A5045411829- Cardiovascular Health and Disease Prevention
- Cardiovascular Disease and Adiposity
- Liver Disease Diagnosis and Treatment
- Cerebrovascular and Carotid Artery Diseases
- Ultrasound Imaging and Elastography
- Cardiovascular Function and Risk Factors
- Nanoplatforms for cancer theranostics
- Photoacoustic and Ultrasonic Imaging
- Blood Pressure and Hypertension Studies
- Cardiac Imaging and Diagnostics
- Cardiovascular Issues in Pregnancy
- Adipose Tissue and Metabolism
- Ultrasound and Hyperthermia Applications
- Non-Invasive Vital Sign Monitoring
- Optical Imaging and Spectroscopy Techniques
- Renal and Vascular Pathologies
- Heart Rate Variability and Autonomic Control
- Adenosine and Purinergic Signaling
- Coronary Interventions and Diagnostics
- Muscle metabolism and nutrition
- Coronary Artery Anomalies
- Retinal and Optic Conditions
- Systemic Sclerosis and Related Diseases
- Diet, Metabolism, and Disease
- Thermoregulation and physiological responses
Istituto di Fisiologia Clinica
2014-2023
Scuola Superiore Sant'Anna
2015-2023
National Research Council
2013-2023
Neuroscience Institute
2023
Czech Academy of Sciences, Institute of Physiology
2018-2019
MRC Unit for Lifelong Health and Ageing
2018
University College London
2018
National Research Council
2017
Manchester Metropolitan University
2016
Institute of Life Sciences
2015
Abstract Age-related cognitive impairment and dementia are an increasing societal burden. Epidemiological studies indicate that lifestyle factors, e.g. physical, social activities, correlate with reduced risk; moreover, positive effects on cognition of physical/cognitive training have been found in cognitively unimpaired elders. Less is known about effectiveness action mechanisms elders already suffering from Mild Cognitive Impairment (MCI), a population at high risk for dementia. We...
Abstract1. The activities of the ethoxycoumarin O-deethylase (ECOD), epoxide hydrolase (EH), UDP-glucuronyl transferase (GT), glutathione S-transferase (GST), acetyl (AT) and sulphotransferase (ST) were measured in 6 liver, 8 lung, kidney, intestinal mucosa 22 urinary bladder specimens from human subjects. EH GT studied with styrene oxide 1-naphthol, respectively, as substrates, GST, AT ST benzo(a)pyrene-4,5-oxide, p-aminobenzoic acid 2-naphthol, respectively.2. enzyme detectable at...
Obesity and psychosocial stress (PS) co-exist in individuals of Western society. Nevertheless, how PS impacts cardiac hippocampal phenotype obese subjects is still unknown. Nor it clear whether changes local brain-derived neurotrophic factor (BDNF) account, at least part, for myocardial behavioral abnormalities experiencing PS.In adult male WT mice, obesity was induced via a high-fat diet (HFD). The resident-intruder paradigm superimposed to trigger PS. In vivo left ventricular (LV)...
Basal expression of the P2X7 receptor (P2X7R) improves mitochondrial metabolism, Adenosine 5'-triphosphate (ATP) synthesis, and overall fitness immune non-immune cells. We investigated P2X7R contribution to energy metabolism subcellular localization in fibroblasts (mouse embryo HEK293 human fibroblasts), mouse microglia (primary brain microglia, N13 cell line), heart tissue. The localizes mitochondria, its lack (1) decreases basal respiratory rate, ATP-coupled respiration, maximal uncoupled...
Attenuated Listeria monocytogenes (Lmat-LLO) represents a valuable anticancer vaccine and drug delivery platform. Here we show that in vitro Lmat-LLO causes ROS production and, turn, apoptotic killing of wide variety melanoma cells, irrespectively their stage, mutational status, sensitivity to BRAF inhibitors or degree stemness. We also that, when administered the therapeutic setting Braf/Pten genetically engineered mice, strong decrease size volume primary tumors, as well reduction...
Abstract Background Exosomes (EXOs), tiny extracellular vesicles that facilitate cell–cell communication, are being explored as a heart failure treatment, although the features of cell source restrict their efficacy. Fibroblasts most prevalent non-myocyte cells, release poor cardioprotective EXOs. A noninvasive method for manufacturing fibroblast-derived exosomes (F-EXOs) target cardiomyocytes and slow cardiac remodeling is expected. As isothiocyanate, sulforaphane (SFN)-induced F-EXOs...
Photoacoustic imaging is an emerging technique. Although commercially available photoacoustic systems currently exist, the technology still in its infancy. Therefore, design of stable phantoms essential to achieve semiquantitative evaluation performance a system and can help optimize properties contrast agents. We designed developed polydimethylsiloxane (PDMS) phantom with exceptionally fine geometry; was tested using experiments loaded standard indocyanine green dye compared agar pattern...
Malignant melanoma (MM) is one of the most dangerous skin cancers. The aim this study was to present a potential new method for differential diagnosis MM from melanocytic naevi (MN). We examined 20 and 19 MN with ultra-high-frequency ultrasound (UHFUS) equipped 70 MHz linear probe. Ultrasonographic images were processed calculating 8 morphological parameters (area, perimeter, circularity, area ratio, standard deviation normalized radial range, roughness index, overlap ratio residual mean...
No treatment options are currently available to counteract cognitive deficits and/or delay progression towards dementia in older people with mild impairment (MCI). The 'Train the Brain' programme is a combined motor and intervention previously shown markedly improve functions MCI individuals compared non-trained controls, as assessed at end of 7-month intervention. Here, we extended previous analyses include long-term effects performed data disaggregation by gender, education age enrolled...
Arterial stiffness can be evaluated by calculating pulse wave velocity (PWV), i.e., the speed with which travels in a conduit vessel. This parameter is being increasingly investigated small rodent models it used for assessing alterations vascular function related to particular genotypes/treatments or characterizing cardiovascular disease progression. protocol describes an image processing algorithm leads non-invasive arterial PWV measurement mice using ultrasound (US) images only. The...
The main aim of this study is to evaluate the correspondence between ultrasonographic thickness and Breslow in melanoma using ultra-high frequency ultrasound intra- inter-operator repeatability measurements depth. Moreover, we propose a new protocol based on combined ultrasonographic-histopathological approach.We analyzed 27 melanomas population consisted patients (mean age 57.6 years, 51.8% males), who came at Department Dermatology (University Pisa, Italy) from April 2016 March 2018 had an...
Environmental enrichment may slow cognitive decay possibly acting through an improvement in vascular function. Aim of the study was to assess effects a 7-month cognitive, social, and physical training program on function patients with mild impairment. In single-center, randomized, parallel-group study, 113 (age, 65-89 years) were randomized multidomain (n=55) or usual care (n=58). All participants underwent neuropsychological tests evaluation, including brachial artery flow-mediated...
Central pulse pressure (cPP) is increasingly investigated as possible independent predictor of cardiovascular risk and carotid (carPP) can be used a surrogate cPP. Despite its importance, carPP measurement remains challenging in clinical practice. The aim this study was to introduce new easier-to-use method for noninvasive evaluation based on the use MEMS accelerometer.carPP values (carPP(acc)) were obtained 22 subjects (10 males, 47 ±17 years, hypertension: 50%) postprocessing double...
Abstract The ability of new polymeric materials to provide excellent biomechanical properties expanded their potential for biomedical applications enormously. use non‐invasive imaging modalities could crucial information monitor the efficacy/effectiveness/efficiency employed in ‘regenerative’ approaches, including scaffolds, hydrogels, self‐assembling and nanosized structures. assessment morpho‐functional metabolic changes treated or implanted tissues, visualization sites drug delivery...
Accurate dynamic three-dimensional (4D) imaging of the heart small rodents is required for preclinical study cardiac biomechanics and their modification under pathological conditions, but technological challenges are met in laboratory practice due to very size high pulse rate mice rats as compared humans. In 4D X-ray microtomography (4D μCT), achievable spatio-temporal resolution hampered by limitations conventional sources detectors. Here, we propose a proof-of-principle μCT platform,...
The availability of an animal model able to reliably mirror organ damage occurring in metabolic diseases is urgent need. These models, mostly rodents, have not been fully characterized terms cardiovascular, renal, and hepatic ultrasound parameters, only sparse values can be found literature. Aim this paper provide a detailed, noninvasive description the heart, vessels, liver, kidneys db/db mouse by imaging. Sixteen wild type thirty-four male mice (11-week-old) were studied. State-of-the-art...