A5068008634- Immune Response and Inflammation
- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- Pneumonia and Respiratory Infections
- Antimicrobial Peptides and Activities
- Surfactants and Colloidal Systems
- Nonlinear Optical Materials Research
- Porphyrin and Phthalocyanine Chemistry
- Pediatric health and respiratory diseases
- Advanced Photocatalysis Techniques
- Advanced Chemical Sensor Technologies
- Covalent Organic Framework Applications
- Organic and Molecular Conductors Research
- Molecular Sensors and Ion Detection
- NF-κB Signaling Pathways
- Inflammatory mediators and NSAID effects
- Pharmaceutical and Antibiotic Environmental Impacts
- Environmental Toxicology and Ecotoxicology
- Microfluidic and Capillary Electrophoresis Applications
- Isotope Analysis in Ecology
- Electronic and Structural Properties of Oxides
- Neurogenetic and Muscular Disorders Research
- Edible Oils Quality and Analysis
- Liver Disease Diagnosis and Treatment
Centre National de la Recherche Scientifique
2020-2024
Protéomique, Réponse Inflammatoire et Spectrométrie de Masse
2022-2024
Institut des Sciences Analytiques
2023-2024
Université Claude Bernard Lyon 1
2023-2024
Laboratoire COBRA
2019-2023
Université de Rouen Normandie
2020-2023
Normandie Université
2020-2023
Institut National des Sciences Appliquées Rouen Normandie
2020-2023
University of Milano-Bicocca
2010-2018
Génétique Moléculaire Génomique Microbiologie
2018
Toll-like receptor 4 (TLR4), together with MD-2, binds bacterial endotoxins (E) high affinity, triggering formation of the activated homodimer (E.MD-2.TLR4)2. Activated TLR4 induces intracellular signaling leading to activation transcription factors that result in cytokine and chemokine production initiation inflammatory immune responses. also responds endogenous ligands called danger associated molecular patterns (DAMPs). Increased sensitivity infection a variety pathologies have been...
A strategy for creating a general-purposes surface functionalization platform is reported, based on direct attachment of phosphate groups onto hydroxylated surfaces and subsequent formation terpyridine-based monolayer. Such suitable the construction, technologically relevant oxide surfaces, single- multilayer structures interest in technological applications. In particular, paper describes successful 4-(2,2′:6′,2′′-terpyridine-4-yl)benzenephosphonic acid (1, PPTP) SiO2 previously...
A thiophene-based donor-acceptor phenothiazine dye has been functionalized with a peripheral glucose unit (PTZ-GLU) to bust its affinity water and enhance dye-sensitized photogeneration of hydrogen. Compared the corresponding alkyl derivative (PTZ-ALK), as well common hydrophilic triethylene glycol substitution (PTZ-TEG), sugar shows lower contact angle; PTZ-GLU performed twice more efficient than PTZ-TEG in hydrogen terms evolved gas turnover number.
Soils with a history of 2,4-dichlorophenoxyacetic acid (2,4-D) treatment at field application rates and control soils no prior exposure to 2,4-D were amended in the laboratory. Before during these treatments, populations 2,4-D-degrading bacteria monitored by most-probable-number (MPN) enumeration hybridization analyses, using probes for tfd genes plasmid pJP4, which encode enzymes degradation. Data obtained alternate methods compared. Several months after most recent (approximately 1 ppm),...
The structure–activity relationship was investigated in a series of synthetic TLR4 antagonists formed by glucosamine core linked to two phosphate esters and linear carbon chains. Molecular modeling showed that the compounds with 10, 12, 14 carbons chains are associated higher stabilization MD-2/TLR4 antagonist conformation than case C16 variant. Binding experiments human MD-2 C12 C14 variants have affinity C10, while variant did not interact protein. molecules, exception variant, inhibited...
Two simple approaches to build up micropatterned functionalized polymer films are reported here. Both of them based on the formation porous consisting two-dimensionally ordered void structures, produced by evaporation a solution in presence humidity. In first approach, we utilize an amino-terminated linear polystyrene fabricate honeycomb structured which cavities enriched with amino groups that preserve their chemical reactivity. This allows, principle, obtain different surface functionality...
Abstract Monosaccharide lipid A mimetics based on a glucosamine core linked to two fatty acid chains and bearing one or phosphate groups have been synthesized. Compounds 1 2 , each with group, were practically inactive in inhibiting LPS‐induced TLR4 signaling cytokine production HEK‐blue cells murine macrophages, but compound 3 groups, was found be active efficiently signal both cell types. The direct interaction between the MD‐2 coreceptor investigated by NMR spectroscopy molecular...
An increasing number of pathologies have been linked to Toll-like receptor 4 (TLR4) activation and signaling, therefore new hit lead compounds targeting this process are urgently needed. We report on the synthesis biological properties glycolipids based glucose trehalose scaffolds which potently inhibit TLR4 signaling in vitro vivo. Structure-activity relationship studies these indicate that presence fatty ester chains molecule is a primary prerequisite for activity point facial...
The hepatitis C virus (HCV) nonstructural (NS) protein 5A is a multifunctional that plays central role in viral replication and assembly. Antiviral agents directly targeting NS5A are currently clinical development. Although the elucidation of mechanism action (MOA) inhibitors has been focus intensive research, detailed understanding how these exert their antiviral effect still lacking. In this study, we observed downregulation hyperphosphorylation associated with actions belonging to...
Abstract Lipopolysaccharide (LPS), the main cell‐surface molecular constituent of Gram‐negative bacteria, is synthesized in inner membrane (IM) and transported to outer (OM) by Lpt (lipopolysaccharide transport) machinery. Neosynthesized LPS first flipped MsbA across IM, then OM seven proteins located IM (LptBCFG), periplasm (LptA), (LptDE). A functional essential bacterial viability requires correct placement leaflet. Therefore, biogenesis represents an ideal target for development novel...
The sincerest form of flattery! A novel, symmetric lipid mimetic (D1) formed by two glucose units linked through a 6-6' succinic diamide linker is active in modulating the activity human TLR4. D1 inhibits endotoxin-stimulated TLR4 activation inhibiting interaction endotoxin with both receptors CD14 and MD-2 (associated to TLR4). also has weak agonist that makes it promising lead compound for development as vaccine adjuvant.
An extensive spectroscopic analysis is presented of an elongated polycondensed dye with a donor-acceptor substitution. The charge-transfer (CT) state, polarized along the long molecular axis, close in energy to local excitation (LE) system, roughly short which makes this system particularly suitable investigate subtle LE/CT interplay. essential-state model that quantitatively reproduces absorption and fluorescence spectra, as well emission anisotropy spectra collected solvents different...
The cationic glycolipid IAXO-102, a potent TLR4 antagonist targeting both MD-2 and CD14 co-receptors, has been used as scaffold to design new potential modulators fluorescent labels for the receptor complex (membrane TLR4.MD-2 dimer CD14). primary amino group of not involved in direct interaction with receptors, exploited covalently attach fluorescein (molecules 1 2) or link two molecules IAXO-102 through diamine diammonium spacers, obtaining 'dimeric' 3 4. structure-based rational compounds...
DIA (Data-Independent Acquisition) is a powerful technique in Liquid Chromatography coupled with high-resolution tandem Mass Spectrometry (LC-MS/MS) initially developed for proteomics studies and recently emerging metabolomics lipidomics. It provides comprehensive unbiased coverage of molecules improved reproducibility quantitative accuracy compared to Data-Dependent Acquisition (DDA). Combined the Zeno trap Electron-Activated Dissociation (EAD), enhances data quality structural elucidation...
The preparation of materials featuring enhanced second harmonic generation (SHG) values by self-assembly molecules characterized high second-order non-linear optic (NLO) activity is nowadays an important and challenging field research. In order to show SHG the material must have acentric structure with dipoles molecular components oriented in same direction this synthetically fairly difficult achieve. This study describes synthesis push–pull stilbazolium salt 1 its assembly multilayered thin...
Rationale Self‐assembling processes of surfactants in the gas phase constitute a developing research field interest since they allow information to be gained on peculiar structural organization these aggregates, their ability incorporate from small molecules up proteins and possible use as carriers drugs or cleaning agents exotic reaction media. Methods The mass spectra charged aggregates chiral surfactant (1 R ,2 S )‐dodecyl(2‐hydroxy‐1‐methyl‐2‐phenylethyl)dimethylammonium bromide (DMEB)...