A5103119837- Urinary Bladder and Prostate Research
- Hormonal and reproductive studies
- Vitamin D Research Studies
- Estrogen and related hormone effects
- Adrenal Hormones and Disorders
- Sexual function and dysfunction studies
- Sexual Differentiation and Disorders
- Clusterin in disease pathology
- Chemokine receptors and signaling
- Biomarkers in Disease Mechanisms
- Coronary Interventions and Diagnostics
- Cytokine Signaling Pathways and Interactions
- Aldose Reductase and Taurine
- Acute Myocardial Infarction Research
- Heart Failure Treatment and Management
- Regulation of Appetite and Obesity
- Hemophilia Treatment and Research
- Ion channel regulation and function
- Fibroblast Growth Factor Research
- Ubiquitin and proteasome pathways
- Thyroid Cancer Diagnosis and Treatment
- Hypothalamic control of reproductive hormones
- Neuroendocrine regulation and behavior
- Cardiac Health and Mental Health
- Bone health and treatments
Foro Italico University of Rome
2010-2019
University of Florence
1993-2010
Kingston Health Sciences Centre
2003
Merck & Co., Inc., Rahway, NJ, USA (United States)
2003
Kitamurayama Hospital
2003
Maebashi Institute of Technology
2003
Abstract Benign prostatic hyperplasia (BPH), a highly prevalent condition, could involve an inflammatory component in disease pathogenesis. In this study, we show that human stromal prostate cells obtained from BPH tissue can actively contribute to the process by secreting proinflammatory cytokines as well chemokines able recruit lymphomonuclear and acting APCs. express all of TLRs their ligation leads secretion CXCL8/IL-8, CXCL10, IL-6. addition, costimulatory class I II MHC molecules,...
We report for the first time that penile smooth muscle cells (SMC) not only respond to, but also synthesize, endothelin-1 (ET-1), one of main regulators SMC activity. Immunohistochemical studies indicated that, beside endothelial (EC), human adult and fetal penis express ET-1 its converting enzyme, ECE-1. Accordingly, cultures stromal these genes. prepared characterized from fetuses. These specific markers such as α actin phosphodiesterase type 5A3 along with hallmarks androgen-dependent...
OBJECTIVE: Calcitriol analogues might represent an interesting new therapy for benign prostate hyperplasia (BPH). We here report the preclinical characterization of BXL-628, analogue selected ongoing double-blind, randomized, placebo-controlled phase II trial in BPH. DESIGN: Experiments with BXL-628 were carried out human BPH cells and ventral intact castrated rats. METHODS: cell rat growth evaluated along morphological biochemical hallmarks apoptosis. RESULTS: inhibited proliferation...
Abstract BACKGROUND BXL‐628 is a calcitriol analog shown to decrease prostate growth in preclinical and clinical studies. BPH symptoms are generated not only by overgrowth but also bladder overactivity, resulting from an increased RhoA/Rho‐kinase signaling. Because smooth muscle cells express VDR, we studied effects of on this pathway. METHODS RhoA Rho‐kinase gene expression functional activity were rat human real‐time RT‐PCR, immuno‐kinase assays, western blot analysis, confocal microscopy,...
Chemokines are a large family of cytokines involved in the pathogenesis inflammatory and autoimmune diseases. Among CXC chemokines, chemokine ligand 10 (CXCL10) has been identified to play an important role several endocrinological diseases, such as Hashimoto's thyroiditis, Graves' disease, type 1 diabetes mellitus. Although mechanisms leading glandular process may be at least part shared by different endocrine organs, CXCL10 adrenal insufficiency is unknown. The aim this study was evaluate...
We have recently found that analog V (BXL-353, a calcitriol analog) inhibits growth factor (GF)-stimulated human benign prostate hyperplasia (BPH) cell proliferation by disrupting signal transduction, reducing Bcl-2 expression, and inducing apoptosis. now report BXL-353 blocks in vitro vivo testosterone (T) activity. BPH cells responded to T dihydrotestosterone (DHT) with dose-dependent reduced Exposure of significantly antagonized both T- DHT-induced induced apoptosis, even the presence T....
In a previous report, we demonstrated that in FNC-B4 cells, derived and characterized from human fetal olfactory epithelium, both sex steroids odorants regulate GnRH secretion. We now report the presence biological activity of endothelin (ET)-1 this GnRH-secreting neuronal cell. By situ hybridization immunohistochemistry, found gene protein expression ET-1 its converting enzyme ECE-1 mucosa cells. The authentic conditioned media cells was further supported by combined RIAs high-performance...
[125I]Atrial natriuretic peptide (ANP) was used to identify ANP receptors on a clonal line of bovine bone endothelial (BBE) cells. Specific binding [125I]ANP saturable and high affinity. Computer analysis the equilibrium data indicated that Scatchard plots are best fit by straight (Kd = 69.3 +/- 20.9 pM; capacity 37.9 fmol/10(6) cells). The order potency for competing with human (hANP) > rat atriopeptin-1 (rAP-1) porcine brain (pBNP) C-type peptide. Affinity cross-linking studies presence...
INTRODUCTION AND OBJECTIVE: To improve our ability of monitoring prostate gland in live animals and to study the molecular steps carcinogenesis, we developed a transgenic mouse model overexpressing luciferase (luc) gene under control human super prostate-specific antigen (sPSA) promoter (Yeung, et al. mc 275: 40846, 2000).This is used development assess quantitatively effect androgen stimulating growth using non-invasive imaging technique.This will confirm specificity sPSA for transgene...
You have accessJournal of UrologyDiscussed Poster, Sunday, May 21, 2006, 9:00 am - 12:00 pm1 Apr 2006190: BXL-628, a Vitamin D Analog, Decreases RHOA/ROK Signalling in Rat and Human Bladder Annamaria Morelli, Sandra Filippi, Rosa Mancina, Linda Vignozzi, Gabriella B. Vannelli, Stefano Ambrosini, Clara Crescioli, Silvia Donati, Luciano Adorini, Mario Maggi MorelliAnnamaria Morelli More articles by this author , FilippiSandra Filippi MancinaRosa Mancina VignozziLinda Vignozzi VannelliGabriella...
You have accessJournal of UrologyDiscussed Poster, Tuesday, May 11, 2004, 8:00 am - 12:00 pm1 Apr 20041349: Inhibition Spontaneous and Androgen-Induced Prostate Growth by a Nonhypercalcemic Calcitriol Analogue Donata Villari, Clara Crescioli, Pietro Ferruzzi, Rosa Mancina, Gabrilella B. Vannelli, Saverio Bettuzzi, Vincenzo Li Marzi, Michelangelo Rizzo, Giulio Nicita, Mario Serio, Luciano Adorini, Maggi VillariDonata Villari More articles this author , CrescioliClara Crescioli FerruzziPietro...