A5001805971- Immune Response and Inflammation
- Influenza Virus Research Studies
- Immunotherapy and Immune Responses
- Viral gastroenteritis research and epidemiology
- Monoclonal and Polyclonal Antibodies Research
- Animal Virus Infections Studies
- RNA and protein synthesis mechanisms
- Respiratory viral infections research
- Immune Cell Function and Interaction
- Bacteriophages and microbial interactions
- Malaria Research and Control
- Viral Infections and Immunology Research
- Advanced biosensing and bioanalysis techniques
- Virus-based gene therapy research
- T-cell and B-cell Immunology
- RNA Interference and Gene Delivery
- Cervical Cancer and HPV Research
- Hepatitis B Virus Studies
- vaccines and immunoinformatics approaches
- interferon and immune responses
- Herpesvirus Infections and Treatments
- Escherichia coli research studies
- Poxvirus research and outbreaks
- Cytokine Signaling Pathways and Interactions
- Pregnancy-related medical research
QB3
2020-2023
University of California, Berkeley
2020-2023
Karolinska Institutet
2012-2022
VaxInnate (United States)
2005-2013
Vedantra Pharmaceuticals (United States)
2012-2013
United States Military Academy
1993-2009
Philogen (Italy)
2009
Bayer (Italy)
2009
Istituto Giannina Gaslini
2009
European Institute of Oncology
2009
Advanced glycosylation end products of proteins (AGEs) are nonenzymatically glycosylated which accumulate in vascular tissue aging and at an accelerated rate diabetes. A approximately 35-kDa polypeptide with a unique NH2-terminal sequence has been isolated from bovine lung found to be present on the surface endothelial cells where it mediates binding AGEs (receptor for advanced product or RAGE). Using oligonucleotide probe based amino-terminal RAGE, apparently full-length cDNA 1.5 kilobases...
In the assembly of DNA nanostructures, specificity Watson-Crick base pairing is used to control matter at nanoscale. Using this technology for drug delivery a promising route toward magic bullet concept, as it would allow realization complex assemblies that co-localize drugs, targeting ligands and other functionalities in one nanostructure. Anthracyclines' mechanism action cancer therapy intercalate DNA, since nanotechnology allows such high degree customization, we hypothesized us tune...
The safety and immunogenecity of a booster dose live attenuated varicella-zoster virus (VZV) vaccine was evaluated in 196 healthy subjects, >or=60 years old, who had already received VZV >5 before. This repeat well tolerated. Cell-mediated immunity (CMI) to measured by an interferon-gamma (IFN-gamma) enzyme-linked immunosorbent spot-forming cell (ELISPOT) assay limiting dilution responder frequency (RCF) assay. Prevaccination responses decreased as function increasing age but were detectable...
The protein portion of the immunosuppressive glycoprotein uromodulin is identical to Tamm-Horsfall urinary and synthesized in kidney. Evidence that glycoproteins are same based on amino acid sequence identity, immunologic cross-reactivity, tissue localization thick ascending limb Henle's loop. Nucleic sequencing clones for isolated from a complementary DNA bank human kidney predicts 639 acids length, including 24--amino leader cysteine-rich mature with eight potential glycosylation sites....
U‐937 cells, a monocytic line derived from human histiocytic lymphoma, were induced for tumor necrosis factor (TNF) secretion into the medium and used preparation of TNF mRNA. Biological activity latter was quantified in Xenopus laevis oocyte injection system. mRNA enriched by gradient centrifugation this size‐fractionated synthesis cDNA inserted unique Pst I site pAT153. A recombinant plasmid containing selected colony hybridization using an internal fragment mouse clone [Fransen, L.,...
Thioredoxin is the best representative enzyme of a group proteins, widely distributed and possessing dithiol-disulfide oxidoreductase activity. We have constructed cDNA library from messenger RNAs isolated lymphoblastoid B cell line (Epstein-Barr virus-immortalized normal human lymphocytes). Screening this with synthetic oligonucleotide probes, NH2-terminal amino acid sequence protein produced by line, allowed us to identify full-length clone coding for thioredoxin. The open reading frame...
The antiviral characteristics of monoclonal antibody IAM-41-2F5 (2F5) were determined in cell culture. had been previously shown to bind a specific sequence, ELDKWA, within the external domain gp41 envelope glycoprotein human immunodeficiency virus type 1 (HIV-1). Selection by 2F5 recombinant phage from an epitope library confirmed identification antibody's binding determinant. was found be capable neutralizing broad range lymphoid culture-adapted HIV-1 variants as well primary isolates....
IL-1 plays an important role in the pathophysiologic responses to infection and inflammation, part by mediating its own production that of other proinflammatory cytokines. However, relative contribution alpha beta inflammatory response has not been well clarified. Using beta-deficient (IL-1 -/-) mice, we investigated specific vivo vitro LPS. No differences between +/+ -/- mice were observed circulating levels for alpha, IL-6, or TNF-alpha after systemic administration either a low (5...
The high‐level expression of human interleukin‐1β in Escherichia coli is described. protein contributes about 12% the total cell and associated with soluble cytoplasmic fraction cell. A method for purification given which based on anion‐ cation‐exchange chromatographies. isolated protein, shown to be homogeneous by several analytical methods, has been characterized amino acid analysis, N‐ C‐terminal sequence analysis centrifugation. biologically active as demonstrated two different vitro...
The high programmability of DNA origami has provided tools for precise manipulation matter at the nanoscale. This opens up possibility to arrange functional elements a diverse range applications that utilize nanometer precision by these structures. However, realization functionalized still suffers from imperfect production methods, in particular purification step, where excess material is separated desired origami. In this article we demonstrate and optimize two methods have not previously...
Production of interleukin 1 (IL-1) by leukemic cells was studied in 13 cases acute myeloid leukemia. Intracytoplasmic immunofluorescence studies showed that the invariably contained cytokine. Endogenous labeling demonstrated leukemia produced either only 33-kDa propeptide or both and 17-kDa mature form IL-1 beta. The alpha always but less frequently released. Involvement cell growth investigated using two antibodies specific for subtypes, which inhibited spontaneous proliferation six...