Abstract Limited self-regenerating capacity of human skeleton makes the reconstruction critical size bone defect a significant challenge for clinical practice. Aimed regenerating tissues, this study was designed to investigate osteogenic differentiation, along with repair 3D chitosan (CHT) scaffolds enriched graphene oxide (GO) in critical-sized mouse calvarial defect. Histopathological/histomorphometry and scanning electron microscopy(SEM) analysis implants revealed larger amount new...

Diabetic retinopathy, a secondary complication of diabetes mellitus, can lead to irreversible vision loss. Currently, no treatment is approved for early phases diabetic retinopathy. Modifications the expression pattern miRNAs could be involved in retinal damage subjects. Therefore, we aimed at identification dysregulated miRNAs-mRNA interactions that might biomarkers and pharmacological targets diagnosis

This study aimed to develop and compare single multiple 3D models such as multicellular tumor spheroids investigate the influence of Matrigel on their morphological functional behavior. MDA-MB-231 were generated in presence absence key biological properties within 6 days culture monitored. Our results revealed formation well-defined Matrigel, with a uniform morphology, increased diameter, good circularity, expression proliferation marker (PCNA). In comparison, without characterized by an...

The role of the hydroxycarboxylic acid receptor 2 (HCA2) in retinal damage induced by diabetes has never been explored. In this context, present study highlights an upregulation HCA2 receptors diabetic C57BL6J mice. Moreover, we illustrate that exert anti-inflammatory effect on when activated endogenous ligand β-hydroxybutyrate.Seven-to-10-week-old mice were rendered a single intraperitoneal injection streptozotocin (75 mg/kg body weight) and monitored intermittently over 10-week period...

Bone regeneration is a claim challenge in addressing bone defects with large tissue deficits, that involves grafts to support the activity. In vitro biocompatibility of bacterial cellulose-modified polyhydroxyalkanoates (PHB/BC) scaffolds and its osteogenic potential critical-size mouse calvaria had been investigated. promotion mineralization were analyzed by biochemistry, histology/histomorphometry, X-ray analysis immunofluorescence for highlighting osteogenesis markers. summary, our...

The blood-brain barrier (BBB) is the main interface controlling molecular and cellular traffic between central nervous system (CNS) periphery. It consists of cerebral endothelial cells (CECs) interconnected by continuous tight junctions, closely associated pericytes astrocytes. Different parts CNS have diverse functions structures may be subject different pathologies, in which BBB actively involved. largely unknown, however, what are differences regions brain. Using silico, vitro, ex vivo...

Polyethylene terephthalate (PET) is a major pollutant polymer, due to its wide use in food packaging and fiber production industries worldwide. Currently, there great interest for recycling the huge amount of PET-based materials, derived especially from textile industries. In this study, we applied electrospinning technique obtain nanostructured fibrillary membranes based on PET materials. Subsequently, recycled networks were decorated with silver nanoparticles through chemical reduction...

Abstract Breast cancer and melanoma are among the most frequent types leading to brain metastases. Despite unquestionable clinical significance, important aspects of development secondary tumours central nervous system largely uncharacterized, including extravasation metastatic cells through blood‐brain barrier. By using transmission electron microscopy, here we followed interactions endothelial during adhesion, intercalation/incorporation transendothelial migration steps. We observed that...

The side effects associated with the chemotherapy of triple-negative breast cancer (TNBC), such as nucleotide-binding oligomerization domain (NOD)-like receptor family (NLR), pyrin containing 3 (NLRP3) inflammasome activity, are responsible for treatment failure and high mortality rates. Therefore, advanced delivery systems have been developed to improve transport targeted administration anti-tumor agents at tumor sites using tissue engineering approaches. Implantable based on biodegradable...

Plasmalemma vesicle-associated protein (PLVAP) regulates transcytosis in vascular endothelial cells. PLVAP expression is increased pathological conditions, such as diabetic retinopathy. P2X7 receptor antagonists have been shown to preserve blood-retinal barrier (BRB) integrity. Here, we tested the hypothesis that tightly linked activity, leading breakdown of BRB an vitro model We integrated network approaches with retinopathy using primary human retinal microvascular cells (HRMECs). Cells...

Bisphenol A (BPA) is an endocrine-disrupting chemical released into the environment, with severe consequences for human health, including metabolic syndrome and associated pathological conditions. Due to limited information on BPA-induced hepatotoxicity, present study focused investigating association between toxicity inflammatory markers in liver, how these injuries may be alleviated using natural agent silymarin, a flavonoid antioxidant properties obtained from Silybum marianum....

Inflammatory bowel disease (IBD) represents a group of chronic autoimmune and idiopathic disorders that are characteristic industrialized countries. In contrast to drug therapies, which exert several side effects, herbal remedies have constantly attracted the attention researchers. Therefore, in present study, mother tincture (MT) from fresh, young, non-woody Thuja occidentalis L. branches with leaves was obtained using distillation-based techniques. Further, this used assess its vitro vivo...

Since cadmium is a toxic metal that can cause serious health problems for humans, it necessary to find bioremediation solutions reduce its harmful effects. The main goal of our work was develop functional food based on elemental selenium nanoparticles (SeNPs) obtained by green synthesis using Lactobacillus casei and validate their ability annihilate the hepatic effects induced cadmium. characterization SeNPs assessed UV-Vis spectroscopy, FTIR, XRD, DLS TEM. In order investigate...

This study assessed the distribution of heavy metals in gills, kidney, and liver, correlated with severity histopathological changes, three fish species different feeding habitats (Barbus barbus, Squalius cephalus, Chondrostoma nasus) from Crișul Negru river, Romania. The levels copper (Cu), chromium (Cr), cadmium (Cd), lead (Pb), zinc (Zn) tissues were measured by atomic absorption spectrophotometry. Histopathology expressions TNF-α proliferation cell nuclear antigen (PCNA) investigated...

Liver fibrosis can develop on the background of hyperglycemia in diabetes mellitus. However, xenobiotic-related factors may accelerate diabetes-associated liver fibrosis. In this study, we aimed to assess antfibrotic effect ADSC and HGF therapy establish cellular molecular mechanisms through vitro vivo experiments. vitro, TGF-β1-activated hepatic stellate cells (HSCs) were cocultured with ADSCs or HGF, expression several markers was investigated. The antifibrotic ADSCs, supplemented further...

Hydrogel-based dressings exhibit suitable features for successful wound healing, including flexibility, high water-vapor permeability and moisture retention, exudate absorption capacity. Moreover, enriching the hydrogel matrix with additional therapeutic components has potential to generate synergistic results. Thus, present study centered on diabetic healing using a Matrigel-enriched alginate embedded polylactic acid (PLA) microspheres containing hydrogen peroxide (H2O2). The synthesis...

Nanomaterials (NMs) can be produced in plenty of variants posing several challenges for NM hazard and risk assessment. Metabolomic profiling NM-treated cells tissues allows insights into underlying Mode-of-Action (MoA) offers advantages this context. It supports the description Adverse Outcome Pathways (AOPs) and, therefore, tailored AOP-based testing strategies. Moreover, it bears great potential biomarker discovery supporting toxicity prediction. Here, we applied metabolomics to treated...

Over the years, natural-based scaffolds have presented impressive results for bone tissue engineering (BTE) application. Further, outstanding interactions been observed during interaction of graphene oxide (GO)-reinforced biomaterials with both specific cell cultures and injured in vivo experimental conditions. This research hereby addresses potential fish gelatin/chitosan (GCs) hybrids reinforced GO to support vitro osteogenic differentiation and, further, investigate its behavior when...

Background: Diabetic retinopathy (DR) is a neurovascular disease, characterized by deficiency of brain-derived neurotrophic factor (BDNF), regulator autophagy. Beta-hydroxybutyrate (BHB), previously reported as protective agent in DR, has been associated with BDNF promotion. Here, we investigated whether systemic BHB affects the retinal levels and local autophagy diabetic mice retinopathy; Methods: C57BL/6J were administered intraperitoneal (i.p.) streptozotocin (STZ) (75 mg/kg) injection to...

During parenchymal brain metastasis formation tumor cells need to migrate through cerebral endothelial cells, which form the morphological basis of blood-brain barrier (BBB). The mechanisms extravasation are highly uncharacterized, but in some aspects recapitulate diapedesis leukocytes. Extravasation leukocytes BBB is decreased by activation type 2 cannabinoid receptors (CB2); therefore, present study we sought investigate role CB2 interaction melanoma with endothelium. First, identified...

Liver fibrosis represents an overaccumulation of extracellular matrix (ECM). This study was designed to investigate the effect chrysin on established ECM overproduction in carbon tetrachloride (CCl4) mouse liver fibrosis. Experimental induced by intraperitoneal injection 2 mL/kg CCl4 twice a week, for 7 weeks. Mice were orally treated with 3 doses (5,7-dihydroxyflavone). For assessment spontaneous reversion fibrosis, CCl4-treated mice investigated after weeks recovery time. Silymarin used as...

Silymarin (Sy) shows limited water solubility and poor oral bioavailability. Water-soluble hydroxypropyl (HPBCD) randomly methylated (RAMEB) β-cyclodextrins were designed to enhance anti-fibrotic efficiency of silymarin in CCl4-induced liver fibrosis mice. Experimental was induced by intraperitoneal injection with 2 ml/kg CCl4 (20% v/v) twice a week, for 7 weeks. Mice orally treated 50 mg/kg Sy-HPBCD, Sy-RAMEB free silymarin. For assessment the spontaneous reversion fibrosis, animals...