A5021860051- Renal Transplantation Outcomes and Treatments
- Cancer-related molecular mechanisms research
- Transplantation: Methods and Outcomes
- Ovarian cancer diagnosis and treatment
- Cancer Cells and Metastasis
- FOXO transcription factor regulation
- Viral-associated cancers and disorders
- Pregnancy and Medication Impact
- Polyomavirus and related diseases
- Monoclonal and Polyclonal Antibodies Research
- MicroRNA in disease regulation
- Renal and Vascular Pathologies
- Organ Transplantation Techniques and Outcomes
- Reproductive System and Pregnancy
- Cell Adhesion Molecules Research
- Cardiac Imaging and Diagnostics
- Barrier Structure and Function Studies
- Organ Donation and Transplantation
- Dialysis and Renal Disease Management
- RNA Research and Splicing
- Molecular Biology Techniques and Applications
- Bladder and Urothelial Cancer Treatments
- Endometrial and Cervical Cancer Treatments
- Metabolism and Genetic Disorders
- Cancer Genomics and Diagnostics
University of Bologna
2004-2023
Vita-Salute San Raffaele University
2011-2023
Université Paris Cité
2023
Institute Mutualiste Montsouris
2023
Puigvert Foundation
2023
Centre Hospitalier Universitaire de Nantes
2023
Policlinico S.Orsola-Malpighi
2004-2022
University of Brescia
2012-2021
Azienda Socio Sanitaria Territoriale degli Spedali Civili di Brescia
2018-2021
Institute of Molecular Medicine
2019
Uterine serous carcinoma (USC) is a biologically aggressive subtype of endometrial cancer. We analyzed the mutational landscape USC by whole-exome sequencing 57 cancers, most which were matched to normal DNA from same patients. The distribution number protein-altering somatic mutations revealed that 52 tumors had fewer than 100 (median 36), whereas 5 more 3,000 mutations. in these latter showed hallmarks defects mismatch repair. Among remainder, we found significantly increased burden...
Significance Some cancers, termed carcinosarcomas (CSs), have mixed cell types, with either epithelial or mesenchymal features. Sequencing the genomes of uterine and ovarian CSs demonstrated that these different types derive from a common precursor has many mutations typical cancers. In addition, we find tumors significant burden point amplification histone genes, suggesting potential role in sarcomatous transformation. Consistent this finding, expression specific gene carcinoma cells...
To date, no good marker for screening or disease monitoring of endometrial cancer (EC) is available. The aims this study were to investigate HE4 gene, protein expression and serum (sHE4) levels in a panel ECs normal endometria (NEs) correlate sHE4 with patient clinicopathological characteristics prognosis.Using quantitative real-time PCR we tested 46 20 NEs gene expression. Protein was analysed by immunohistochemistry on tissue microarrays 153 33 NEs. Pre-operative samples from 138 EC 76 NE...
Uterine leiomyosarcomas (uLMS) are aggressive tumors arising from the smooth muscle layer of uterus. We analyzed 83 uLMS sample genetics, including 56 Yale and 27 The Cancer Genome Atlas (TCGA). Among them, a total 55 samples two patient-derived xenografts (PDXs) TCGA have whole-exome sequencing (WES) data; 10 RNA-sequencing (RNA-Seq) 11 whole-genome (WGS) data. found recurrent somatic mutations in TP53, MED12, PTEN genes. Top mutated genes included ATRX, PTEN, MEN1 Somatic copy number...
Significance Ovarian cancer has the propensity for early dissemination of microscopic metastases, making detection challenging. Development treatments patients with advanced/recurrent chemotherapy-resistant disease remains an unmet need. We sequenced primary, synchronous bilateral ovarian (SBOC), metastatic, and recurrent tumors. found primary metastatic tumors to be remarkably similar SBOC clonally related in all cases, suggesting represent intrinsic feature cancer. c-MYC PIK3CA...
The prognosis of advanced/recurrent cervical cancer patients remains poor. We analyzed 54 fresh-frozen and 15 primary cell lines, along with matched-normal DNA, by whole-exome sequencing (WES), most which harboring Human-Papillomavirus-type-16/18. found recurrent somatic missense mutations in 22 genes (including PIK3CA, ERBB2, GNAS) a widespread APOBEC cytidine deaminase mutagenesis pattern (TCW motif) both adenocarcinoma (ACC) squamous carcinomas (SCCs). Somatic copy number variants (CNVs)...
Epithelial ovarian cancer (EOC) is a spectrum of different diseases, which makes their treatment challenge. Forkhead box M1 (FOXM1) an oncogene aberrantly expressed in many solid cancers including serous EOC, but its role non-serous EOCs remains undefined. We examined FOXM1 expression and correlation to prognosis across the three major EOC subtypes, tumorigenesis chemo-resistance vitro.Gene signatures were generated by microarray for 14 clear-cell 26 endometrioid EOCs, 15 normal endometrium...
Abstract BACKGROUND: Emerging evidence has suggested that the capability to sustain tumor formation, growth, and chemotherapy resistance in ovarian as well other human malignancies exclusively resides a small proportion of cells termed cancer stem cells. During characterization CD44 + cells, we found high expression genes encoding for claudin‐4. Because this tight junction protein is natural high‐affinity receptor Clostridium perfringens enterotoxin (CPE), have extensively investigated...
Endometrial cancer is the most common gynecologic malignancy in developed countries. Trop-2 a glycoprotein involved cellular signal transduction and differentially overexpressed relative to normal tissue variety of human adenocarcinomas, including endometrioid endometrial carcinomas (EEC). overexpression has been proposed as marker for biologically aggressive tumor phenotypes.Trop-2 protein expression was quantified using microarrays consisting formalin-fixed paraffin-embedded specimens from...
Abstract Micro RNA s (mi s) belong to a family of small non‐coding (snc playing important roles in human carcinogenesis. Multiple investigations reported mi aberrantly expressed several cancers, including high‐grade serous ovarian carcinoma ( HGS ‐OvCa). Quantitative PCR is widely used studies investigating expression and the identification reliable endogenous controls crucial for proper data normalization. In this study, we aimed experimentally identify most stable reference snc...
Kidney transplantation (KT) may restore fertility in chronic kidney disease (CKD). The reasons why maternofetal outcomes are still inferior to the overall population only partially known. Comparison with CKD offer some useful insights for management and counselling.Aim of this study was analyse pregnancy after KT, compared a large nontransplanted patients low-risk control pregnancies, observed Italy new millennium.We selected 121 live-born singletons KT (Italian group pregnancy, national...
Abstract BACKGROUND: Uterine serous papillary carcinoma (USPC) was an aggressive and chemotherapy resistant variant of endometrial cancer. The authors evaluated the expression human trophoblast‐cell‐surface‐marker (Trop‐2) potential hRS7, a humanized anti‐Trop‐2 monoclonal antibody, as novel therapeutic strategy against USPC. METHODS: Trop‐2 by immunohistochemistry (IHC) in total 23 Six primary USPC cell lines were assessed flow cytometry real‐time polymerase chain reaction (PCR) for...
Background Neutrophil gelatinase-associated lipocalin (NGAL) is emerging as a mediator of various biological and pathological states. However, the specific role this molecule remains unclear, it serves biomarker for many conditions. The high sensitivity NGAL coupled with relatively low specificity may hide important roles. Data point toward an acute compensatory, protective in response to adverse cellular stresses, including inflammatory oxidative stress. aim study was understand whether...
We performed serological and molecular pretransplant screening in solid organ transplant (SOT) donors recipients north central Italy a surveillance program for human herpesvirus 8 (HHV8) infection after transplant, aiming to establish an optimal management of HHV8 SOT recipients.For both recipients, 6 (4 indirect immunofluorescent assays [IFA] 2 enzyme-linked immunosorbent assays-both lytic latent antigen based) were used. A reference standard identify HHV8-positive patients was defined by...
High-grade serous ovarian carcinoma (HGSOC) is generally associated with a very dismal prognosis. Nevertheless, patients similar clinicopathological characteristics can have markedly different clinical outcomes. Our aim was the identification of novel molecular determinants influencing survival. Gene expression profiles extreme HGSOC survivors (training set) were obtained by microarray. Differentially expressed genes (DEGs) and enriched signalling pathways determined. A prognostic signature...
We evaluated the expression of human trophoblastic cell-surface marker (Trop-2) and potential hRS7 - a humanized monoclonal anti-Trop-2 antibody as therapeutic strategy against treatment-refractory uterine (UMMT) ovarian (OMMT) carcinosarcoma cell lines.Trop-2 was by immunohistochemistry (IHC) in paraffin-embedded tumor tissues, real-time polymerase-chain-reaction (RT-PCR) flow-cytometry lines. Sensitivity to antibody-dependent cellular cytotoxicity (ADCC) complement-dependent tested using...
Abstract Background Cervical cancer continues to be an important worldwide health problem for women. Up 35% of patients who are diagnosed with and appropriately treated cervical will recur treatment results poor recurrent disease. Given these sobering statistics, development novel therapies remains a high priority. We evaluated the expression Tissue Factor (TF) in potential hI-con1, antibody-like-molecule targeted against TF, as form immunotherapy multiple primary carcinoma cell lines...
Carcinosarcomas of the female genital tract are rare tumors with an aggressive clinical behavior. Trastuzumab, a humanized monoclonal antibody, acts by binding to HER2/neu extracellular domain and exhibits therapeutic efficacy in HER2/neu-overexpressing cancers. Two uterine carcinosarcomas (UMMT-ARK-1, UMMT-ARK-2) 2 ovarian (OMMT-ARK-1, OMMT-ARK-2) were established as primary tumor cell lines vitro evaluated for expression immunohistochemistry, fluorescent situ hybridization analysis,...
Abstract Background Development of innovative, effective therapies against recurrent/chemotherapy-resistant ovarian cancer remains a high priority. Using high-throughput technologies to analyze genetic fingerprints cancer, we have discovered extremely expression the genes encoding proteins claudin-3 and claudin-4. Methods Because -4 are epithelial receptors for Clostridium perfringens enterotoxin (CPE), sufficient mediate CPE binding, in this study evaluated vitro vivo bioactivity...
Human epididymis protein 4 (HE4), kallikrein 6 (KLK6), osteopontin (OPN) and soluble mesothelin-related peptide (SMRP) are new promising biomarkers that could integrate CA125 in epithelial ovarian cancer (EOC) diagnosis. The autoantibody response to tumor antigens is a potential tool for improving the diagnostic performances of biomarkers. aim this study was assess these form free markers immunocomplexed with immunoglobulin M (IgM). Moreover, we analyzed association between...
AIMTo evaluate the effect of a 12-mo supervised aerobic and resistance training, on renal function exercise capacity compared to usual care recommendations. METHODSNinety-nine kidney transplant recipients (KTRs) were assigned interventional (Group A; n = 52) cohort B; 47).Blood urine chemistry, capacity, muscular strength, anthropometric measures health-related quality life (HRQoL) assessed at baseline, after 6 12 mo.Group A underwent training three times per week for B received only general...