A5005595984- Vitamin D Research Studies
- Inflammatory Myopathies and Dermatomyositis
- Muscle Physiology and Disorders
- Prostate Cancer Treatment and Research
- Estrogen and related hormone effects
- Vitamin C and Antioxidants Research
- Mitochondrial Function and Pathology
- Peripheral Artery Disease Management
- Immune Cell Function and Interaction
- Cancer Cells and Metastasis
- Bone health and treatments
- Cardiac Ischemia and Reperfusion
- Immune Response and Inflammation
- Nanoparticles: synthesis and applications
- Muscle and Compartmental Disorders
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- Adipose Tissue and Metabolism
- Psoriasis: Treatment and Pathogenesis
- Hormonal and reproductive studies
- Lipoproteins and Cardiovascular Health
- Biotin and Related Studies
- Urinary Bladder and Prostate Research
- T-cell and B-cell Immunology
- Virus-based gene therapy research
Inserm
2012-2025
Université de Strasbourg
2015-2025
Centre National de la Recherche Scientifique
2015-2025
Institut de génétique et de biologie moléculaire et cellulaire
2015-2025
Institut de Biologie Moléculaire et Cellulaire
2012-2022
Collège de France
2015
Laboratoire de Chémo-biologie synthétique et thérapeutique
2011
Biogen (Italy)
2007-2010
Brown University
2009
Hospital de Sant Pau
2009
<h3>Background & aims</h3> Inflammatory bowel disease (IBD) is characterised by chronic intestinal inflammation, resulting from dysregulation of the mucosal immune system and compromised epithelial barrier function. The bile salt, nuclear farnesoid X receptor (FXR), was recently implicated in antibacterial defence aim this study to investigate therapeutic potential FXR agonists treatment inflammation complementary vivo vitro models. <h3>Methods</h3> Colitis induced wild-type (WT)...
Prevalence of asthma is higher in women than men, but the mechanisms underlying this sex bias are unknown. Group 2 innate lymphoid cells (ILC2s) key regulators type inflammatory responses. Here, we show that ILC2 development greatly influenced by male hormones. Male mice have reduced numbers progenitors (ILC2Ps) and mature ILC2s peripheral tissues compared with females. In consequence, males exhibit susceptibility to allergic airway inflammation response environmental allergens less severe...
Abstract Benign prostatic hyperplasia (BPH), a highly prevalent condition, could involve an inflammatory component in disease pathogenesis. In this study, we show that human stromal prostate cells obtained from BPH tissue can actively contribute to the process by secreting proinflammatory cytokines as well chemokines able recruit lymphomonuclear and acting APCs. express all of TLRs their ligation leads secretion CXCL8/IL-8, CXCL10, IL-6. addition, costimulatory class I II MHC molecules,...
Nutritional vitamin D supplements are often used in general pediatrics. Here, the aim is to address supplementation and calcium nutritional intakes newborns, infants, children, adolescents prevent deficiency rickets populations.We formulated clinical questions relating following categories: Patient (or Population) whom recommendation will apply; Intervention being considered; Comparison (which may be "no action," placebo, or an alternative intervention); Outcomes affected by intervention...
Abstract BACKGROUND Benign prostatic hyperplasia (BPH) is characterized by an important inflammatory component. Stimulation of human prostate stromal cells from BPH tissues with proinflammatory cytokines leads to secretion IL‐8, a chemokine involved in pathogenesis. The vitamin D receptor (VDR) agonist elocalcitol can arrest growth patients, but its mechanism action this pathology still incompletely understood. METHODS IL‐8 levels were measured real‐time RT‐PCR and ELISA. NF‐κB translocation...
The transcriptional coregulators PGC-1α and PGC-1β modulate the expression of numerous partially overlapping genes involved in mitochondrial biogenesis energetic metabolism. physiological role is poorly understood skeletal muscle, a tissue high content to produce ATP levels required for sustained contractions. Here we determine muscle using mice, which selectively ablated myofibres at adulthood (PGC-1β((i)skm-/-) mice). We show that myofibre myosin heavy chain composition number, strength...
Prostate cancer (PCa) is a leading cause of cancer-related deaths. The slow evolution precancerous lesions to malignant tumors provides broad time frame for preventing PCa. To characterize prostatic intraepithelial neoplasia (PIN) progression, we conducted longitudinal studies on Pten(i)pe-/- mice that recapitulate prostate carcinogenesis in humans. We found early PINs are hypoxic and hypoxia-inducible factor 1 alpha (HIF1A) signaling activated luminal cells, thus enhancing progression....
The bioactive form of vitamin D [1,25(OH)2D3] regulates mineral and bone homeostasis exerts potent anti-inflammatory antiproliferative properties through binding to the receptor (VDR). 3D structures VDR ligand-binding domain with 1,25(OH)2D3 or gemini analogs unveiled molecular mechanism underlying ligand recognition. On basis structure-function correlations, we generated a point-mutated (VDRgem) that is unresponsive 1,25(OH)2D3, but activity which efficiently induced by ligands. Moreover,...
Abstract Synthetic glucocorticoids (GC), such as dexamethasone, are extensively used to treat chronic inflammation and autoimmune disorders. However, long-term treatments limited by various side effects, including muscle atrophy. GC activities mediated the glucocorticoid receptor (GR), that regulates target gene expression in tissues association with cell-specific co-regulators. Here we show GR lysine-specific demethylase 1 (LSD1) interact myofibers of male mice, LSD1 connects GR-bound...
Abstract Skeletal muscle is a dynamic tissue the size of which can be remodeled through concerted actions various cues. Here, we investigated skeletal transcriptional program and identified key tissue-specific regulatory genetic elements. Our results show that Myod1 bound to numerous enhancers in collaboration with glucocorticoid receptor (GR) control gene expression. Remarkably, activation controlled by these factors occurs direct contacts promoter region target genes, via CpG-bound...
Objectives: Diabetic patients respond poorly to revascularization for peripheral arterial disease (PAD) but the underlying mechanisms are not well understood. We aimed determine whether diabetes worsens ischemia-reperfusion (IR)-induced muscle dysfunction and involvement of endogenous protective kinases in this process. Materials Methods: Streptozotocin-induced diabetic non-diabetic rats were randomized control or IR injury (3 h aortic cross-clamping 2 reperfusion). Mitochondrial...
Castration-resistant prostate cancer (CRPC) is a common male malignancy that requires new therapeutic strategies due to acquired resistance its first-line treatment, docetaxel. The benefits of vitamin D on (PCa) progression have been previously reported. This study aimed investigate the effects chemoresistance in CRPC.
Abstract Prostate cancer is a heterogeneous disease with slow progression and highly variable clinical outcome. The tumor suppressor genes PTEN TP53 are frequently mutated in prostate predictive of early metastatic dissemination unfavorable patient outcomes. solid tumors to metastasis often associated increased cell plasticity, but the complex events underlying -loss-induced aggressiveness remain incompletely understood. Using genetically engineered mice, we show that Trp53 deficiency Pten...
A critical knowledge gap in prostate cancer research is understanding whether castration-tolerant progenitor-like cells that reside treatment-naive tumors play a direct role therapy resistance and tumor progression. Herein, we reveal the castration tolerance of LSCmed (Lin-, Sca-1+, CD49fmed) progenitor cells, mouse equivalent human prostatic Club arises not from intrinsic properties but significant transcriptional reprogramming. Utilizing single-cell RNA sequencing isolated...
Inflammatory myopathies (IM) are a group of severe autoimmune diseases, sharing some similarities, whose cause is unknown and treatment empirical.While C-protein-induced myositis (CIM), the most currently used mouse model IM, has removed roadblocks to understand improve it only been partially characterised its generation limited by poor reproducibility. This study aimed at optimising characterisation CIM. In silico analysis was run identify top three specific immunogenic regions C-protein....
1alpha,25(OH)(2)-16-ene-20-cyclopropyl-vitamin D(3) (13) is several fold more potent than the natural hormone 1alpha,25-dihydroxyvitamin (1) as an anti-inflammatory agent. Here, we have further analyzed properties of 13, confirming it most analogue tested within this family. We then determined structures all metabolites including 24-oxo metabolite 14, and carried out its synthesis. A comparison 13 with 14 showed a similar induction primary VDR target genes CYP24A1 CAMP comparable revealed by...
The bioactive vitamin D3, 1α,25(OH)2D3, plays a central role in calcium homeostasis by controlling the activity of D receptor (VDR) various tissues. Hypercalcemia secondary to high circulating levels D3 leads hypercalciuria, nephrocalcinosis and renal dysfunctions. Current therapeutic strategies aim at limiting intake, absorption resorption, or 1α,25(OH)2D3 synthesis, but are poorly efficient. In this study, we identify WBP4 as new VDR interactant, demonstrate that it controls subcellular...
Epidemiological data have linked vitamin D deficiency to the onset and severity of various cancers, including prostate cancer, although in vitro studies demonstrated anticancer activities for D, clinical trials provided conflicting results. To determine impact signaling on prostatic precancerous lesions, we treated genetically engineered Pten(i)pe-/- mice harboring intraepithelial neoplasia (PIN) with Gemini-72, a analog reported activities. We show that this induces apoptosis senescent...