A5047109750- Tissue Engineering and Regenerative Medicine
- Congenital heart defects research
- Mesenchymal stem cell research
- Electrospun Nanofibers in Biomedical Applications
- Congenital Heart Disease Studies
- Pluripotent Stem Cells Research
- Extracellular vesicles in disease
- Cardiac Structural Anomalies and Repair
- Cardiac Fibrosis and Remodeling
- Neonatal Respiratory Health Research
- Chemotherapy-induced cardiotoxicity and mitigation
- Cardiovascular Effects of Exercise
- MicroRNA in disease regulation
- Cellular Mechanics and Interactions
- CRISPR and Genetic Engineering
- PARP inhibition in cancer therapy
- Congenital Anomalies and Fetal Surgery
- Health and Medical Research Impacts
- Corneal Surgery and Treatments
- 3D Printing in Biomedical Research
- Pregnancy and preeclampsia studies
- Cardiomyopathy and Myosin Studies
- Organ and Tissue Transplantation Research
- Cardiovascular Function and Risk Factors
- Cardiac, Anesthesia and Surgical Outcomes
University of Genoa
2015-2024
Ospedale Policlinico San Martino
2015-2024
European Society of Cardiology
2020
John Wiley & Sons (United States)
2017
University of Oxford
2010-2017
Columbia Medical Practice
2017
University College London
2010-2016
March of Dimes
2016
Great Ormond Street Hospital
2011
University of Coimbra
2011
<h3>Objective</h3> Necrotising enterocolitis (NEC) remains one of the primary causes morbidity and mortality in neonates alternative strategies are needed. Stem cells have become a therapeutic option for other intestinal diseases, which share some features with NEC. We tested hypothesis that amniotic fluid stem (AFS) exerted beneficial effect neonatal rat model <h3>Design</h3> Rats intraperitoneally injected AFS their controls (bone marrow mesenchymal cells, myoblast) were analysed survival,...
Abstract Human amniotic fluid stem cells (hAFS) have shown a distinct secretory profile and significant regenerative potential in several preclinical models of disease. Nevertheless, little is known about the detailed characterization their secretome. Herein we show for first time that hAFS actively release extracellular vesicles (EV) endowed with paracrine effect. c-KIT+ were isolated from leftover samples prenatal screening stimulated to enhance EV (24 hours 20% O2 versus 1%...
In recent years, various types of stem cells have been characterized and their potential for cardiac regeneration has investigated. We previously described the isolation broadly multipotent from amniotic fluid, defined as fluid (AFS) cells. The aim this study was to investigate therapeutic human AFS (hAFS) in a model acute myocardial infarction. Wistar rats underwent 30 min ischemia by ligation left anterior descending coronary artery, followed administration hAFS 2 h reperfusion. Infarct...
Cardiovascular disease remains the major cause of mortality, and cardiac cell therapy has recently emerged as a paradigm for heart repair. The epicardium is layer mesothelial cells covering that during development contributes to different cardiovascular lineages, including cardiomyocytes, but which becomes quiescent after birth. We previously revealed peptide thymosin beta 4 (Tβ4) can reactivate adult epicardium-derived (EPDCs) myocardial infarction (MI), proliferate, differentiate into...
Epicardium-derived cells (EPDCs) contribute cardiovascular cell types during development and in adulthood respond to Thymosin β4 (Tβ4) myocardial infarction (MI) by reactivating a fetal gene programme promote neovascularization cardiomyogenesis. The mechanism for epicardial (re-)activation remains elusive. Here we reveal that BRG1, the essential ATPase subunit of SWI/SNF chromatin-remodelling complex, is required expression Wilms' tumour 1 (Wt1), EPDC activation subsequent differentiation...
Background Chronic cardiotoxicity is less common in male than female patients receiving doxorubicin and other anthracyclines at puberty adolescence. We hypothesized that this sex difference might be secondary to distinct activities of hormones on cardiomyocyte senescence, which thought central the development long‐term anthracycline cardiomyopathy. Methods Results H9c2 cells neonatal mouse cardiomyocytes were exposed with or without prior incubation testosterone 17β‐estradiol, main androgen...
Sphingolipids are structural components of cell membrane, displaying several functions in signalling. Extracellular vesicles (EV) lipid bilayer membrane nanoparticle and their composition may be different from parental cells, with a significant enrichment sphingolipid species, especially pathological conditions. We aimed at optimizing EV isolation plasma describing the differential content EV, as compared to whole plasma. As pilot study, we evaluated diagnostic potential lipidomic signature...
Long-term engraftment and phenotype correction has been difficult to achieve in humans after utero stem cell transplantation mainly because of allogeneic rejection. Autologous cells could be obtained during gestation from the amniotic fluid with minimal risk for fetus mother. Using a sheep model, we explored possibility using mesenchymal (AFMSCs) autologous cell/gene therapy. We collected (AF) under ultrasound-guided amniocentesis early pregnant (n = 9, 58 days gestation, term 145 days)....
Abstract The anthracycline doxorubicin (Dox) is widely used in oncology, but it may cause a cardiomyopathy with bleak prognosis that cannot be effectively prevented. secretome of human amniotic fluid-derived stem cells (hAFS) has previously been demonstrated to significantly reduce ischemic cardiac damage. Here shown that, following hypoxic preconditioning, hAFS conditioned medium (hAFS-CM) antagonizes senescence and apoptosis cardiomyocytes progenitor cells, two major features Dox...
Efficacy of adult (bone marrow, BM) versus fetal (amniotic fluid, AF) mesenchymal stem cells (MSCs) to replenish damaged rat heart tissues with new cardiovascular has not yet been established. We investigated on the differentiation potential these two MSC populations in vitro and a model acute necrotizing injury (ANI) induced by cryoinjury. Isolated BM-MSCs AF-MSCs were characterized flow cytometry cytocentrifugation their for osteogenic, adipogenic, assayed using specific induction media....