A5009822156- Cardiovascular Function and Risk Factors
- Nitric Oxide and Endothelin Effects
- Cardiac Ischemia and Reperfusion
- Cardiomyopathy and Myosin Studies
- Cardiac Fibrosis and Remodeling
- Advanced MRI Techniques and Applications
- Neuroscience and Neural Engineering
- Tissue Engineering and Regenerative Medicine
- Adipose Tissue and Metabolism
- Heart Rate Variability and Autonomic Control
- Renin-Angiotensin System Studies
- Mitochondrial Function and Pathology
- Cardiac Imaging and Diagnostics
- Congenital heart defects research
- Vagus Nerve Stimulation Research
- Advanced NMR Techniques and Applications
- Virus-based gene therapy research
- Cardiovascular Effects of Exercise
- Cardiovascular and exercise physiology
- Electron Spin Resonance Studies
- Cardiac electrophysiology and arrhythmias
- Heart Failure Treatment and Management
- Cardiac pacing and defibrillation studies
- Fuel Cells and Related Materials
- Cardiovascular Disease and Adiposity
Temple University
2016-2025
Scuola Superiore Sant'Anna
2016-2025
National Research Council
2007-2025
Istituto di Fisiologia Clinica
2007-2025
National Academies of Sciences, Engineering, and Medicine
2024-2025
Czech Academy of Sciences, Institute of Physiology
2000-2023
Fondazione Toscana Gabriele Monasterio
2010-2022
Cardiovascular Research Center
2015-2022
Institute of Life Sciences
2016-2021
Boston University
2018
Epidemiological studies suggest that Mediterranean diets rich in resveratrol are associated with reduced risk of coronary artery disease. Resveratrol was also shown to confer vasoprotection animal models type 2 diabetes and aging. However, the mechanisms by which exerts its antioxidative vasculoprotective effects not completely understood. Using a nuclear factor-E(2)-related factor-2 (Nrf2)/antioxidant response element-driven luciferase reporter gene assay, we found cultured arterial...
Myocardial infarction is a prevalent major cardiovascular event that arises from myocardial ischemia with or without reperfusion, and basic translational research needed to better understand its underlying mechanisms consequences for cardiac structure function. Ischemia underlies broad range of clinical scenarios ranging angina hibernation permanent occlusion, while reperfusion mandatory salvage ischemic injury, also inflicts injury on own. In this consensus statement, we present...
Evidence has emerged that the failing heart increases utilization of ketone bodies. We sought to determine whether this fuel shift is adaptive. Mice rendered incapable oxidizing body 3-hydroxybutyrate (3OHB) in exhibited worsened failure response fasting or a pressure overload/ischemic insult compared with WT controls. Increased delivery 3OHB ameliorated pathologic cardiac remodeling and dysfunction mice canine pacing model progressive failure. was shown enhance bioenergetic thermodynamics...
The nuclear receptors peroxisome proliferator-activated receptor-alpha (PPARalpha) and retinoid X receptor alpha (RXRalpha) stimulate the expression of key enzymes free fatty acid (FFA) oxidation. We tested hypothesis that altered metabolic phenotype failing heart involves changes in protein PPARalpha RXRalpha.Cardiac substrate uptake oxidation were measured 8 conscious, chronically instrumented dogs with decompensated pacing-induced failure normal by infusing 3 isotopically labeled...
Abstract —The aim of the present study was to determine whether cardiac nitric oxide (NO) production changes during progression pacing-induced heart failure and this occurs in association with alterations myocardial metabolism. Dogs (n=8) were instrumented paced until left ventricular end-diastolic pressure reached 25 mm Hg clinical signs severe evident. Every week, hemodynamic measurements recorded blood samples withdrawn from aorta coronary sinus for measurement NO metabolites, O 2...
Under resting conditions, the failing heart shifts fuel use toward greater glucose and lower free fatty acid (FFA) oxidation. We hypothesized that chronic metabolic abnormalities in patients with dilated cardiomyopathy (DCM) are associated absence of normal increase myocardial uptake maintenance cardiac mechanical efficiency response to pacing stress. In 10 DCM 6 control subjects, we measured coronary flow by intravascular ultrasonometry sampled arterial sinus blood. Myocardial metabolism...
Mounting evidence indicates that the function of members vascular endothelial growth factor (VEGF) family extends beyond blood vessel formation. Here, we show prolonged intramyocardial expression VEGF-A165 and VEGF-B167 on adeno-associated virus-mediated gene delivery determined a marked improvement in cardiac after myocardial infarction rats, by promoting contractility, preserving viable tissue, preventing remodeling left ventricle (LV) over time. Consistent with this functional outcome,...
Increased oxidative stress is a known cause of cardiac dysfunction in animals and patients with diabetes, but the sources reactive oxygen species [e.g., superoxide anion (O 2 − )] mechanisms underlying O production diabetic hearts are not clearly understood. Our aim was to determine whether NADPH oxidase (Nox) source glucose-6-phosphate dehydrogenase (G6PD)-derived plays role augmenting generation diabetes. We assessed function, Nox G6PD activities, levels, activities antioxidant enzymes...
We have developed a mixed ester of hyaluronan with butyric and retinoic acid (HBR) that acted as novel cardiogenic/vasculogenic agent in human mesenchymal stem cells isolated from bone marrow, dental pulp, fetal membranes term placenta (FMhMSCs). HBR remarkably enhanced vascular endothelial growth factor (VEGF), KDR, hepatocyte (HGF) gene expression the secretion angiogenic, mitogenic, antiapoptotic factors VEGF HGF, priming cell differentiation into cells. also increased transcription...
Hyperglycemia in diabetes mellitus promotes oxidative stress endothelial cells, which contributes to development of cardiovascular diseases. Nuclear factor erythroid 2-related factor-2 (Nrf2) is a transcription activated by that regulates expression numerous reactive oxygen species (ROS) detoxifying and antioxidant genes. This study was designed elucidate the homeostatic role adaptive induction Nrf2-driven free radical detoxification mechanisms protection under diabetic conditions. Using...
Hypopituitary Ames dwarf mice have low circulating growth hormone (GH)/IGF-I levels, and they extended longevity exhibit many symptoms of delayed aging. To elucidate the vascular consequences dwarfism we compared endothelial O 2 •− H production, mitochondrial reactive oxygen species (ROS) generation, expression antioxidant enzymes, nitric oxide (NO) production in aortas wild-type control mice. In ROS generation by mitochondria were enhanced with those vessels there was a less abundant...
Research Article27 May 2016Open Access Source DataTransparent process Follistatin-like 1 promotes cardiac fibroblast activation and protects the heart from rupture Sonomi Maruyama Department of Molecular Cardiology, Whitaker Cardiovascular Institute, Boston University School Medicine, Boston, MA, USA Search for more papers by this author Kazuto Nakamura Kyriakos N Papanicolaou Soichi Sano Ippei Shimizu Yasuhide Asaumi Maurice J van den Hoff Anatomy, Embryology Physiology, Academic Medical...
We have previously shown that VEGF165 gene delivery into ischemic skeletal muscle exerts not only proangiogenic, but also remarkable antiapoptotic and proregenerative activity. The aim of this study was to determine whether recombinant adeno-associated virus (rAAV)-mediated cardiac muscle, during acute myocardial infarction, a protective effect promote long-term functional recovery. Acute infarction the anterior LV wall induced in 12 chronically instrumented dogs by permanent occlusion LAD...
Vascular endothelial growth factor (VEGF)-B selectively binds VEGF receptor (VEGFR)-1, a that does not mediate angiogenesis, and is emerging as major cytoprotective factor.To test the hypothesis VEGF-B exerts non-angiogenesis-related cardioprotective effects in nonischemic dilated cardiomyopathy.AAV-9-carried VEGF-B(167) cDNA (10(12) genome copies) was injected into myocardium of chronically instrumented dogs developing tachypacing-induced cardiomyopathy. After 4 weeks pacing, green...
3,5,3′-Levo-triiodothyronine (L-T3) is essential for DNA transcription, mitochondrial biogenesis and respiration, but its circulating levels rapidly decrease after myocardial infarction (MI). The main aim of our study was to test whether an early sustained normalization L-T3 serum MI exerts protective effects through a preservation. Seventy-two hours induced by anterior interventricular artery ligation, rats were infused with synthetic (1.2 μg/kg/day) or saline over 4 weeks. Compared saline,...