A5002560238- Advancements in Photolithography Techniques
- bioluminescence and chemiluminescence research
- Advanced X-ray Imaging Techniques
- RNA Interference and Gene Delivery
- Particle Accelerators and Free-Electron Lasers
- Advanced biosensing and bioanalysis techniques
- Monoclonal and Polyclonal Antibodies Research
- 3D Printing in Biomedical Research
- Gyrotron and Vacuum Electronics Research
- Viral Infectious Diseases and Gene Expression in Insects
- Neuroscience and Neural Engineering
- Prion Diseases and Protein Misfolding
- T-cell and B-cell Immunology
- CRISPR and Genetic Engineering
- Infrared Thermography in Medicine
- Microfluidic and Bio-sensing Technologies
- Cell Image Analysis Techniques
- Advanced optical system design
- Particle accelerators and beam dynamics
- Surface Roughness and Optical Measurements
- Animal Ecology and Behavior Studies
- Brucella: diagnosis, epidemiology, treatment
- Advanced Surface Polishing Techniques
- Optical Coatings and Gratings
- Medical and Biological Ozone Research
Karolinska Institutet
2014-2024
École Polytechnique Fédérale de Lausanne
2015-2019
Russian Academy of Sciences
2008-2012
Budker Institute of Nuclear Physics
2007-2009
Abstract Insulin binds the insulin receptor (IR) and regulates anabolic processes in target tissues. Impaired IR signalling is associated with multiple diseases, including diabetes, cancer neurodegenerative disorders. IRs have been reported to form nanoclusters at cell membrane several types, even absence of binding. Here we exploit nanoscale spatial organization achieve controlled multivalent activation. To control valency, developed rod-like insulin–DNA origami nanostructures carrying...
Through the use of optical tweezers we performed controlled translocations DNA–protein complexes through nanocapillaries. We used RNA polymerase (RNAP) with two binding sites on a 7.2 kbp DNA fragment and dCas9 protein tailored to have five λ-DNA (48.5 kbp). Measured localization showed shift from expected positions that explained using both analytical fitting stochastic model. From measured force versus stage curves extracted nonequilibrium work done during translocation complex it obtain...
The capability to rapidly design proteins with novel functions will have a significant impact on medicine, biotechnology and synthetic biology. Synthetic genes are becoming commodity, but integrated approaches yet be developed that take full advantage of gene synthesis. We solid-phase synthesis method based asymmetric primer extension (APE) coupled this process directly high-throughput, on-chip protein expression, purification characterization (via mechanically induced trapping molecular...
Synthetically engineered cells are powerful and potentially useful biosensors, but it remains problematic to deploy such systems due practical difficulties biosafety concerns. To overcome these hurdles, we developed a microfluidic device that serves as an interface between cellular system, environment, user. We created biodisplay consisting of 768 individually programmable biopixels demonstrated can perform multiplexed, continuous sampling. The detected 10 μg/L sodium-arsenite in tap water...
ABSTRACT Autoimmune diseases, driven by Fcγ receptor (FcγR) activation through autoantibody immune complexes (IC), present a complex therapeutic challenge of achieving pharmacological blockade FcγR without triggering activation. The assembly ICs into polydisperse, higher-order structures is required for However, engineered multimeric, monodisperse Fc assemblies have been reported to prevent activation, suggesting that spatial organization determines In this study, we functional single-chain...
Application of dynamic X-ray lithography for originating continuous-relief diffractive optical elements and microoptics has been investigated. The method is based on action irradiation passed through fixed mask with triangle-like openings to periodically moved PMMA wafer. samples were exposed synchrotron radiation facility VEPP-3 (BINP SB RAS, Novosibirsk) then etched in liquid developer form surface relief. Advances limitations the have discussed. moving wafer fabrication gradient demonstrated.
In this paper we report about initiating technological development of thick metal mesh structures intended for frequency filtration high-power terahertz radiation Novosibirsk free electron laser. The technology is based on the promising LIGA-technique which employs effect high penetrability X-rays into roentgenosensitive resistive layers and destined manufacturing microstructures with extremely aspect ratios. Due to metallization thickness comparable wavelength LIGA-structures are expected...
The capability to rapidly design proteins with novel functions will have a significant impact on medicine, biotechnology, and synthetic biology. Synthetic genes are becoming commodity, but integrated approaches yet be developed that take full advantage of gene synthesis. We solid-phase synthesis method based asymmetric primer extension (APE) coupled this process directly high-throughput, on-chip protein expression, purification, characterization (mechanically induced trapping molecular...
Abstract Synthetically engineered cells are powerful and potentially useful biosensors, but it remains problematic to deploy such systems due practical difficulties biosafety concerns. To overcome these hurdles, we developed a microfluidic device that serves as an interface between cellular system, environment, user. We created biodisplay consisting of 768 individually programmable biopixels demonstrated can perform multiplexed, continuous sampling. The detected 10 µg/l sodium-arsenite in...