A5068225985- Epigenetics and DNA Methylation
- Nanoparticles: synthesis and applications
- Photoreceptor and optogenetics research
- Neuroscience and Neural Engineering
- Cell Adhesion Molecules Research
- Tissue Engineering and Regenerative Medicine
- Hydrogen's biological and therapeutic effects
- Cancer Research and Treatment
- RNA Research and Splicing
- Nuclear Structure and Function
- Cardiac electrophysiology and arrhythmias
- Electrohydrodynamics and Fluid Dynamics
- Photochromic and Fluorescence Chemistry
- Adenosine and Purinergic Signaling
- Cardiovascular Function and Risk Factors
- GDF15 and Related Biomarkers
- Genetics and Neurodevelopmental Disorders
- Medical and Biological Ozone Research
- Cardiac pacing and defibrillation studies
- Extracellular vesicles in disease
- Ion channel regulation and function
- Anesthesia and Neurotoxicity Research
- Cardiac Fibrosis and Remodeling
- Air Quality and Health Impacts
- Histone Deacetylase Inhibitors Research
IRCCS Humanitas Research Hospital
2021-2023
University of Parma
2015-2021
Humanitas University
2017-2020
Istituti di Ricovero e Cura a Carattere Scientifico
2020
National Research Council
2017
University of Verona
2015-2017
Imperial College London
2016
Mazzoni Center
2015
Correct gene expression programming of the cardiomyocyte underlies normal functioning heart. Alterations to this can lead loss cardiac homeostasis, triggering heart dysfunction. Although role some histone methyltransferases in establishing transcriptional program postnatal cardiomyocytes during development has been shown, function class epigenetic enzymes is largely unexplored adult In study, we investigated G9a/Ehmt2, a methyltransferase that defines repressive signature, defining for...
Increased shedding of extracellular vesicles (EVs)-small, lipid bilayer-delimited particles with a role in paracrine signalling-has been associated human pathologies, e.g. atherosclerosis, but whether this is true for cardiac diseases unknown.Here, we used the surface antigen CD172a as specific marker cardiomyocyte (CM)-derived EVs; CM origin CD172a+ EVs was supported by their content cardiac-specific proteins and heart-enriched microRNAs. We found that patients aortic stenosis, ischaemic...
Significance Pathological cardiac hypertrophy, characterized by heart growth in response to pressure or volume overload, such as the setting of hypertension, is main risk factor for failure (HF). The identification therapeutic strategies prevent reverse hypertrophy therefore a priority curing HF. It known that hormone-releasing hormone (GHRH) displays cardioprotective functions; however, its potential and HF unknown. Here we show GHRH reduces cardiomyocyte vitro through inhibition...
Non-communicable diseases, intended as the results of a combination inherited, environmental and biological factors, kill 40 million people each year, equivalent to roughly 70% all premature deaths globally. The possibility that manufactured nanoparticles (NPs) may affect cardiac performance, has led recognize NPs-exposure not only major Public Health concern, but also an occupational hazard. In volunteers, is problematic quantify. We recently found inhaled titanium dioxide NPs, one most...
Optical stimulation in the red/near infrared range recently gained increasing interest, as a not-invasive tool to control cardiac cell activity and repair disease conditions. Translation of this approach therapy is hampered by scarce efficacy selectivity. The use smart biocompatible materials, capable act local, NIR-sensitive interfaces with cells, may represent valuable solution, overcome these limitations. In work, far red-responsive conjugated polymer, namely...
The aim of our study was to set up a simple and reliable isolation method living ventricular cardiomyocytes (vCMs) for molecular biological studies.A standard technique the retrograde perfusion an enzymatic solution used isolate cardiac cells from adult mouse heart. Fluorescence-activated cell sorting (FACS) on murine ventricle performed, comparing intrinsic autofluorescence in FITC channel forward scatter (FSC) parameter order highly fluorescent cells. expression cell-specific mRNAs...
It has recently become evident that discrete clusters of L-type Ca2+ channels (LTCCs) exist along the cardiomyocyte sarcolemma in association with distinct membrane structures. Such microdomain-specific localization impact channel function and regulation by a variety neurohormonal pathways, including adrenergic adenosine. Disruption their subcellular targeting may contribute to pathophysiology cardiac diseases, heart failure (HF). We used imaging super-resolution scanning patch clamp examine...
Figure S3. TBARS measurement in lungs evaluated CTRL (white bars) and TiO2-NPs treated (black animals. Two-way ANOVA (post hoc analyses: Bonferroni test) was performed statistical significance set at p<0.05. ● vs CTRL; * corresponding 1° week; # 3° week. Data are represented as mean ± SEM. (PDF 60 kb)
Figure S2. TEM analysis of the alveolar lung parenchyma from a SHR rat seven weeks after intratracheal instillation TiO2-NPs. Individual or microaggregates small electrondense NPs are present in air space (As) as scattered within septum which type II pneumocyte (PII) is recognized. also apparent endothelial cells lining capillary (*) and lumen larger venule containing red blood (RBC) polymorphonuclear (PMN) neutrophils, lymphocytes (Lym) platelets (arrow). Magnification 1800X. Scale Bar:...
Figure S1. Spontaneous arrhythmic events. Different examples of Both, supraventricular (Supraventricular Extrasystole and Sinus Arrhythmia, SVA; Pause, SP) ventricular (Atrio-ventricular block, AV block; Ventricular Extrasystole, VE) events were recorded. (PDF 324 kb)