavior
- Sleep and Wakefulness Research
- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- Stress Responses and Cortisol
- Neurobiology and Insect Physiology Research
- Herpesvirus Infections and Treatments
- Biochemical Analysis and Sensing Techniques
- Regulation of Appetite and Obesity
- Photoreceptor and optogenetics research
- Pain Mechanisms and Treatments
- Genetics and Neurodevelopmental Disorders
- Zebrafish Biomedical Research Applications
- Adipose Tissue and Metabolism
- Cannabis and Cannabinoid Research
- Neuroscience of respiration and sleep
- Toxoplasma gondii Research Studies
- Nicotinic Acetylcholine Receptors Study
HUN-REN Institute of Experimental Medicine
2013-2023
Hungarian Academy of Sciences
2009-2023
Czech Academy of Sciences, Institute of Experimental Medicine
2021
Semmelweis University
2018-2019
University of Szeged
2019
Hungarian Research Network
2009
Research Network (United States)
2009
University of Debrecen
2005
University of Glasgow
2005
University of Baltimore
2003
Abstract Olfactory sensory information is processed and integrated by circuits within the olfactory bulb. Golgi morphology suggests bulb contains several major neuronal classes. However, an increasingly diverse collection of neurochemical markers have been localized in subpopulations neurons. While mouse becoming animal model choice for research, little known about proportions neurons expressing coexpressing different this species. Here we characterize populations main bulb, focusing on...
Gamma frequency oscillations in cortical regions can be recorded during cognitive processes, including attention or memory tasks. These are generated locally as a result of reciprocal interactions between excitatory pyramidal cells and perisomatic inhibitory interneurons. Here, we examined the contribution three interneuron types—the parvalbumin-containing fast-spiking basket (FSBCs) axo-axonic (AACs), well cholecystokinin-containing regular-spiking (RSBCs) to cholinergically induced...
Neurotransmitter release at most central synapses is synchronized to the timing of presynaptic action potentials. Here, we show that three classes depolarization-induced suppression inhibition-expressing, cholecystokinin (CCK)-containing, hippocampal interneurons highly asynchronous in response trains This asynchrony correlated class interneuron but unrelated their postsynaptic cell target. Asynchronous and synchronous from CCK-containing a slightly different calcium dependence, such...
Hippocampal sharp waves and the associated ripple oscillations (SWRs) are implicated in memory processes. These network events emerge intrinsically CA3 network. To understand cellular interactions that generate SWRs, we detected first spiking activity followed by recording of synaptic currents distinct types anatomically identified neurons during SWRs occurred spontaneously mouse hippocampal slices. We observed vast majority interneurons fired whereas only a small portion pyramidal cells was...
Cholinergic neuromodulation of hippocampal circuitry promotes network oscillations and facilitates learning memory through cellular actions on both excitatory inhibitory circuits. Despite widespread recognition that neurochemical content discriminates between functionally distinct interneuron populations, there has been no systematic examination whether neurochemically classes undergo differential cholinergic in the hippocampus. Using GFP transgenic mice enable visualization perisomatically...
Anatomical studies have shown that the G-protein-coupled cannabinoid receptor-1 (CB 1 ) is selectively expressed in a subset of GABAergic interneurons. It has been proposed these cells regulate rhythmic activity and play key role mediating cognitive actions marijuana endogenous cannabinoids. However, physiology, anatomy, synaptic connectivity neocortical CB -expressing interneurons remain poorly studied. We identified population layer II/III mouse slices. These were multipolar or bitufted,...
Presynaptic inhibition of primary muscle spindle (group Ia) afferent terminals in motor nuclei the spinal cord plays an important role regulating output and is produced by a population GABAergic axon known as P boutons. Despite extensive investigation, cells that mediate this control have not yet been identified. In work, we use immunocytochemistry with confocal microscopy EM to demonstrate boutons can be distinguished from other ventral horn rat mouse their high level glutamic acid...
Although endocannabinoids have emerged as essential retrograde messengers in several forms of synaptic plasticity, it remains controversial whether they mediate long-term depression (LTD) glutamatergic synapses onto excitatory and inhibitory neurons the hippocampus. Here, we show that parvalbumin- somatostatin/metabotropic glutamate receptor 1 a (mGlu 1a )-positive GABAergic interneurons express diacylglycerol lipase-α (DGL-α), synthesizing enzyme endocannabinoid 2-arachidonoylglycerol...
High titers of autoantibodies to glutamic acid decarboxylase (GAD) are well documented in association with stiff person syndrome (SPS). Glutamic is the rate-limiting enzyme synthesis γ-aminobutyric (GABA), and impaired function GABAergic neurons has been implicated pathogenesis SPS. Autoantibodies GAD might be causative agent or a disease marker.To investigate characteristics potential pathogenicity patients SPS related disorders.Retrospective cohort study laboratory investigation.Weatherall...
Key points Lateral hypothalamus (LH) contains GABA neurons involved in controlling metabolism and sleep. LH glutamic acid decarboxylase 65 (GAD65) are intrinsically depolarized, unlike classical GAD65 of the cortex. distinct from most studied (orexin melanin‐concentrating hormone cells). A subset glucose inhibited. Our study adds new populations sensing to list hypothalamic sugar sensors introduces inhibitory circuit elements LH. Abstract GABAergic vital for brain function. Their...
Abstract Breast cancer and melanoma are among the most frequent types leading to brain metastases. Despite unquestionable clinical significance, important aspects of development secondary tumours central nervous system largely uncharacterized, including extravasation metastatic cells through blood‐brain barrier. By using transmission electron microscopy, here we followed interactions endothelial during adhesion, intercalation/incorporation transendothelial migration steps. We observed that...
Distinct networks of γ-aminobutyric acidergic interneurons connected by electrical synapses can promote different patterns activity in the neocortex. Cannabinoids affect memory and cognition powerfully modulating a subset inhibitory synapses; however, very little is known about synaptic properties cannabinoid receptor–expressing neurons (CB1-positive irregular spiking [CB1-IS]) Using paired recordings neocortical slices, we 1st report here that CB1-IS cells, but not fast-spiking (FS) are...
In the mammalian hippocampus, neurotransmitter acetylcholine (ACh) promotes learning and memory storage. During sensory processing learning, large ACh-dependent electrical oscillatory events are observed, which involve synchronization of both inhibitory excitatory neural circuits. While actions ACh known on hippocampal circuits, its specific circuits poorly understood. We show that two types cholecystokinin-positive local circuit interneuron, so-called 'basket cells' 'Schaffer...
Release of acetylcholine (ACh) in the hippocampus (HC) occurs during exploration, arousal, and learning. Although medial septum-diagonal band Broca (MS-DBB) is major extrinsic source cholinergic input to HC, neurons intrinsic HC also exist but remain poorly understood. Here, ChAT-tauGFP ChAT-CRE/Rosa26YFP (ChAT-Rosa) mice were examined HC. The was densely innervated with GFP-positive axons, often accompanied by large structures, some which Neurotrace/DAPI-negative likely represent axon...
In cortical structures, principal cell activity is tightly regulated by different GABAergic interneurons (INs). Among these INs are vasoactive intestinal polypeptide-expressing (VIP+) INs, which innervate preferentially other providing a structural basis for temporal disinhibition of cells. However, relatively little known about VIP+ in the amygdaloid basolateral complex (BLA). this study, we report that have variable density distinct subdivisions mouse BLA. Based on anatomical,...
Stress-induced cortical alertness is maintained by a heightened excitability of noradrenergic neurons innervating, notably, the prefrontal cortex. However, neither signaling axis linking hypothalamic activation to delayed and lasting nor molecular cascade gating noradrenaline synthesis defined. Here, we show that corticotropin-releasing hormone-releasing innervate ependymal cells 3rd ventricle induce ciliary neurotrophic factor (CNTF) release for transport through brain's aqueductal system....
Necroptosis is a regulated necrotic-like cell death modality which has come into the focus of attention since it known to contribute pathogenesis many inflammatory and degenerative diseases as well tumor regulation. Based on current data, necroptosis serves backup mechanism when receptor-induced apoptosis inhibited or absent. However, necroptotic role proteins involved in mitochondrial not been investigated. Here, we demonstrated that stimulation several pattern recognition receptors induced...
In developing cerebellar interneurons, NMDA increases spontaneous GABA release by activating presynaptic receptors. We investigated the role of these receptors on differentiating basket/stellate cells in cultures grown under conditions allowing functional synaptic transmission. Presynaptic GABAergic boutons were visualized either GAD65 immunostaining or using derived from GAD65-enhanced green fluorescent protein (eGFP) transgenic mice, which express eGFP. After first week culture, whole-cell...
Abstract Commissural interneurons (CINs) are a necessary component of central pattern generators (CPGs) for locomotion because they mediate the coordination left and right muscle activity. The projection patterns relative locations different classes CINs in ventromedial part rodent lumbar cord have been described (Eide et al. [ 1999 ] J Comp Neurol 403:332–345; Stokke 2002 446:349–359; Nissen 2005 483:30–47). However, distribution prevalence CIN neurotransmitter phenotypes ventral region...
Most cortical neurons fire regularly when excited by a constant stimulus. In contrast, irregular-spiking (IS) interneurons are remarkable for the intrinsic variability of their spike timing, which can synchronize amongst IS cells via specific gap junctions. Here, we have studied biophysical mechanisms this irregular spiking in mice, and how context synchronous network oscillations. Using patch-clamp recordings, artificial dynamic conductance injection, pharmacological analysis computational...