A5081819845- Cardiac electrophysiology and arrhythmias
- Cardiomyopathy and Myosin Studies
- Genetic Neurodegenerative Diseases
- Neuroscience and Neuropharmacology Research
- Neuroendocrine regulation and behavior
- Cardiovascular Effects of Exercise
- Cardiovascular Function and Risk Factors
- Neuroscience of respiration and sleep
- Stress Responses and Cortisol
- Photoreceptor and optogenetics research
- Cardiac Imaging and Diagnostics
- Mitochondrial Function and Pathology
- ECG Monitoring and Analysis
- Pluripotent Stem Cells Research
- Memory and Neural Mechanisms
- Receptor Mechanisms and Signaling
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Photochromic and Fluorescence Chemistry
- Neuroscience and Neural Engineering
- Radiomics and Machine Learning in Medical Imaging
- Neuroinflammation and Neurodegeneration Mechanisms
- Evolutionary Psychology and Human Behavior
- Cardiac Fibrosis and Remodeling
- Neurological disorders and treatments
University of Milano-Bicocca
2021-2024
University of Pavia
2018-2021
Abstract Aims The heterozygous phospholamban (PLN) mutation R14del (PLN +/− ) is associated with a severe arrhythmogenic cardiomyopathy (ACM) developing in the adult. “Superinhibition” of SERCA2a by PLN widely assumed to underlie pathogenesis, but alternative mechanisms such abnormal energy metabolism have also been reported. This work aims (1) evaluate Ca 2+ dynamics and transgenic (TG) mouse model prior development; (2) test whether they are causally connected. Methods dynamics,...
Huntington disease (HD) is an inherited late-onset neurological disorder characterized by progressive neuronal loss and disruption of cortical basal ganglia circuits. Cell replacement using human embryonic stem cells may offer the opportunity to repair damaged circuits significantly ameliorate conditions. Here, we showed that in-vitro-differentiated striatal progenitors undergo maturation integrate into host upon intra-striatal transplantation in a rat model HD. By combining graft-specific...
Abstract Brain cholesterol is produced mainly by astrocytes and important for neuronal function. Its biosynthesis severely reduced in mouse models of Huntington’s disease. One possible mechanism a diminished nuclear translocation the transcription factor sterol regulatory element-binding protein 2 (SREBP2) and, consequently, activation SREBP2-controlled genes pathway. Here we evaluated efficacy gene therapy based on unilateral intra-striatal injection recombinant adeno-associated virus 2/5...
Non-genetic photostimulation is a novel and rapidly growing multidisciplinary field that aims to induce light-sensitivity in living systems by exploiting exogeneous phototransducers. Here, we propose an intramembrane photoswitch, based on azobenzene derivative (Ziapin2), for optical pacing of human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). The light-mediated stimulation process has been studied applying several techniques detect the effect cell properties. In...
Oxytocin is a neuropeptide that plays important peripheral and central neuromodulatory functions. Our data show that, following activation of oxytocin receptors (OtRs) with the selective agonist TGOT (Thr4,Gly7-oxytocin), significant increase in frequency amplitude spontaneous inhibitory postsynaptic currents (sIPSC) occurred hippocampal CA1 pyramidal neurons (PYR) mice. affected also sIPSC deactivation kinetics, suggesting involvement perisynaptic GABAA (GABAARs) as well. By contrast, did...
A variety of pathophysiological mechanisms are implicated in Huntington's disease (HD). Among them, reduced cholesterol biosynthesis has been detected the HD mouse brain from pre-symptomatic stages, leading to diminished synthesis, particularly striatum. In addition, systemic injection cholesterol-loaded brain-permeable nanoparticles ameliorates synaptic and cognitive function a transgenic model HD. To identify an appropriate treatment regimen gain mechanistic insights into beneficial...
ABSTRACT Aims The heterozygous phospholamban (PLN) mutation R14del (PLN +/- ) is associated with a severe arrhythmogenic cardiomyopathy (ACM) developing in the adult. “Superinhibition” of SERCA2a by PLN widely assumed to underlie pathogenesis, but alternative mechanisms such abnormal energy metabolism have also been reported. This work aims 1) evaluate Ca 2+ dynamics and transgenic (TG) mouse model prior development; 2) test whether they are causally connected. Methods Results dynamics,...
The perirhinal cortex (PRC) is a polymodal associative region of the temporal lobe that works as gateway between cortical areas and hippocampus. In recent years, an increasing interest arose in role played by PRC learning memory processes, such object recognition memory, contrast with certain forms hippocampus-dependent spatial episodic memory. integrative properties should provide all necessary resources to select enhance information be propagated from Among these properties, we explore...
Oxytocin (OT) is a neuropeptide that modulates social-related behavior and cognition in the central nervous system of mammals. In CA1 area hippocampus, indirect effects OT on pyramidal neurons their role information processing have been elucidated. However, limited data are available concerning direct modulation exerted by interneurons (INs) expressing oxytocin receptor (OTR). Here, we demonstrated TGOT (Thr4,Gly7-oxytocin), selective OTR agonist, affects not only membrane potential firing...
The stem cell technology and the induced pluripotent cells (iPSCs) production represent an excellent alternative tool to study cardiomyopathies, which overcome limitations associated with primary cardiomyocytes (CMs) access manipulation. CMs from human iPSCs (hiPSC–CMs) are genetically identical patient of origin, main electrophysiological mechanical features CMs. key issue be solved is achieve a degree structural functional maturity typical adult In this perspective, we will focus on...
There are numerous studies showing a reduction in cholesterol biosynthesis the brain of different HD animal models since pre-symptomatic stages, with striatum being more affected compared to other tissues (Valenza et al., 2005; 2007; 2010; Shankaran 2017). Abnormalities homeostasis also present patients: specific metabolite 24-hydroxy-cholesterol (24OHC) is progressively reduced plasma patients and pre-HD manifesting individuals who close onset disease (Leoni 2008; 2013). On hand, we...
Abstract A variety of pathophysiological mechanisms are implicated in Huntington’s disease (HD). Among them, reduced cholesterol biosynthesis has been detected the HD mouse brain from pre-symptomatic stages, leading to diminished synthesis, particularly striatum. In addition, systemic injection cholesterol-loaded brain-permeable nanoparticles ameliorates synaptic and cognitive function a transgenic model HD. To identify an appropriate treatment regimen gain mechanistic insights into...
Abstract Introduction Long QT Syndrome (LQTS) is an arrhythmogenic disorder caused either by genetic alteration or exogenous factors. Polycystic Ovary (PCOS) a disease associated with high levels of testosterone and low estrogen (E2). It has been suggested that hormones can influence the occurrence malignant cardiac events in LQTS type 2 (LQT2) women, carriers mutations on potassium channel (hERG). However, precise mechanisms governing this process still need to be clarified. Purpose We...
Abstract Brain cholesterol is produced mainly by astrocytes and important for neuronal function. Its biosynthesis severely reduced in mouse models of Huntington’s Disease (HD). One possible mechanism a diminished nuclear translocation the transcription factor sterol regulatory element binding protein 2 (SREBP2) and, consequently, activation SREBP-controlled genes pathway. Here we evaluated efficacy gene therapy based on unilateral intra-striatal injection recombinant adeno-associated virus...
Abstract Non-genetic photostimulation is a novel and rapidly growing multidisciplinary field of research that aims to induce light sensitivity in living systems by exploiting exogeneous phototransducers. Here we propose recently synthetized intramembrane photoswitch, based on an azobenzene derivative (Ziapin2), for optical pacing human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). The light-mediated stimulation process has been studied applying several characterization...