A5086237408- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Cardiovascular Effects of Exercise
- Tea Polyphenols and Effects
- Mitochondrial Function and Pathology
- Sirtuins and Resveratrol in Medicine
- Cardiovascular Function and Risk Factors
- Hydrogen's biological and therapeutic effects
- Signaling Pathways in Disease
- Cardiac electrophysiology and arrhythmias
- Nanoparticles: synthesis and applications
- Cardiomyopathy and Myosin Studies
- Cardiac Fibrosis and Remodeling
- Mesenchymal stem cell research
- Palliative Care and End-of-Life Issues
- Air Quality and Health Impacts
- Cancer Research and Treatment
- Pomegranate: compositions and health benefits
- Histone Deacetylase Inhibitors Research
- Pancreatic function and diabetes
- Heart Rate Variability and Autonomic Control
- Adipose Tissue and Metabolism
- Electrochemical Analysis and Applications
- Congenital heart defects research
- Cancer survivorship and care
University of Parma
2013-2022
University of Bologna
2014
University of Geneva
2003
Emerging evidence suggests that specific (poly)phenols may constitute new preventative strategies to counteract cell oxidative stress and myocardial tissue inflammation, which have a key role in the patho-physiology of diabetic cardiomyopathy. In rat model early diabetes, we evaluated whether vivo administration urolithin A (UA) or B (UB), main gut microbiota phenolic metabolites ellagitannin-rich foods, can reduce diabetes-induced microenvironmental changes tissue, preventing cardiac...
One of the most recently proposed candidates as a potential trigger for cardiovascular diseases is trimethylamine-N-oxide (TMAO). Possible direct effects TMAO on myocardial tissue, independent vascular damage, have been only partially explored so far. In present study, we assessed detrimental cardiomyocyte contractility and intracellular calcium dynamics, ability urolithin B-glucuronide (Uro B-gluc) in counteracting TMAO-induced cell damage. Cell mechanics transients were measured,...
In light of recent developments in nanotechnologies, interest is growing to better comprehend the interaction nanoparticles with body tissues, particular within cardiovascular system. Attention has recently focused on link between environmental pollution and diseases. Nanoparticles <50 nm size are known pass alveolar–pulmonary barrier, enter into bloodstream induce inflammation, but direct pathogenic mechanisms still need be evaluated. We thus our attention titanium dioxide (TiO2)...
Abstract We investigated the distribution of α‐skeletal, α‐cardiac, and α‐smooth muscle actin isoforms in human heart during development, hypertrophy, failure. At 20 weeks fetal life, α‐skeletal was localized a small proportion subendocardial papillary cardiomyocytes. this gestation time, diffuse α‐cardiac staining observed, associated with focal expression actin. In normal adult subjects, positive cardiomyocytes were distributed transmural gradient highest located subendocardially....
Emerging evidence suggests that both adult cardiac cell and the stem/progenitor (CSPC) compartments are involved in patho-physiology of diabetic cardiomyopathy (DCM). We evaluated whether early administration Resveratrol, a natural antioxidant polyphenolic compound, addition to improving cardiomyocyte function, exerts protective role on (i) progenitor pool, (ii) myocardial environment its impact CSPCs, positively interfering with onset DCM phenotype. Adult Wistar rats (n = 128)...
Non-communicable diseases, intended as the results of a combination inherited, environmental and biological factors, kill 40 million people each year, equivalent to roughly 70% all premature deaths globally. The possibility that manufactured nanoparticles (NPs) may affect cardiac performance, has led recognize NPs-exposure not only major Public Health concern, but also an occupational hazard. In volunteers, is problematic quantify. We recently found inhaled titanium dioxide NPs, one most...
The effect of histone deacetylase inhibitors on dystrophic heart function is not established. To investigate this aspect, mdx mice and wild-type (WT) animals were treated 90 days either with suberoylanilide hydroxamic acid (SAHA, 5 mg/kg/day) or an equivalent amount vehicle. following parameters evaluated: (i) number ventricular arrhythmias in resting stress conditions (restraint test) after aconitine administration; (ii) cardiac excitability, conduction velocity, refractoriness; (iii)...
One of the main cause ineffective cell therapy in repairing damaged heart is poor yield grafted cells. To overcome this drawback, rats with 4-week-old myocardial infarction (MI) were injected border zone human adipose-derived stem cells (ADSCs) conveyed by poly(lactic-co-glycolic acid) microcarriers (PAMs) releasing hepatocyte growth factor (HGF) and insulin-like factor-1 (IGF-1) (GFsPAMs). According to treatments, animals subdivided into different groups: MI_ADSC, MI_ADSC/PAM, MI_GFsPAM,...
Abstract Background Nanotoxicology is an increasingly relevant field and sound paradigms on how inhaled nanoparticles (NPs) interact with organs at the cellular level, causing harmful conditions, have yet to be established. This particularly true in case of cardiovascular system, where experimental clinical evidence shows morphological functional damage associated NP exposure. Giving increasing interest cobalt oxide (Co 3 O 4 ) NPs applications industrial bio-medical fields, a detailed...
Heart repair by stem cell treatment may involve life-threatening arrhythmias. Cardiac progenitor cells (CPCs) appear best suited for reconstituting lost myocardium without posing arrhythmic risks, being commissioned towards cardiac phenotype. In this study we tested the hypothesis that mobilization of CPCs through locally delivered Hepatocyte Growth Factor and Insulin-Like Factor-1 to heal chronic myocardial infarction (MI), lowers proneness We used 133 adult male Wistar rats either with...
c-Kit(pos) cardiac progenitor cells (CPCs) represent a successful approach in healing the infarcted heart and rescuing its mechanical function, but electrophysiological consequences are uncertain. CPC mobilization promoted by hepatocyte growth factor (HGF) IGF-1 improved electrogenesis myocardial infarction (MI). We hypothesized that locally delivered CPCs supplemented with HGF + (GFs) can concur ameliorating electrical stability of regenerated heart. Adult male Wistar rats (139 rats)...
SERCA2a is the Ca2+ ATPase playing major contribution in cardiomyocyte (CM) calcium removal. Its activity can be regulated by both modulatory proteins and several post-translational modifications. The aim of present work was to investigate whether function SERCA2 modulated treating CMs with histone deacetylase (HDAC) inhibitor suberanilohydroxamic acid (SAHA). incubation SAHA (2.5 µM, 90 min) isolated from rat adult hearts resulted an increase acetylation level improved activity. This...
In a rat model of long-lasting pressure-overload hypertrophy, we investigated whether changes in the relative expression myocardial actin isoforms are among early signs ventricular mechanical dysfunction before transition toward decompensation. Forty-four rats with infrarenal aortic banding (AC rats) were studied. Hemodynamic parameters measured 1 mo (AC(1) group; n = 20) or 2 (AC(2); 24) after ligature. Then subgroups AC(1) and AC(2) left ventricles (LV) used to evaluate 1) LV anatomy...
In early diabetes, hyperglycemia and the associated metabolic dysregulation promote changes in functional properties of cardiomyocytes, progressively leading to appearance diabetic cardiomyopathy phenotype. Recently, interplay between histone acetyltransferases (HAT) deacetylases (HDAC) has emerged as a crucial factor development cardiac disorders. The present study evaluates whether HDAC inhibition can prevent cardiomyocyte contractile dysfunction induced by short period hyperglycemia, with...
Background: Little information is currently available concerning the relative contribution of cardiac parenchymal and stromal cells in activation pro-inflammatory signal cascade, at initial stages diabetes. Similarly, effects early resveratrol (RSV) treatment on negative impact diabetes different myocardial cell compartments remain to be defined. Methods: In vitro challenge neonatal cardiomyocytes fibroblasts high glucose vivo/ex vivo experiments a rat model Streptozotocin-induced were used...
Background. Green tea catechins are known to promote mitochondrial function, and modulate gene expression signalling pathways that altered in the diabetic heart. We thus evaluated effectiveness of vivo administration a standardized green extract (GTE) restoring cardiac performance, rat model early streptozotocin-induced diabetes, with focus on underlying mechanisms. Methods. Twenty-five male adult Wistar rats were studied: control (n = 9), untreated animals 7) subjected daily GTE for 28 days...
Dietary polyphenols from green tea have been shown to possess cardio-protective activities in different experimental models of heart diseases and age-related ventricular dysfunction. The present study was aimed at evaluating whether long term vivo administration extracts (GTE), can exert positive effects on the normal heart, with focus underlying mechanisms.The population consisted 20 male adult Wistar rats. Ten animals were given 40 mL/day tap water solution GTE (concentration 0.3%) for 4...
We recently showed that the long-term in vivo administration of green tea catechin extract (GTE) resulted hyperdynamic cardiomyocyte contractility. The present study investigates mechanisms underlying GTE action comparison to its major component, epigallocatechin-3-gallate (EGCG), given at equivalent amount would be entirety GTE. Twenty-six male Wistar rats were 40 mL/day a tap water solution with either standardized or pure EGCG for 4 weeks. Cardiomyocytes then isolated study. Cellular...
This study investigated epigenetic risk factors that may contribute to stress-related cardiac disease in a rodent model. Experiment 1 was designed evaluate the expression of microRNA-34a (miR-34a), known modulator both stress responses and pathophysiology, heart male adult rats exposed single or repeated episodes social defeat stress. Moreover, RNA sequencing conducted identify transcriptomic profile changes repeatedly stressed rats. 2 assess electromechanical induced by predispose...
In normal rats, we analysed the arrhythmogenic role of intrinsic action potential duration (APD) heterogeneity. each animal, ventricular arrhythmic events (VAEs) occurring spontaneously and during exposure to an acute social challenge were telemetrically recorded. Action potentials recorded from isolated left myocytes, at a pacing rate 5 Hz (patch clamp: current-clamp mode). APDs measured -20 mV, -30 -40 -50 mV -60 mV. The difference between shortest longest APD was also computed, as index...