A5101660839- Chronic Obstructive Pulmonary Disease (COPD) Research
- Amphibian and Reptile Biology
- Animal Behavior and Reproduction
- Connective tissue disorders research
- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- Advanced Chemical Sensor Technologies
- Protease and Inhibitor Mechanisms
- Neurobiology and Insect Physiology Research
- Mass Spectrometry Techniques and Applications
- Insect and Pesticide Research
- Bone and Dental Protein Studies
- Transplantation: Methods and Outcomes
- Microfluidic and Capillary Electrophoresis Applications
- Analytical Chemistry and Chromatography
- thermodynamics and calorimetric analyses
- Cancer therapeutics and mechanisms
- Microbial metabolism and enzyme function
- Muscle metabolism and nutrition
- Asthma and respiratory diseases
- Insect and Arachnid Ecology and Behavior
- Cystic Fibrosis Research Advances
- Peptidase Inhibition and Analysis
- Bacterial Genetics and Biotechnology
- Respiratory viral infections research
Vita-Salute San Raffaele University
2025
University of Toronto
2025
University of Pavia
2015-2024
Abstract Lysyl hydroxylases catalyze hydroxylation of collagen lysines, and sustain essential roles in extracellular matrix (ECM) maturation remodeling. Malfunctions these enzymes cause severe connective tissue disorders. Human lysyl hydroxylase 3 (LH3/PLOD3) bears multiple enzymatic activities, as it catalyzes lysine also their subsequent glycosylation. Our understanding LH3 functions is currently hampered by lack molecular structure information. Here, we present high resolution crystal...
Three pools of exhaled breath condensate (EBC) from non-smokers plus healthy smokers (NS + HS, n = 45); chronic obstructive pulmonary disease (COPD) without emphysema (COPD, 15) and subjects with associated α1-antitrypsin deficiency (AATD, 23) were used for an exploratory proteomic study aimed at generating fingerprints these groups that can be in future pathophysiological perhaps even clinical research. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was the platform applied this...
Abstract Background The magnitude and factors associated with levodopa equivalent daily dose (LEDD) reduction in deep brain stimulation (DBS) of the globus pallidus interna (GPi) for Parkinson's Disease (PD) remain unclear. Objective To investigate LEDD GPi DBS its correlation anatomical/clinical characteristics. Methods A consecutive cohort 74 patients who had undergone was analyzed. Regression probabilistic efficacy mapping were performed to evaluate predicting reduction. Results 32.4%...
Hard ticks are hematophagous arthropods that act as vectors of numerous pathogenic microorganisms high relevance in human and veterinary medicine. Ixodes ricinus is one the most important tick species Europe, due to its role vector bacteria such Borrelia burgdorferi Anaplasma phagocytophilum, viruses borne encephalitis virus protozoans Babesia spp. In addition these pathogens, I. harbors a symbiotic bacterium, Midichloria mitochondrii. This dominant associated ricinus, but biological not yet...
Desmosine and isodesmosine are products of elastin breakdown which candidate biomarkers to measure lung destruction in COPD. Data exist on the burden desmosines urine plasma COPD but long-term changes have never been investigated. We determined desmosine levels over 14 months patients with type ZZ alpha-1-antitryspsin deficiency-related Urines for determination were collected from 11 ex-smokers moderate/severe emphysema at monthly intervals months. Spirometry gas transfer assessed baseline...
This report describes the application of NMR spectroscopy to profiling metabolites in bronchoalveolar lavage fluid (BALf) lung transplant recipients without bronchiolitis obliterans syndrome (BOS) (stable, S, n = 10), and with BOS at different degrees severity (BOS 0p, 10; I, 10). Through fine-tuning a number parameters concerning both sample preparation/processing variations spectra acquisition modes, an efficient reproducible protocol was designed for screening pulmonary that should...
Abstract Colour polymorphism occurs when two or more genetically-based colour morphs permanently coexist within an interbreeding population. Colouration is usually associated to other life-history traits (ecological, physiological, behavioural, reproductive …) of the bearer, thus being phenotypic marker such set genetic features. This visual badge may be used inform conspecifics and drive those decision making processes which contribute maintaining under sexual selection context. The...
Synopsis Chemical communication in terrestrial vertebrates is often built on complex blends, where semiochemical and structural compounds may form an integrated functional unit. In lizards, many species have specialized epidermal glands whose secretions are waxy, homogeneous blends of lipids proteins, both active communication. The intimate co-occurrence such allows us to hypothesize that they should undergo a certain degree covariation, considering their role the support-to-lipid function...
Nasu-Hakola disease (NHD) is a recessively inherited rare disorder characterized by combination of neuropsychiatric and bone symptoms which, while being unique to this disease, do not provide rationale for the unambiguous identification patients. These individuals, in fact, are likely go unrecognized either because they considered be affected other kinds dementia or fibrous dysplasia bone. Given that NHD has much common with Alzheimer's neurodegenerative disorders, it cannot expected achieve...
Femoral glands of male lizards produce waxy secretions that are involved in inter- and intraspecific chemical communication. The main components these proteins lipids, the latter having been extensively studied already associated to quality. On opposite, composition role nearly unknown, only available information coming from few studies on iguanids. These got conclusion might have a communicative function, notably they could signal individual identity. A generalization findings requires...
Quorum sensing (QS) is a bacterial intercellular communication process which controls the production of major virulence factors, such as proteases, siderophores and toxins, well biofilm formation. Since inhibition this pathway reduces virulence, QS considered valuable candidate drug target, particularly for treatment opportunistic infections, those caused by Burkholderia cenocepacia in cystic fibrosis patients. Diketopiperazine inhibitors acyl homoserine lactone synthase CepI have been...
The mosquito proboscis is an efficient microelectromechanical system, which allows the insect to feed on vertebrate blood quickly and painlessly. Its efficiency further enhanced by saliva, although through unclear mechanisms. Here, we describe initial trigger of unprecedented feedback signaling pathway in Aedes mosquitoes affecting feeding behavior. We identified LIPS proteins saliva that promote skin. show a new all-helical protein fold constituted two domains. N-terminal domain interacts...
It is not known whether postsurgery systemic inflammation and plasma amino acid abnormalities are still present during rehabilitation of individuals after elective hip arthroplasty (EHA). Sixty subjects (36 females; age 66.58 ± 8.37 years) were randomized to receive 14-day oral EAAs (8 g/day) or a placebo (maltodextrin). At admission discharge from the center, serum C-reactive protein (CRP) venous concentrations determined. Post-EHA function was evaluated by Harris score (HHS) test. Ten...
A novel coumarin-based molecule, designed as a fluorescent surrogate of thiacetazone-derived antitubercular agent, was quickly and easily synthesized from readily available starting materials. This small coined Coum-TAC, exhibited combination appropriate physicochemical biological properties, including resistance toward hydrolysis excellent efficiency similar to that well-known thiacetazone derivatives, well efficient covalent labeling HadA, relevant therapeutic target combat Mycobacterium...
Burkholderia cenocepacia is a major concern for people suffering from cystic fibrosis as it contributes to serious respiratory tract infections. The lack of drugs effective against this opportunistic pathogen, along with the high level resistance multiple antibiotics, render treatment these infections particularly difficult. Here new compound, belonging 2,1,3-benzothiadiazol-5-yl family (10126109), bactericidal effect and minimal inhibitory concentration (MIC) 8 μg/ml B. cenocepacia,...
Human SERPINA1 gene is located on chromosome 14q31-32.3 and organized into three (IA, IB, IC) non-coding four (II, III, IV, V) coding exons. This produces α1-antitrypsin (A1AT), a prototypical member of the serpin superfamily proteins. We demonstrate that human peripheral blood leukocytes express not only product corresponding to transcript for full-length A1AT protein but also two short transcripts (ST1C4 ST1C5) A1AT. In silico sequence analysis revealed last exon contains an Open Reading...