A5015825651- Neuroinflammation and Neurodegeneration Mechanisms
- Amyotrophic Lateral Sclerosis Research
- Nerve injury and regeneration
- Spinal Cord Injury Research
- Neurogenesis and neuroplasticity mechanisms
- Neurogenetic and Muscular Disorders Research
- Nanoparticle-Based Drug Delivery
- Alzheimer's disease research and treatments
- Long-Term Effects of COVID-19
- PARP inhibition in cancer therapy
- Immune cells in cancer
- Erythropoietin and Anemia Treatment
- Graphene and Nanomaterials Applications
- Neuroscience and Neuropharmacology Research
- Insect and Pesticide Research
- RNA Interference and Gene Delivery
- Toxic Organic Pollutants Impact
- Mesenchymal stem cell research
- Insect and Arachnid Ecology and Behavior
- Pesticide Exposure and Toxicity
- Vagus Nerve Stimulation Research
- Biopolymer Synthesis and Applications
- Advanced Drug Delivery Systems
- Pesticide Residue Analysis and Safety
- Pregnancy-related medical research
Mario Negri Institute for Pharmacological Research
2014-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2014-2022
Mylan (Switzerland)
2017
Erythropoietin (EPO), a member of the type 1 cytokine superfamily, plays critical hormonal role regulating erythrocyte production as well paracrine/autocrine in which locally produced EPO protects wide variety tissues from diverse injuries. Significantly, these functions are mediated by distinct receptors: hematopoiesis via receptor homodimer and tissue protection heterocomplex composed CD131, β common receptor. In present work, we have delimited tissue-protective domains within to short...
Much evidence shows that acute and chronic inflammation in spinal cord injury (SCI), characterized by immune cell infiltration release of inflammatory mediators, is implicated development the secondary phase occurs after trauma worsening damage. Activation microglia/macrophages associated response appears to be a self-propelling mechanism leads progressive neurodegeneration persisting pain state. Recent advances polymer science have provided huge amount innovations leading increased interest...
Astrogliosis has a very dynamic response during the progression of spinal cord injury, with beneficial or detrimental effects on recovery. It is therefore important to develop strategies target activated astrocytes and their harmful molecular mechanisms so as promote protective environment counteract secondary injury. The challenge formulate an effective therapy maximum effects, but reduced side effects. In this study, functionalized nanogel-based nanovector was selectively internalized in...
Chronic treatment with asialo erythropoietin (ASIALO-EPO) or carbamylated (CEPO) improved motor behavior and reduced motoneuron loss astrocyte microglia activation in the cervical spinal cord of wobbler mice, an animal model amyotrophic lateral sclerosis, but had no effect on hematocrit values. ASIALO-EPO CEPO, like parent compound EPO, protected primary cultures from kainate-induced death vitro. Both EPO receptor common CD131 β chain were expressed cultured motoneurons anterior horn mice...
Sustained inflammatory reactions are common pathological events associated with neuron loss in neurodegenerative diseases. Reported evidence suggests that Toll-like receptor 4 (TLR4) is a key player of neuroinflammation several However, the mechanisms by which TLR4 mediates neurotoxic signals remain poorly understood. We investigated role vitro and vivosettings motor degeneration. Using primary cultures from mouse spinal cords, we characterized both proinflammatory effects activation...
The major histocompatibility complex I (MHCI) is a key molecule for the interaction of mononucleated cells with CD8+T lymphocytes. We previously showed that MHCI upregulated in spinal cord microglia and motor axons transgenic SOD1G93A mice.To assess role disease, we examined mice crossbred β2 microglobulin-deficient mice, which express little if any on cell surface are defective CD8+ T cells.The lack sciatic nerve affects axon stability, anticipating muscle atrophy disease onset. In...
Abstract Current treatments for modulating the glial‐mediated inflammatory response after spinal cord injury (SCI) have limited ability to improve recovery. This is quite likely due lack of a selective therapeutic approach acting on microgliosis and astrocytosis, glia components most involved trauma, while maximizing efficacy minimizing side effects. A new nanogel that can selectively release active compounds in microglial cells astrocytes developed characterized. The degree selectivity...
Abstract A new paradigm for the aetiopathology of phenylketonuria suggests presence amyloid-like assemblies in brains transgenic mouse models and patients with phenylketonuria, possibly shedding light on selective cognitive deficit associated this disease. Paralleling amyloidogenic route that identifies different stages peptide aggregation, corresponding to levels toxicity, we experimentally address first time, physico-chemical properties phenylalanine aggregates via Small Angle, Wide Angle...
Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease that affects the motor neuromuscular system leading to complete paralysis and premature death. The multifactorial nature of ALS involves both cell-autonomous non-cell-autonomous processes contributes lack effective therapies, usually targeted single pathogenic mechanism. RNS60, an experimental drug containing oxygenated nanobubbles generated by modified Taylor-Couette-Poiseuille flow with elevated oxygen pressure,...
Brain ischemia is a common acute injury resulting from impaired blood flow to the brain. Translation of effective drug candidates experimental models patients has systematically failed. The use human induced pluripotent stem cells (iPSC) offers new opportunities gain translational insights into diseases including brain ischemia. We used 3D self-assembling iPSC-derived model (human cortical organoids, hCO) characterize effects caused by oxygen-glucose deprivation (OGD). hCO exposed 2 h or 8...
<i>Objectives:</i> Chemokines are implicated in many diseases of the central nervous system (CNS). Although their primary role is to induce inflammation through recruitment leukocytes by chemotactic activity, they may also have direct effects on neuronal cells. We evaluated expression CXCR1 and CXCR2 investigated effect activation agonist MIP-2 (CXCL2) cultured motor neurons. To specifically assess neurotoxicity induced MIP-2, we used CXCR1/2 inhibitor reparixin studied chemokine...
Phagocytosis is a key function of myeloid cells and highly involved in brain ischemic injury. It has been scarcely studied vivo, thus preventing deep knowledge the processes occurring environment. Structured illumination microscopy (SIM) superresolution technique which helps study phagocytosis, process involving recruitment vesicles sized below resolution limits standard confocal microscopy. Mice underwent permanent occlusion middle cerebral artery were sacrificed at 48 h or 7 days after...
CXCL13 is a B and T lymphocyte chemokine that mediates neuroinflammation through its receptor CXCR5. This highly expressed by motoneurons (MNs) in Amyotrophic Lateral Sclerosis (ALS) SOD1G93A (mSOD1) mice during the disease, particularly fast-progressing mice. Accordingly, this study, we investigated role of ALS.
The central nervous system (CNS) is extremely vulnerable to the toxic effects of environmental pollutants during development. Polybrominated diphenyl ethers (PBDEs) are persistent contaminants, increasingly present in environment and human tissues. Recent investigations identified a correlation between maternal exposure PBDEs impairment fetal neurobehavioral development, suggesting that these contaminants pose potential risk for children. We investigated on decabrominated ether (decaBDE,...
One of the most important drawbacks nanomedicine is related to unwanted rapid diffusion drugs loaded within nanocarriers towards external biological environment, according high clearance body fluids.