A5046675430- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- SARS-CoV-2 and COVID-19 Research
- Biomarkers in Disease Mechanisms
- Immune cells in cancer
- Advanced biosensing and bioanalysis techniques
- Respiratory viral infections research
- Pancreatic and Hepatic Oncology Research
- Mesenchymal stem cell research
- Pharmaceutical and Antibiotic Environmental Impacts
- HIV Research and Treatment
- Viral gastroenteritis research and epidemiology
- Polysaccharides and Plant Cell Walls
- Extracellular vesicles in disease
- Macrophage Migration Inhibitory Factor
- Nanocluster Synthesis and Applications
- Advanced Glycation End Products research
- COVID-19 diagnosis using AI
- Glycosylation and Glycoproteins Research
- Galectins and Cancer Biology
- Bone Metabolism and Diseases
- COVID-19 Clinical Research Studies
- Glutathione Transferases and Polymorphisms
- Phagocytosis and Immune Regulation
- Biofuel production and bioconversion
Istituti di Ricovero e Cura a Carattere Scientifico
2010-2025
Mario Negri Institute for Pharmacological Research
2009-2025
Norges Røde Kors
2022
Dulbecco Telethon Institute
2018
Abstract Background Cocaine use seems to be increasing in some urban areas worldwide, but it is not straightforward determine the real extent of this phenomenon. Trends drug abuse are currently estimated indirectly, mainly by large-scale social, medical, and crime statistics that may biased or too generic. We thus tested a more direct approach based on 'field' evidence cocaine general population. Methods its main urinary metabolite (benzoylecgonine, BE) were measured mass spectrometry water...
Abstract Tumor-associated macrophages (TAMs) are key orchestrators of the tumor microenvironment directly affecting neoplastic cell growth, neoangiogenesis, and extracellular matrix remodeling. In turn, milieu strongly influences maturation TAMs shapes several their features. To address early macrophage (Mϕ) differentiation phase in a malignant context, we mimicked by vitro coculturing human blood monocytes with conditioned media from different cancer lines. Only 2 out 16 lines induced Mϕ...
The role of dendritic cells (DC) that accumulate in the renal parenchyma non-immune-mediated proteinuric nephropathies is not well understood. Under certain circumstances, DC capture immunologically ignored antigens, including self-antigens, and present them within MHC class I, initiating an autoimmune response. We studied whether could generate antigenic peptides from self-protein albumin. Exposure rat proximal tubular to autologous albumin resulted its proteolytic cleavage form N-terminal...
Selenoprotein N (SELENON) is an endoplasmic reticulum (ER) protein whose loss of function leads to human SELENON-related myopathies. SelenoN knockout (KO) mouse limb muscles, however, are protected from the disease, and display no major alterations in muscle histology or contractile properties. Interestingly, we find that highly active diaphragm shows impaired force production, line with phenotype. In addition, after repeated stimulation a protocol which induces fatigue, also hind muscles...
The cancer secretome is a rich repository in which to mine useful information for both biology and clinical oncology. To help understand the mechanisms underlying progression of pancreatic cancer, we characterized secretomes four human ductal adenocarcinoma (PDAC) cell lines versus normal counterpart. this end, used proteomic workflow based on high-confidence protein identification by mass spectrometry, semiquantitation label-free approach, network enrichment analysis system tool. Functional...
Abstract Human immunodeficiency virus type-1 (HIV-1)-associated neurocognitive disorder (HAND) remains an important neurological manifestation that adversely affects a patient’s quality of life. HIV-1 matrix protein p17 (p17) has been detected in autoptic brain tissue HAND individuals who presented early with severe AIDS encephalopathy. We hypothesised the ability to misfold may result generation toxic assemblies and be relevant for pathogenesis. A multidisciplinary integrated approach...
Wastewater-based environmental surveillance enables the monitoring of SARS-CoV-2 dynamics within populations, offering critical epidemiological insights. Numerous workflows for tracking have been developed globally, underscoring need interlaboratory comparisons to ensure data consistency and comparability. An inter-calibration test was conducted among laboratories network in wastewater samples across Lombardy region (Italy). The aimed evaluate reliability identify potential sources...
K-Ras mutations are a hallmark of human pancreatic adenocarcinoma (PDAC) and epithelial-mesenchymal-transition (EMT) is driver progression. Oncogenic causes the constitutive activation NF-kB switch-on an inflammatory program, which further fuels STAT3 activation. In this study we investigated how pathways triggered by oncogenic regulated in cancer cells with distict epithelial or mesenchymal phenotype. Our results demonstrate that features, driven inflammation under control IL-1, while...
The emergence of new SARS-CoV-2 variants poses challenges to global surveillance efforts, necessitating swift actions in their detection, evaluation, and management. Among the most recent variants, Omicron BA.2.86 its sub-lineages have gained attention due potential immune evasion properties. This study describes development a digital PCR assay for rapid detection descendant lineages, wastewater samples. By using this assay, we analyzed samples collected Italy from September 2023 January...
(1) Background: The surveillance of SARS-CoV-2 RNA in urban wastewaters allows one to monitor the presence virus a population, including asymptomatic and symptomatic individuals, capturing real circulation this pathogen. aim study was evaluate performance different pre-analytical analytical methods for identifying untreated municipal samples by conducting an inter-laboratory proficiency test. (2) Methods: three concentration, namely, (A) Dextran PEG-6000 two-phase separation, (B) PEG-8000...
Abstract The human immune deficiency virus type 1 (HIV-1) matrix protein p17 (p17), although devoid of a signal sequence, is released by infected cells and detected in blood different organs tissues even HIV-1-infected patients undergoing successful combined antiretroviral therapy (cART). Extracellularly, deregulates the function involved AIDS pathogenesis. mechanism secretion, particularly during HIV-1 latency, still remains to be elucidated. A recent study showed that can produce Gag...
The molecular characterization of bioactive food components is necessary for understanding the mechanisms their beneficial or detrimental effects on human health. This study focused γ-conglutin, a well-known lupin seed N-glycoprotein with health-promoting properties and controversial allergenic potential. Given importance N-glycosylation functional structural characteristics proteins, we studied purified protein by mass spectrometry-based glycoproteomic approach able to identify structure,...
Wastewater-based epidemiology (WBE) was conducted to track Enteroviruses (EVs) circulation in the Milan metropolitan area (Northern Italy) during Covid-19 pandemic (March 2020-December 2022). 202 composite 24-hour wastewater samples (WWSs) were collected weekly from March 24, 2020, December 29, 2022 at inlet of two treatment plants (WWTP) (1.5 million inhabitants). EV-RNA quantified and molecular characterization non-polio EVs (NPEV) performed by Sanger sequence analysis. Data WWS matched...
The fate and effects of 42 pharmaceuticals was studied in Lake Como (Italy), wastewater treatment plants delivering water to the lake, two rivers potable obtained from lake water. is one deepest largest lakes Northern Italy, serving important ecosystem services (i.e., drinking water, recreational, industrial, irrigation uses), some which are currently at risk giving current scarcity climate change scenarios. highest concentrations measured were those diclofenac, followed by carbamazepine,...
KRAS mutations characterize pancreatic cell transformation from the earliest stages of carcinogenesis, and are present in >95% ductal adenocarcinoma (PDAC) cases. In search novel biomarkers for early diagnosis PDAC, we identified proteins secreted by normal human line (HPDE) recently transformed inducing overexpression KRASG12V oncogene. We report a proteomic signature KRAS-induced proteins, which was confirmed surgical tumor samples resected PDAC patients. The putative diagnostic...
The aromatic amine 4-aminobiphenyl (4-ABP) is an environmental and occupational contaminant known to be a major etiological agent of human bladder cancer. 4-ABP metabolites are able form DNA adducts that may induce mutations initiate carcinogenesis. Cells exposed develop resistance the carcinogen. aim present study was detect identify proteins whose expression altered in carcinoma RT112 sub-lines selected for acquired 4-ABP, order disentangle mechanisms.Differential proteome analysis cell...
Wastewater-based epidemiology (WBE) is a recognized, dynamic approach to monitoring the transmission of pathogens in communities through urban wastewater. This study aimed detect and quantify influenza A B viruses Italian wastewater during 2022–2023 season (October 2022 April 2023). total 298 samples were collected from 67 treatment plants (WTPs) across country. These analyzed for (IAV, IBV) using primers originally developed by Centers Disease Control Prevention (CDC) real-time PCR adapted...
Abstract Wastewater-based viral surveillance is a promising approach to monitor the circulation of SARS-CoV-2 in general population. The aim this study was develop an analytical method detect RNA urban wastewater, be implemented framework network Lombardy region (Northern Italy). This area first hotspot COVID-19 Europe. Composite 24h samples were collected weekly eight cities from end-March mid-June 2020 (first peak epidemic). developed and optimized, involved virus concentration, using PEG...