A5005090268- Tissue Engineering and Regenerative Medicine
- Extracellular vesicles in disease
- Cardiovascular Function and Risk Factors
- Advanced MRI Techniques and Applications
- Advanced NMR Techniques and Applications
- Cardiac pacing and defibrillation studies
- Cardiac Fibrosis and Remodeling
- Congenital heart defects research
- Cardiac Ischemia and Reperfusion
- MicroRNA in disease regulation
- Electron Spin Resonance Studies
- Cardiac Structural Anomalies and Repair
- Nitric Oxide and Endothelin Effects
- Mesenchymal stem cell research
- Vagus Nerve Stimulation Research
- Cardiac Valve Diseases and Treatments
- Cardiac electrophysiology and arrhythmias
- Cardiovascular Disease and Adiposity
- Ultrasound and Hyperthermia Applications
- Neuroscience and Neural Engineering
- Heart Rate Variability and Autonomic Control
- Electrospun Nanofibers in Biomedical Applications
- Solid-state spectroscopy and crystallography
- COVID-19 Clinical Research Studies
- Cardiac Imaging and Diagnostics
Scuola Superiore Sant'Anna
2016-2025
Fondazione Toscana Gabriele Monasterio
2016-2025
Istituto di Fisiologia Clinica
2007-2024
University of Pisa
2007-2024
Azienda Ospedaliera Universitaria Senese
2024
Institute of Atmospheric Sciences and Climate
2022
Ludwig Boltzmann Institute for Cancer Research
2021
Ludwig Boltzmann Institute for Digital Health and Prevention
2021
National Research Council
2007-2018
Regione Toscana
2010-2018
AimsRecent evidence suggests that cardiac progenitor cells (CPCs) may improve function after injury. The underlying mechanisms are indirect, but their mediators remain unidentified. Exosomes and other secreted membrane vesicles, hereafter collectively referred to as extracellular vesicles (EVs), act paracrine signalling mediators. Here, we report EVs by human CPCs crucial cardioprotective agents.
Cell therapy trials using cardiac-resident progenitor cells (CPCs) and bone marrow-derived mesenchymal stem/progenitor (BMCs) in patients after myocardial infarction have provided encouraging results. Exosomes, nanosized extracellular vesicles of endosomal origin, figure prominently the bioactivities these cells. However, a head-to-head comparison exosomes from two cell types has not been performed yet. CPCs BMCs were derived cardiac atrial appendage specimens sternal marrow, respectively,...
Mounting evidence indicates that the function of members vascular endothelial growth factor (VEGF) family extends beyond blood vessel formation. Here, we show prolonged intramyocardial expression VEGF-A165 and VEGF-B167 on adeno-associated virus-mediated gene delivery determined a marked improvement in cardiac after myocardial infarction rats, by promoting contractility, preserving viable tissue, preventing remodeling left ventricle (LV) over time. Consistent with this functional outcome,...
We have developed a mixed ester of hyaluronan with butyric and retinoic acid (HBR) that acted as novel cardiogenic/vasculogenic agent in human mesenchymal stem cells isolated from bone marrow, dental pulp, fetal membranes term placenta (FMhMSCs). HBR remarkably enhanced vascular endothelial growth factor (VEGF), KDR, hepatocyte (HGF) gene expression the secretion angiogenic, mitogenic, antiapoptotic factors VEGF HGF, priming cell differentiation into cells. also increased transcription...
Aims: Sirtuins, a family of NAD+-dependent deacetylases, are recognized as nondispensable regulators aging processes. Sirtuin 3 (SIRT3) is the main mitochondrial deacetylase that maintains bioenergetics, an essential prerequisite for healthy aging. In this study, using Sirt3 knockout (Sirt3-/-) mice, we sought to establish whether deficiency affected life span, endpoint has never been tested formally in mammals, and uncover mechanisms involved organ damage associated with Results:Sirt3-/-...
Experimental evidence suggests that modulation of myocardial substrate metabolism can markedly affect the progression chronic heart failure (HF). We tested whether inhibition carnitine palmitoyl transferase-I (CPT-I), enzyme regulating mitochondrial fatty acid oxidation, slows left ventricular remodeling and deterioration function in pacing-induced HF.Normal dogs (n=9) were compared to untreated with HF treated 65 mg/kg/day oxfenicine (HF+Oxf, n=9), a CPT-I inhibitor.HF+Oxf reached terminal...
3,5,3′-Levo-triiodothyronine (L-T3) is essential for DNA transcription, mitochondrial biogenesis and respiration, but its circulating levels rapidly decrease after myocardial infarction (MI). The main aim of our study was to test whether an early sustained normalization L-T3 serum MI exerts protective effects through a preservation. Seventy-two hours induced by anterior interventricular artery ligation, rats were infused with synthetic (1.2 μg/kg/day) or saline over 4 weeks. Compared saline,...
Obesity and psychosocial stress (PS) co-exist in individuals of Western society. Nevertheless, how PS impacts cardiac hippocampal phenotype obese subjects is still unknown. Nor it clear whether changes local brain-derived neurotrophic factor (BDNF) account, at least part, for myocardial behavioral abnormalities experiencing PS.In adult male WT mice, obesity was induced via a high-fat diet (HFD). The resident-intruder paradigm superimposed to trigger PS. In vivo left ventricular (LV)...
The methods currently utilized to track stem cells by cardiac MRI are affected important limitations, and new solutions needed. We tested human ferritin heavy chain (hFTH) as a reporter gene for in vivo tracking of MRI. Swine stem/progenitor were transduced with lentiviral vector overexpress hFTH cultured obtain cardiospheres (Cs). Myocardial infarction was induced rats, and, after 45 min, the animals subjected intramyocardial injection ∼200 hFTH-Cs or nontransduced Cs saline solution border...
Glucose 6-phosphate dehydrogenase (G6PD) is the most common deficient enzyme in world. In failing hearts, G6PD upregulated and generates reduced nicotinamide adenine dinucleotide phosphate (NADPH) that used by glutathione pathway to remove reactive oxygen species but also as a substrate species-generating enzymes. Therefore, deficiency might prevent heart failure decreasing NADPH production.This hypothesis was evaluated mouse model of human (G6PDX mice, ≈40% normal activity). Myocardial...
Abstract Despite the widespread clinical use of cardioprotection by long-term direct antagonism P2Y12 receptor, underlying mechanisms are unclear. Here, we identify how release pro-survival exosomes from human cardiac-derived mesenchymal progenitor cells (hCPCs) is regulated clinically relevant dose ticagrelor (1 μM), an oral selective and reversible non-thienopyridine P2Y 12 inhibitor. Ticagrelor-induced enhancement exosome levels related to increased mitotic activity hCPCs. We show a...
Abstract Purpose During fall 2021, children’s hospitals in Germany faced a surge RSV-related hospitalizations, whereas during fall/winter 2022–2023, RSV and influenza infections both led to increased inpatient admissions. Our study prospectively assessed severe acute respiratory infections, their causative pathogens, the resulting disease burden on German children's for 2023–2024 season. Methods From October 3, 2023 through April 16, 2024, children hospitalized with ARI as primary diagnosis...
Myocardial infarction (MI) with resulting congestive heart failure is one of the leading causes death worldwide. Current therapies for treating MI, such as devices, traditional medicine, and surgeries, come many limitations patients in their final stages have little chances experiencing any reversible changes. In recent decades, Mesenchymal stem cell (MSC) based therapy has become most popular rapidly developing fields MI. Their supremacy clinical applications partially due to unique...