A5059502959- Diabetes and associated disorders
- Pancreatic function and diabetes
- Diabetes Management and Research
- Genetics and Neurodevelopmental Disorders
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Zebrafish Biomedical Research Applications
- Gestational Diabetes Research and Management
- Thyroid Disorders and Treatments
- Pregnancy and preeclampsia studies
- Epigenetics and DNA Methylation
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- RNA modifications and cancer
- Silk-based biomaterials and applications
- Doctoral Education Challenges and Solutions
- Meta-analysis and systematic reviews
- Lipid Membrane Structure and Behavior
- RNA Research and Splicing
- Health and Medical Research Impacts
- Metabolism, Diabetes, and Cancer
- Folate and B Vitamins Research
- Birth, Development, and Health
Helmholtz Zentrum München
2024-2025
Karolinska Institutet
2015-2024
German Center for Diabetes Research
2024
Heinrich Heine University Düsseldorf
2024
Deutsches Diabetes-Zentrum e.V.
2024
Abstract Insulin binds the insulin receptor (IR) and regulates anabolic processes in target tissues. Impaired IR signalling is associated with multiple diseases, including diabetes, cancer neurodegenerative disorders. IRs have been reported to form nanoclusters at cell membrane several types, even absence of binding. Here we exploit nanoscale spatial organization achieve controlled multivalent activation. To control valency, developed rod-like insulin–DNA origami nanostructures carrying...
Abstract There is great interest in therapeutically harnessing endogenous regenerative mechanisms to increase the number of β cells people with diabetes. By performing whole‐genome expression profiling zebrafish islets, we identified 11 secreted proteins that are upregulated during β‐cell regeneration. We then tested proteins' ability potentiate regeneration at supraphysiological levels. One protein, insulin‐like growth factor (Igf) binding‐protein 1 (Igfbp1), potently promoted by...
Abstract Regenerating pancreatic β-cells is a potential curative approach for diabetes. We previously identified the small molecule CID661578 as potent inducer of β-cell regeneration, but its target and mechanism action have remained unknown. now screened 257 million yeast clones determined that targets MAP kinase-interacting serine/threonine kinase 2 (MNK2), an interaction we genetically validated in vivo. increased neogenesis from ductal cells zebrafish, neonatal pig islet aggregates human...
Primary human pancreatic ductal organoids (HPDO) have emerged as a model to study pancreas biology and disease. Yet, donor material availability, lack of extensive benchmarking limits the range applications. To address this gap, we established porcine (PPDO) system from an easily obtainable source ductal/progenitor biology. We benchmarked PPDO HPDO primary using single-cell RNA sequencing (scRNA-Seq). observed no overt phenotypic differences in derived distinct developmental stages, with WNT...
Abstract Diabetes can be caused by an insufficiency in β-cell mass. Here, we performed a genetic screen zebrafish model of loss to identify pathways promoting regeneration. We found that both folate receptor 1 ( folr1 ) overexpression and treatment with folinic acid, stimulated differentiation zebrafish. Treatment acid also cultures neonatal pig islets, showing the effect could translated mammalian system. In increased originated from ductal cells. Mechanistically, comparative metabolomic...
Building a blastema from the stump is key step of salamander limb regeneration. Stump-derived cells temporarily suspend their identity as they contribute to by process generally referred dedifferentiation. Here, we provide evidence for mechanism that involves an active inhibition protein synthesis during formation and growth. Relieving this results in higher number cycling enhances pace By small RNA profiling fate mapping skeletal muscle progeny cellular model dedifferentiation, find...
Introduction Inconsistent results have been reported on the association between folic acid use in pregnancy and risk of GDM. The aim this study was to estimate GDM two population-based Nordic cohorts. Material methods Two cohort studies were conducted using data from national population registers Norway (2005–2018, n = 791,709) Sweden (2006–2016, 1,112,817). Logistic regression used associations self-reported prescribed use, compared non-users, adjusting for covariates. To quantify how...
Abstract Regeneration of insulin-producing β-cells is an alternative avenue to manage diabetes, and it crucial unravel this process in vivo during physiological responses the lack β-cells. Here, we aimed characterize how hepatocytes can contribute β-cell regeneration a zebrafish model ablation. Using lineage-tracing, show that do not directly convert into even under extreme ablation conditions. A transcriptomics analysis isolated following displayed altered lipid- glucose-related processes....
Doctoral education is a critical mission of higher institutions (HEIs), and supervisors PhD students have prominent role in the process. The relevance their successful experience outcome raises an important question: how are trained for roles doctoral process? In this work, we aimed to identify formal training components syllabi supervision courses Swedish HEIs. Accordingly, used mixed methods approach thematic analysis uncover themes publicly available supervisor followed by quantification...
Regeneration of insulin-producing β-cells is an alternative avenue to manage diabetes, and it crucial unravel this process in vivo during physiological responses the lack β-cells. Here, we aimed characterize how hepatocytes can contribute β-cell regeneration, either directly or indirectly via secreted proteins metabolites, a zebrafish model loss. Using lineage tracing, show that do not convert into even under extreme ablation conditions. A transcriptomic analysis isolated after displayed...
Abstract DNA origami-based nanotechnology is a versatile tool for exploring fundamental biological questions and holds significant promise future biomedical applications. However, the development of therapeutic agents hindered by challenge translating in vitro performance into effective applications vivo . Here, we exploit optical transparency embryonic zebrafish to track intravenously injected, fluorescently labelled wireframe origami nanostructures. Our approach integrated long-term,...
Background Experimental animal studies suggest a novel role for the folate receptor 1 in β-cell differentiation pancreas, with potential implications glycemic control. We tested hypothesis of protective association between prenatal folic acid use and neonatal diabetes or hyperglycemia type an observational cohort study using data from national population health registers Norway. Methods All singleton pregnancies resulting live births 2005 to 2018 were identified. Prenatal exposure was...