A5074581566- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Hydrogels: synthesis, properties, applications
- Nanoparticle-Based Drug Delivery
- Immune Response and Inflammation
- 3D Printing in Biomedical Research
- Nerve injury and regeneration
- Nitric Oxide and Endothelin Effects
- Advanced Drug Delivery Systems
- Graphene and Nanomaterials Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Spinal Cord Injury Research
- Bone Tissue Engineering Materials
- Electrospun Nanofibers in Biomedical Applications
- Supramolecular Self-Assembly in Materials
- Immune cells in cancer
- biodegradable polymer synthesis and properties
- Advanced Glycation End Products research
- Alzheimer's disease research and treatments
- S100 Proteins and Annexins
- Erythrocyte Function and Pathophysiology
- Phagocytosis and Immune Regulation
- RNA Interference and Gene Delivery
- Advanced Sensor and Energy Harvesting Materials
- Neurogenesis and neuroplasticity mechanisms
- Mesenchymal stem cell research
Politecnico di Milano
2016-2025
Università della Svizzera italiana
2023-2024
University of Applied Sciences and Arts of Southern Switzerland
2024
National Research Council
1964-2021
Istituto di Fotonica e Nanotecnologie
2021
Italian Institute of Technology
2021
Consorzio Milano Ricerche
2021
Mylan (Switzerland)
2017-2020
GTx (United States)
2020
Curculio Institute
2017-2019
After phagocytosis of yeast opsonized with IgG, neutrophil leukocytes (polymorphonuclear [PMN]) expressed high levels neutrophil-activating peptide 1/interleukin 8 (NAP-1/IL-8) mRNA, which peaked after 3-5 h and were still elevated 18 h. A similar but quantitatively less prominent effect was obtained lipopolysaccharide (LPS). phagocytosis, not exposure to LPS, the PMN progressively released considerable amounts NAP-1/IL-8 into culture medium (18.6-50 ng/ml in h). The biologically active, as...
Abstract Excessive and unwarranted administration of antibiotics has invigorated the evolution multidrug‐resistant microbes. There is, therefore, an urgent need for advanced active compounds. Ionic liquids with short‐lived ion‐pair structures are highly tunable have diverse applications. Apart from their unique physicochemical features, newly discovered biological activities ionic fascinated biochemists, microbiologists, medical scientists. In particular, antimicrobial properties opened new...
Bone healing is an impressive ability of the human body, but critical-sized bone defects require external intervention. Bioceramic scaffolds with excellent biocompatibility and bioactivity have been developed to treat non-healing because their unique features for repair. Meanwhile, mechanical properties material continue be disadvantageous. This review focuses on (i) essential factors in affecting improving bioceramic-based scaffolds' properties, including porosity, pore size, methods,...
The extracellular matrix can be replicated by 3D scaffolds, providing a favorable environment for cell growth, proliferation, and differentiation. Despite their biocompatibility, biodegradability, bioactivity, the poor mechanical strength of scaffolds limits use heavy loads. This creates bottleneck in supply with enhanced all previously mentioned characteristics. Conjugated polymers have emerged as promising option scaffold construction due to electrical conductivity, adjustable surface...
The deposition of β-amyloid in the brain is key pathogenetic event Alzheimer's disease. Among various mechanisms proposed to explain neurotoxicity deposits, a new one, recently identified our and other laboratories, suggests that indirectly neurotoxic by activating microglia produce toxic inflammatory mediators such as cytokines, nitric oxide, oxygen free radicals. Three findings presented here support this mechanism, showing peptides (25Goodwin J.L. Uemura E. Cunnick J.E. Brain Res. 1995;...
Helicobacter pylori infection induces the appearance of inflammatory infiltrates, consisting mainly neutrophils and monocytes, in human gastric mucosa. A bacterial protein with neutrophil activating activity (HP-NAP) has been previously identified, but its role immune response is still largely unknown. Here, we show that vaccination mice HP-NAP protection against H. challenge, majority infected patients produce antibodies specific for HP-NAP, suggesting an important this factor immunity. We...
In this study, we analyzed the expression of genes encoding for components phagocyte superoxide anion-generating system in human phagocytes treated with interferon-gamma (IFN-gamma) or lipopolysaccharide (LPS). Human neutrophils express high levels 47-kDa cytosolic factor (p47-phox), which are down-regulated after treatment IFN-gamma, but not LPS. On contrary, steady-state heavy chain subunit cytochrome b558 (gp91-phox) were increased by IFN-gamma and LPS monocyte-derived macrophages a time-...
To address the question whether leukocyte integrins are able to generate signals activating neutrophil functions, we investigated capability of mAbs against common beta chain (CD18), or distinct alpha chains CR3, LFA-1, gp150/95, activate respiratory burst. These investigations were performed with bound protein A immobilized tissue culture polystyrene. Neutrophils plated in wells coated anti-CD18 IB4 and 60.3 released H2O2; H2O2 release did not occur when neutrophils an irrelevant,...
Sulfatides have been established recently as ligands for L-selectin, and we investigated whether they trigger transmembrane signals through ligation of L-selectin.We found that sulfatides the increase cytosolic free calcium in neutrophils this effect was strictly dependent on sulfation galactose ring, non-sulfated galactocerebrosides were not stimulatory.Chymotrypsin phorbol12-myristate 13-acetate treatment caused shedding but class I major histocompatibility complex antigens or /32...
Much evidence shows that acute and chronic inflammation in spinal cord injury (SCI), characterized by immune cell infiltration release of inflammatory mediators, is implicated development the secondary phase occurs after trauma worsening damage. Activation microglia/macrophages associated response appears to be a self-propelling mechanism leads progressive neurodegeneration persisting pain state. Recent advances polymer science have provided huge amount innovations leading increased interest...