A5069986334- Ovarian cancer diagnosis and treatment
- Cancer Genomics and Diagnostics
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Genetic factors in colorectal cancer
- Circular RNAs in diseases
- Sarcoma Diagnosis and Treatment
- RNA modifications and cancer
- Peptidase Inhibition and Analysis
- RNA Research and Splicing
- Phagocytosis and Immune Regulation
- Cancer-related Molecular Pathways
- Ferroptosis and cancer prognosis
- Protein Degradation and Inhibitors
- Occupational and environmental lung diseases
- Molecular Biology Techniques and Applications
- Cancer Research and Treatments
- Cancer therapeutics and mechanisms
- Peroxisome Proliferator-Activated Receptors
- Cancer Cells and Metastasis
- Immune cells in cancer
- PARP inhibition in cancer therapy
- Cancer-related gene regulation
- interferon and immune responses
- FOXO transcription factor regulation
IRCCS Humanitas Research Hospital
2021-2025
Humanitas University
2021-2024
Mario Negri Institute for Pharmacological Research
2014-2023
Istituti di Ricovero e Cura a Carattere Scientifico
2014-2023
KU Leuven
2017
University of Milan
2000-2014
Fondazione Filarete
2014
National Institutes of Health
2014
National Cancer Institute
2014
University of Milano-Bicocca
2007-2013
Ovarian cancer is a leading cause of deaths among women. Effective targets to treat advanced epithelial ovarian (EOC) and biomarkers predict treatment response are still lacking because the complexity pathways involved in progression. Here we show that miR-181a promotes TGF-β-mediated epithelial-to-mesenchymal transition via repression its functional target, Smad7. phosphorylated Smad2 enriched recurrent compared with matched-primary tumours their expression associated shorter time...
Ecteinascidin-743 (ET-743) is a tetrahydroisoquinoline alkaloid isolated from the tunicate Ecteinascidia turbinata currently under phase II clinical trials for its potent anticancer activity. ET-743 binds DNA in minor groove and forms covalent adducts with some sequence specificity. It selectively inhibits vitro binding of CCAAT box factor NF-Y. In this study, we assayed function vivo on HSP70 promoter. On heat induction, drug blocks transcription rapidly at pharmacological concentrations...
Lurbinectedin is a novel anticancer agent currently undergoing late-stage (Phase II /III) clinical evaluation in platinum-resistant ovarian, BRCA1/2-mutated breast and small-cell lung cancer. structurally related to trabectedin it inhibits active transcription the DNA repair machinery tumour cells.In this study we investigated whether lurbinectedin has ability modulate inflammatory microenvironment viability of myeloid cells tumour-bearing mice.Administration significantly selectively...
Epilepsy therapy is based on drugs that treat the symptoms rather than underlying mechanisms of disease (epileptogenesis). There are no treatments for preventing seizures or improving prognosis, including neurological comorbidities. The search pathogenic epileptogenesis highlighted neuroinflammatory cytokines [i.e. interleukin-1β (IL-1β), tumour necrosis factor-α (Tnf-α)] induced in human and experimental epilepsies, contribute to seizure generation animal models. A major role controlling...
Abstract Aberrant microRNA (miR) expression has an important role in tumour progression, but its involvement bone marrow fibroblasts of multiple myeloma patients remains undefined. We demonstrate that a specific miR profile parallels the transition from monoclonal gammopathy undetermined significance (MGUS) to myeloma. Overexpression miR‐27b‐3p and miR‐214‐3p triggers proliferation apoptosis resistance via FBXW7 PTEN/AKT/GSK3 pathways, respectively. Transient transfection inhibitors...
Abstract Patients with colorectal liver metastasis (CLM) present heterogenous clinical outcomes and improved classification is needed to ameliorate the therapeutic output. Macrophages (Mϕ) hold promise as prognostic classifiers targets. Here, stemming from a single-cell analysis of mononuclear phagocytes infiltrating human CLM, we identified two Mϕ markers associated distinct populations opposite relevance. The invasive margin CLM was enriched in pro-inflammatory monocyte-derived (MoMϕ)...
Ovarian cancer (OvC) constitutes significant management challenges primarily due to its late-stage diagnosis and the development of resistance chemotherapy. The standard treatment regimen typically includes carboplatin paclitaxel, with addition poly (ADP-ribose) polymerase inhibitors for patients high-grade serous ovarian (HGSOC) harboring BRCA1/2 mutations. However, variability in responses suggests need investigate factors beyond mutations, such as DNA repair mechanisms epigenetic...
To define the features of human amniotic mesenchymal stromal cell secretome and its protective properties in experimental models acute brain injury.Prospective study.Laboratory research.C57Bl/6 mice.Mice subjected to sham or traumatic injury by controlled cortical impact received cells phosphate-buffered saline infused intracerebroventricularly intravenously 24 hours after injury. Organotypic slices exposed ischemic oxygen-glucose deprivation were treated with their (conditioned medium) a...
Purpose: Stage I epithelial ovarian cancer (EOC) represents about 10% of all EOCs and is characterized by good prognosis with fewer than 20% patients relapsing. As it occurs less frequently advanced-stage EOC, its molecular features have not been thoroughly investigated. We demonstrated that in stage EOC miR-200c-3p can predict patients' outcome. In the present study, we analyzed expression long non-coding RNAs (lncRNA) to enable potential definition a transcriptional signature prognostic...
Epithelial ovarian cancer (EOC) is a spectrum of different diseases, which makes their treatment challenge. Forkhead box M1 (FOXM1) an oncogene aberrantly expressed in many solid cancers including serous EOC, but its role non-serous EOCs remains undefined. We examined FOXM1 expression and correlation to prognosis across the three major EOC subtypes, tumorigenesis chemo-resistance vitro.Gene signatures were generated by microarray for 14 clear-cell 26 endometrioid EOCs, 15 normal endometrium...
Physical activity improves the prognosis of cancer patients, partly by contrasting associated muscle wasting (cachexia), through still unknown mechanisms. We asked whether aerobic exercise causes secretion skeletal muscles proteins (myokines) that may contrast cachexia. Media conditioned peroxisome proliferator-activated receptor γ coactivator 1α (PGC1α)-expressing myotubes, reproducing some metabolic adaptations exercise, as increased mitochondrial biogenesis and oxidative phosphorylation,...
Survival rates of oral squamous cell carcinoma (OSCC) remained substantially unchanged over the last decades; thus, additional prognostic tools are strongly needed.Salivary miRNAs have emerged as excellent non-invasive cancer biomarker candidates, but their association with OSCC prognosis has not been investigated yet.In this study, we analyzed global salivary miRNA expression in patients and healthy controls, aim to define its diagnostic potential.Methods: Saliva was collected from newly...
Epithelial ovarian cancer (EOC) is one of the most lethal gynecologic diseases, with survival rate virtually unchanged for past 30 years. EOC comprises different histotypes molecular and clinical heterogeneity, but up till now present gold standard platinum-based treatment has been conducted without any patient stratification. The aim study to generate microRNA (miRNA) profiles characteristic each stage I histotype, identify subtype-specific biomarkers improve our understanding underlying...