A5065744178- Liver Disease Diagnosis and Treatment
- Phagocytosis and Immune Regulation
- Liver Diseases and Immunity
- Anodic Oxide Films and Nanostructures
- Nuclear Receptors and Signaling
- Aortic aneurysm repair treatments
- RNA Interference and Gene Delivery
- Nanoparticle-Based Drug Delivery
- Cardiac Fibrosis and Remodeling
- MicroRNA in disease regulation
- Immune Cell Function and Interaction
- Genomics, phytochemicals, and oxidative stress
- Extracellular vesicles in disease
- Moyamoya disease diagnosis and treatment
- Cell Adhesion Molecules Research
- Electrospun Nanofibers in Biomedical Applications
- Immune cells in cancer
- Atherosclerosis and Cardiovascular Diseases
- Inhalation and Respiratory Drug Delivery
- Diabetes and associated disorders
- Diet, Metabolism, and Disease
- S100 Proteins and Annexins
- Graphene and Nanomaterials Applications
- Biomarkers in Disease Mechanisms
- Liver Disease and Transplantation
IRCCS Humanitas Research Hospital
2021-2024
Humanitas University
2017-2018
National Research Council
2016
Università degli Studi del Piemonte Orientale “Amedeo Avogadro”
2012-2015
Previous studies have shown that human nonalcoholic steatohepatitis (NASH) is often associated with the presence of circulating antibodies against protein adducted by lipid peroxidation products. Here we used methionine-choline deficient (MCD) model NASH to characterize possible involvement adaptive immunity in NASH. In mice fed up 8 weeks MCD diet extension liver injury and lobular inflammation paralleled development immunoglobulin G (IgG) malonyldialdehyde (MDA) 4-hydroxynonenal...
Inhalation delivers drug-loaded nanoparticles to the heart, improving cardiac function in murine and porcine models.
To develop biocompatible and bioresorbable negatively charged calcium phosphate nanoparticles (CaP-NPs) as an innovative therapeutic system for the delivery of bioactive molecules to heart.CaP-NPs were synthesized via a straightforward one-pot biomineralization-inspired protocol employing citrate stabilizing agent regulator crystal growth. CaP-NPs administered cardiac cells in vitro effects treatments assessed. vivo microRNAs was evaluated.CaP-NPs efficiently internalized into cardiomyocytes...
Annexin A1 (AnxA1) is an effector of the resolution inflammation and highly effective in terminating acute inflammatory responses. However, its role chronic settings less investigated. Because changes AnxA1 expression within adipose tissue characterize obesity mice humans, we queried a possible for pathogenesis nonalcoholic steatohepatitis (NASH), disease commonly associated with obesity. NASH was induced wild-type (WT) knockout (AnxA1 KO) C57BL/6 by feeding methionine-choline deficient...
Adult vascular smooth muscle cells (VSMCs) dedifferentiate in response to extracellular cues such as damage and inflammation. Dedifferentiated VSMCs are proliferative, migratory, less contractile, can contribute repair well cardiovascular pathologies intimal hyperplasia/restenosis coronary artery arterial aneurysm. We here demonstrate the role of ubiquitin-like containing PHD RING finger domains 1 (UHRF1) an epigenetic master regulator VSMC plasticity. UHRF1 expression correlated with...
Liver monocytes play a major role in the development of NASH (non-alcoholic steatohepatitis). In inflamed tissues, can differentiate both macrophages and dendritic cells. present study, we investigated moDCs (monocyte-derived inflammatory cells) experimental steatohepatitis induced C57BL/6 mice by feeding on MCD (methionine/choline-deficient) diet. The evolution was characterized an increase hepatic CD45+/CD11b+ myeloid cells displaying monocyte/macrophage marker F4-80+. early phases (4...
Increased shedding of extracellular vesicles (EVs)-small, lipid bilayer-delimited particles with a role in paracrine signalling-has been associated human pathologies, e.g. atherosclerosis, but whether this is true for cardiac diseases unknown.Here, we used the surface antigen CD172a as specific marker cardiomyocyte (CM)-derived EVs; CM origin CD172a+ EVs was supported by their content cardiac-specific proteins and heart-enriched microRNAs. We found that patients aortic stenosis, ischaemic...
Growing evidence indicates that NF-κB (nuclear factor κB) activation contributes to the pathogenesis of NASH (non-alcoholic steatohepatisis). Among subunits, p50/NF-κB1 has regulatory activities down-modulating NF-κB-mediated responses. In present study, we investigated effects NF-κB1 deficiency on progression induced by feeding mice an MCD (methionine/choline-deficient) diet. Following 4 weeks diet, steatosis, ALT (alanine aminotransferase) release, hepatocyte apoptosis, lobular...
BACKGROUND: Heart failure (HF) is strongly associated with inflammation. In pressure overload (PO)–induced HF, cardiac stress triggers adaptive immunity, ablation, or inhibition, which blocks disease progression. We hypothesized that PO-HF might fulfill the often-used criteria of autoimmunity: if so, immune response would be not only necessary but also sufficient to induce HF; it should possible identify self-antigens driving autoimmune response. Finally, we such an antigen-specific can...
The aim of our study was to set up a simple and reliable isolation method living ventricular cardiomyocytes (vCMs) for molecular biological studies.A standard technique the retrograde perfusion an enzymatic solution used isolate cardiac cells from adult mouse heart. Fluorescence-activated cell sorting (FACS) on murine ventricle performed, comparing intrinsic autofluorescence in FITC channel forward scatter (FSC) parameter order highly fluorescent cells. expression cell-specific mRNAs...