A5080777829- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- NF-κB Signaling Pathways
- Cancer-related Molecular Pathways
- Cancer-related molecular mechanisms research
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Plant Molecular Biology Research
- RNA regulation and disease
- Epigenetics and DNA Methylation
- Peptidase Inhibition and Analysis
- Hemoglobinopathies and Related Disorders
- Plant Reproductive Biology
- interferon and immune responses
- RNA and protein synthesis mechanisms
- Sarcoma Diagnosis and Treatment
- Cancer-related gene regulation
- Signaling Pathways in Disease
- Histone Deacetylase Inhibitors Research
- Molecular Biology Techniques and Applications
- RNA Interference and Gene Delivery
- Heat shock proteins research
- Erythrocyte Function and Pathophysiology
- Protein Degradation and Inhibitors
- Particle physics theoretical and experimental studies
University of Milan
2015-2025
University of Modena and Reggio Emilia
1996-2023
University of Urbino
1993-2023
Museum of Science
2019
Centro di Riferimento Oncologico
2013
Istituto Nazionale di Fisica Nucleare, Sezione di Milano
2013
Mario Negri Institute for Pharmacological Research
2000-2009
PharmaMar (Spain)
2007
Université de Strasbourg
2003
Inserm
1991-2003
For many plant species, reproductive success relies on the proper timing of flowering, and photoperiod provides a key environmental input. Photoperiod-dependent flowering depends timely expression FLOWERING LOCUS T (FT); however, coordination various cis-regulatory elements in FT promoter is not well understood. Here, we provide evidence that long-distance chromatin loops bring distal enhancer into close association with proximal bound by CONSTANS (CO). Additionally, show NUCLEAR FACTOR Y...
NF-Y is a highly conserved heteromeric CCAAT-binding transcription factor involved in the function of several promoters.The NF-YA subunit contains domain high homology to yeast HAP2, which we show be necessary and sufficient mediate interactions with NF-YB DNA.Using protein affinity columns derivatized amino acid substitution mutants, further dissect this region into two functionally separable subdomains.The association resides 21-amino stretch, almost perfectly among different species,...
p63 is a transcription factor structurally related to the p53 tumor suppressor. The C-terminal region differs from p53's in that it contains sterile alpha motif (SAM) domain and subject multiple alternative splicings. N-terminal present transactivation (TA) ΔN configurations, with latter lacking transcriptional activation 1. Single amino acid substitutions frameshift mutations of cause human ankyloblepharon ectodermal dysplasia clefting (AEC) or ectrodactyly facial (EEC) syndromes. We have...
NF-Y is a trimeric activator with histone fold, HFM, subunits that binds to the CCAAT-box and required for majority of cell cycle promoters, often in conjunction E2Fs. In vivo binding dynamic during correlates gene activation. We performed immunofluorescence studies on endogenous, GFP- Flag-tagged overexpressed subunits. NF-YA, NF-YB are nuclear proteins. Unexpectedly, NF-YC localizes both cytoplamatic compartments its localization determined by interaction heterodimerization partner NF-YB....
In response to DNA damage, p53 activates G(1)/S blocking and apoptotic genes through sequence-specific binding. also represses with no target site, such as those for Cdc2 cyclin B, key regulators of the G(2)/M transition. Like most promoters, they rely on multiple CCAAT boxes activated by NF-Y, whose binding is temporally regulated during cell cycle. NF-Y associates in vitro vivo alphaC helix NF-YC (a subunit NF-Y) a region close tetramerization domain p53. Chromatin immunoprecipitation...
Ecteinascidin-743 (ET-743) is a tetrahydroisoquinoline alkaloid isolated from the tunicate Ecteinascidia turbinata currently under phase II clinical trials for its potent anticancer activity. ET-743 binds DNA in minor groove and forms covalent adducts with some sequence specificity. It selectively inhibits vitro binding of CCAAT box factor NF-Y. In this study, we assayed function vivo on HSP70 promoter. On heat induction, drug blocks transcription rapidly at pharmacological concentrations...
Np95 is an important determinant in cell cycle progression. Its expression tightly regulated and becomes detectable shortly before the entry of cells into S phase. Accordingly, absolutely required for G1/S transition. continued throughout S/G2/M phases further suggests additional roles. Indeed, has been implicated DNA damage response. Here, we show that bound to chromatin vivo it binds histones vitro. The binding direct shows a remarkable preference histone H3 its N-terminal tail. A novel...
Abstract Differentiation is a complex set of events that can be blocked by rearrangements regulatory genes producing fusion proteins with altered properties. In the case myxoid liposarcoma (MLS) tumors, causative abnormality between CHOP transcription factor and FUS or EWS genes. belongs to negative regulator large CAAT/enhancer binding protein family whose α, β,and δ members are master adipogenesis. Recent clinical data indicate peculiar sensitivity these tumors natural marine compound...
Nuclear Factor Y (NF-Y) is a heterotrimeric transcription factor that binds CCAAT elements. The NF-Y trimer composed of Histone Fold Domain (HFD) dimer (NF-YB/NF-YC) and NF-YA, which confers DNA sequence specificity. NF-YA shares conserved domain with the CONSTANS, CONSTANS-LIKE, TOC1 (CCT) proteins. We show CONSTANS (CO/B-BOX PROTEIN1 BBX1), master flowering regulator, forms Arabidopsis thaliana NF-YB2/NF-YC3 to efficiently bind CORE element FLOWERING LOCUS T promoter. term this complex...
NF-Y, a trimeric transcription factor (TF) composed of two histone-like subunits (NF-YB and NF-YC) sequence-specific subunit (NF-YA), binds to the CCAAT motif, common promoter element. Genome-wide mapping reveals 5000–15,000 NF-Y binding sites depending on cell type, with NF-YA NF-YB asymmetrically respect motif. Despite being characterized as proximal TF, only 25% map promoters. A comparable number are located at enhancers, many which tissue specific, nearly half in select subclasses HERV...
Rice flowering is controlled by changes in the photoperiod that promote transition to reproductive phase as days become shorter. Natural genetic variation for time has been largely documented and instrumental define genetics of photoperiodic pathway, well providing valuable material artificial selection varieties better adapted local environments. We mined a collection rice highly European regions isolated distinct variants long day repressor HEADING DATE 1 (Hd1) perturb its expression or...
During normal cell cycles, the function of mitotic cyclin-cdk1 complexes, as well cdc25C phosphatase, is required for G2 phase progression. Accordingly, arrest induced by DNA damage associated with a down-regulation cyclins, cdk1, and phosphatase expression. We found that promoter activity these genes repressed in damage. asked whether CCAAT-binding NF-Y modulates mitoticcyclins, gene transcription during this type arrest. In our experimental conditions, integrity CCAAT boxes ofcyclin B1,...
Journal Article Evolutionary variation of the CCAAT-binding transcription factor NF-Y Get access Xiao-Yan Li, Li Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 Biologie Moleculaire et Génie I'INSERM, Institut Chimie Biologique, Faculté MédecineStrasbourg, France Search for other works by this author on: Oxford Academic PubMed Google Scholar Roberto Mantovani, Mantovani Rob Hooft van Huijsduijnen, Huijsduijnen Isabelle Andre, Andre Christophe Benoist, Benoist * To whom...
NF-Y is a highly conserved transcription factor which recognizes CCAAT motifs in variety of genes.We report here the genomic organization genes encoding both subunits, gives interesting clues about functional proteins.We also existence isoforms NF-YA result from differential splicing.These alternative splicing events map within glutamine-rich activation domain and show marked cell-and tissue-specific bias.NF-Y eukaryotic that binds with high specificity to promoter regions transcribed by RNA...
Regulation of transcription during the cell-cycle is under control E2 factors (E2Fs), often in cooperation with nuclear factor Y (NF-Y), a histone-like CCAAT-binding trimer. NF-Y paradigmatic constitutive, ubiquitous that pre-sets promoter architecture for other regulatory proteins to access it. We analyzed recruitment NF-Y, E2F1/4/6, histone acetyltransferases, and deacetylase (HDAC) 1/3/4 several promoters by chromatin immunoprecipitation assays serum-starved restimulated NIH3T3 cells....