A5002287446- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- DNA and Nucleic Acid Chemistry
- Ubiquitin and proteasome pathways
- Cancer-related molecular mechanisms research
- Cancer-related gene regulation
- Protein Structure and Dynamics
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- NF-κB Signaling Pathways
- Enzyme Structure and Function
- Epigenetics and DNA Methylation
- Organometallic Compounds Synthesis and Characterization
- RNA Interference and Gene Delivery
- Cell Adhesion Molecules Research
- Chemical Synthesis and Characterization
- Glycosylation and Glycoproteins Research
- Protein Interaction Studies and Fluorescence Analysis
- Hemoglobin structure and function
- RNA regulation and disease
- Biopolymer Synthesis and Applications
- Organophosphorus compounds synthesis
- interferon and immune responses
- Mass Spectrometry Techniques and Applications
- Viral Infectious Diseases and Gene Expression in Insects
University of Trieste
2004-2018
University of London
2003
University College London
2003
University of Udine
2003
University of Reggio Calabria
1995
Weatherford College
1992
Institute of Cancer Research
1992
Portsmouth College
1981-1984
Elevated expression of the three high-mobility group I (HMGI) proteins (HMGI, HMGY, and HMGI-C) has previously been correlated with presence a highly malignant phenotype in epithelial fibroblastic rat thyroid cells experimental thyroid, lung, mammary, skin carcinomas. Northern (RNA) blot run-on analyses demonstrated that induction HMGI genes transformed occurs at transcriptional level. An antisense methodology to block HMGI-C protein synthesis was then used analyze role this process cell...
Journal Article cDNA cloning of the HML-C phosphoprotein, a nclear protein associated with neoplastic and undifferentiated phenotypes Get access G. Manfioletti, Manfioletti Search for other works by this author on: Oxford Academic PubMed Google Scholar V. Giancotti, Giancotti A. Bandiera, Bandiera E. Buratti, Buratti P. Sautièere, Sautièere 1Centre National de la Recherche Scientifique, Institute sur le Cancer59045, Lille, France Cary, Cary 2Biophysics Laboratories, Portsmouth...
This paper describes the characterisation of a novel chicken homeobox gene, Prh, whose encoded homeodomain sequence differs significantly from those other factors which have been described. As expected, portion protein, containing homeodomain, is capable sequence-specific DNA-binding. Outside possesses an N-terminal region extremely rich in proline residues and C-terminal acidic portion, either may function as transcription regulatory domains. Since, among tissues tested, its restricted to...
The HMGA2 protein belongs to the HMGA family of architectural transcription factors, which play an important role in chromatin organization. proteins are overexpressed several experimental and human tumors have been implicated process neoplastic transformation. Hmga2 knockout results pygmy phenotype mice a decreased growth rate embryonic fibroblasts, thus indicating for cell proliferation. Here we show that associates with E1A-regulated transcriptional repressor p120(E4F), interfering...
The nuclear magnetic resonance (NMR) spectrum of chromatin at ionic strengths below about 0.5 M may be attributed solely to its histone H1 component. effect various ions and urea on the complex has been investigated using NMR confirm that contraction increase strength is largely due electrostatic interactions. A detailed study · DNA also undertaken. behaviour in two cases virtually identical, implying complexed with rather than other histones. Microcalorimetric measurements reveal binding...
Abstract HMGA1 is an architectural transcription factor expressed at high levels in transformed cells and tumors. Several lines of evidence indicate that up-regulation involved the malignant transformation thyroid epithelial cells. However, mechanisms underlying effect on cancer cell phenotype are not fully understood. We now show cells, down-regulation by small interfering RNA antisense techniques results enhanced transcriptional activity p53, TAp63α, TAp73α, and, consequently, increased...
The HMGA1a protein belongs to the high mobility group A (HMGA) family of architectural nuclear factors, a proteins that plays an important role in chromatin dynamics. HMGA are multifunctional factors associate both with DNA and have been involved several processes, such as transcriptional regulation, viral integration, repair, RNA processing, remodeling. activity is finely modulated by variety post-translational modifications. Arginine methylation was recently demonstrated occur on protein,...
High mobility group A 1 (HMGA1) proteins are chromatinic factors, which absent or expressed at very low levels in normal adult tissues, while they over-expressed several human malignant tumors. In this study, HMGA1 protein expression was investigated by immunohistochemistry a series of 44 epithelial ovarian specimens, included four 29 primary invasive carcinomas, one metastatic tumor and 10 potential (LMP) staining not detected surface epithelium, is the area from adenocarcinoma frequently...
HMGI(Y) proteins are overexpressed in experimental and human malignancies, including colon, prostate thyroid carcinomas. To determine at which step of the carcinogenic process induction occurs, we analysed expression hyperplastic, preneoplastic neoplastic tissues colorectal origin by immunohistochemistry. All carcinomas were HMGI(Y)-positive, whereas no was detected normal colon mucosa tissue. adenomas closely correlated with degree cellular atypia. Only 2 18 non-neoplastic polyps tested...
Abstract We measured, by immunohistochemistry, HMGA1 protein expression in 212 breast tissue specimens: 6 normal samples, 28 hyperplastic lesions (13 with cellular atypia), 11 fibroadenomas, 10 situ ductal carcinomas, 144 and 13 lobular carcinomas. was not expressed tissue; staining intense 40% of atypia 60% carcinomas weak fibroadenomas without atypia. Because similar among different histologic grading, we evaluated the association between that other markers carcinoma invasion (estrogen...
The class I of the high mobility group (HMG) proteins is formed by phosphoproteins which are associated with AT‐rich DNA sequences in nucleus. Three HMGI have previously been described proliferating rodent cells (HMG Y, HMG and HMGI‐C). All three exhibit microheterogeneity. microheterogeneity mouse Y has investigated detail shown to be due phosphorylation protein sensitive alkaline‐phosphatase treatment. similarly modified. Human up now only found contain I. A search for third protein,...
Cancer is a very heterogeneous disease, and biological variability adds further level of complexity, thus limiting the ability to identify new genes involved in cancer development. Oncogenes whose expression levels control cell aggressiveness are useful for developing cellular models that permit differential screenings isogenic contexts. HMGA1 protein has this unique property because it master regulator breast cells transition from nontumorigenic epithelial-like phenotype toward highly...