A5019227514- Coffee research and impacts
- Alzheimer's disease research and treatments
- Advanced Chemical Sensor Technologies
- Metabolomics and Mass Spectrometry Studies
- GABA and Rice Research
- Immune Response and Inflammation
- Medicinal Plants and Neuroprotection
- Transplantation: Methods and Outcomes
- Pneumonia and Respiratory Infections
- Food Chemistry and Fat Analysis
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Natural Antidiabetic Agents Studies
- Tea Polyphenols and Effects
- Immune Cell Function and Interaction
- Antimicrobial Peptides and Activities
- Respiratory viral infections research
- Cocoa and Sweet Potato Agronomy
- Isotope Analysis in Ecology
- Lipid metabolism and biosynthesis
- Bacterial Infections and Vaccines
- Medicinal Plants and Bioactive Compounds
- Drug Transport and Resistance Mechanisms
- Nanocluster Synthesis and Applications
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Atomic and Subatomic Physics Research
University of Milano-Bicocca
2013-2025
The structure–activity relationship was investigated in a series of synthetic TLR4 antagonists formed by glucosamine core linked to two phosphate esters and linear carbon chains. Molecular modeling showed that the compounds with 10, 12, 14 carbons chains are associated higher stabilization MD-2/TLR4 antagonist conformation than case C16 variant. Binding experiments human MD-2 C12 C14 variants have affinity C10, while variant did not interact protein. molecules, exception variant, inhibited...
Abstract Monosaccharide lipid A mimetics based on a glucosamine core linked to two fatty acid chains and bearing one or phosphate groups have been synthesized. Compounds 1 2 , each with group, were practically inactive in inhibiting LPS‐induced TLR4 signaling cytokine production HEK‐blue cells murine macrophages, but compound 3 groups, was found be active efficiently signal both cell types. The direct interaction between the MD‐2 coreceptor investigated by NMR spectroscopy molecular...
Recent epidemiological studies have demonstrated that the consumption of healthy foods are particularly rich in polyphenols might reduce incidence cancer and neurodegenerative diseases. In particular, chlorogenic acids (CGAs) occur ubiquitously food represent most abundant human diet. A number beneficial biological effects CGAs, such as anti-inflammatory activity, anti-carcinogenic protection against diseases, been reported. However, molecular mechanisms at base these activities not yet...
Eleven calix[4]arene ligands, bearing zwitterionic α‐amino acids or charged ammonium sulfonate/carboxylate groups, are synthesized and screened for the binding to envelopes of three bacterial strain representatives Gram‐positive, Gram‐negative, mycobacteria. The is followed by on‐cell Saturation Transfer Difference Nuclear Magnetic Resonance (NMR) experiments directly on alive cells. While anionic tetrasulfonatocalixarene does not bind any strains significantly cationic calixarenes strongly...
Abstract Human immunodeficiency virus type-1 (HIV-1)-associated neurocognitive disorder (HAND) remains an important neurological manifestation that adversely affects a patient’s quality of life. HIV-1 matrix protein p17 (p17) has been detected in autoptic brain tissue HAND individuals who presented early with severe AIDS encephalopathy. We hypothesised the ability to misfold may result generation toxic assemblies and be relevant for pathogenesis. A multidisciplinary integrated approach...
The metabolomic analysis of exhaled breath condensate (EBC) may provide insights on both the pathology pulmonary disorders and response to therapy. This pilot study describes ability nuclear magnetic resonance (NMR)-based metabolomics discriminate α1-antitrypsin deficient (AATD)-patients, who were diagnosed with moderate severe emphysema, from healthy individuals. Comparative samples these two homogeneous cohorts individuals resulted in generation NMR profiles that different a qualitative...
This report describes the application of NMR spectroscopy to profiling metabolites in bronchoalveolar lavage fluid (BALf) lung transplant recipients without bronchiolitis obliterans syndrome (BOS) (stable, S, n = 10), and with BOS at different degrees severity (BOS 0p, 10; I, 10). Through fine-tuning a number parameters concerning both sample preparation/processing variations spectra acquisition modes, an efficient reproducible protocol was designed for screening pulmonary that should...
The anti-Alzheimer disease (AD) activity reported for an aqueous cinnamon bark extract prompted us to investigate and compare the anti-amyloidogenic properties of extracts obtained from both bud, latter being a very little explored matrix. We prepared with different procedures (alcoholic, hydroalcoholic, or extractions). An efficient protocol rapid analysis NMR spectra bud was set up, enabling automatic identification quantification metabolites. Moreover, we exploited preparative...
Abstract Lipopolysaccharide (LPS), the main cell‐surface molecular constituent of Gram‐negative bacteria, is synthesized in inner membrane (IM) and transported to outer (OM) by Lpt (lipopolysaccharide transport) machinery. Neosynthesized LPS first flipped MsbA across IM, then OM seven proteins located IM (LptBCFG), periplasm (LptA), (LptDE). A functional essential bacterial viability requires correct placement leaflet. Therefore, biogenesis represents an ideal target for development novel...
The cationic glycolipid IAXO-102, a potent TLR4 antagonist targeting both MD-2 and CD14 co-receptors, has been used as scaffold to design new potential modulators fluorescent labels for the receptor complex (membrane TLR4.MD-2 dimer CD14). primary amino group of not involved in direct interaction with receptors, exploited covalently attach fluorescein (molecules 1 2) or link two molecules IAXO-102 through diamine diammonium spacers, obtaining 'dimeric' 3 4. structure-based rational compounds...
The anti-inflammatory activity of coffee extracts is widely recognized and supported by experimental evidence, in both vitro vivo settings, mainly murine models. Here, we investigated the immunomodulatory properties from green (GCE) medium-roasted (RCE) Coffea canephora beans human macrophages. biological effect GCE RCE was characterized LPS-stimulated THP-1-derived macrophages (TDM) as a model inflammation. Results showed decreased amounts TNF-α, IL-6 IL-1β strong dose-dependent inhibition...
Spinocerebellar ataxia type 3 (SCA3) is a neurodegenerative disorder characterized by and other neurological manifestations, with poor prognosis lack of effective therapies. The amyloid aggregation the ataxin-3 protein hallmark SCA3 one main biochemical events prompting its onset, making it prominent target for development preventive therapeutic interventions. Here, we tested efficacy an aqueous Lavado cocoa extract polyphenolic components against neurotoxicity. combination assays atomic...
<b>Purpose:</b> Tools that will help unraveling the complexity of Bronchiolitis Obliterans Syndrome (BOS) and identifying useful predictive markers are urgently needed. Given its ability capturing disease-relevant metabolic profile changes, Metabolomics may occupy an important role in this area. <b>Methods:</b> BALf was obtained fromlung transplant patients, before after BOS development,and from subjects affected by other respiratory diseases. Samples were submitted to NMR analyses,through...
There is a pressing need to identify biomarkers that are useful monitor effects of expensive treatments such as i.v. alpha-1-antitrypsin (AAT) augmentation. We applied Nuclear Magnetic Resonance (NMR) spectroscopy <b>single</b>, blinded samples exhaled breath condensate (EBC) from 11 patients with moderate severe emphysema and PiZZ- AAT deficiency spouse healthy controls. The spectral metabolomics profiles showed the presence 20 25 metabolites propionic acid, acetate, butyrate, benzoate,...
In Antarctica, ice-free areas can be found along the coast, on mountain peaks, and in McMurdo Dry Valleys, where microorganisms well-adapted to harsh conditions survive reproduce. Metabolic analyses shed light survival mechanisms of Antarctic soil communities from both coastal sites, under different plant coverage stages, inner sites slow-growing or dormant microorganisms, low water availability, salt accumulation, a limited number primary producers make metabolomic profiling difficult....