A5025131036- Acute Ischemic Stroke Management
- Cerebrovascular and Carotid Artery Diseases
- Angiogenesis and VEGF in Cancer
- Venous Thromboembolism Diagnosis and Management
- Protease and Inhibitor Mechanisms
- Neurological Disorders and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurological Disease Mechanisms and Treatments
- Stroke Rehabilitation and Recovery
- Atherosclerosis and Cardiovascular Diseases
- Genetic Associations and Epidemiology
- Long-Term Effects of COVID-19
- Cell Adhesion Molecules Research
- Cerebrovascular and genetic disorders
- Epigenetics and DNA Methylation
- Suicide and Self-Harm Studies
- MicroRNA in disease regulation
- Cerebrospinal fluid and hydrocephalus
- Intracerebral and Subarachnoid Hemorrhage Research
- Alzheimer's disease research and treatments
- Cardiovascular Health and Disease Prevention
- Atrial Fibrillation Management and Outcomes
- Cancer-related molecular mechanisms research
- Antiplatelet Therapy and Cardiovascular Diseases
- Lipoproteins and Cardiovascular Health
Manchester Metropolitan University
2016-2025
University Hospital Mútua de Terrassa
2016-2025
Mútua Terrassa
2016-2025
Population Health Research Institute
2024
McMaster University
2024
Universitat Autònoma de Barcelona
2006-2023
Clinical Trial Investigators
2022-2023
Barcelonaβeta Brain Research Center
2023
Brain (Germany)
2023
Hospital Terrassa
2015-2023
Stroke is one of the most common causes mortality and morbidity in Western world. It results from occlusion a cerebral artery followed by severe disturbances blood supply through microvessels to brain tissue. Despite an extensive literature its pathophysiology poorly understood, this has severely impeded logical development therapy. Brains were obtained 10 patients aged 46 85 years with survival times 5 92 days after their stroke. Infarcted areas representative control tissues contralateral...
Cryptogenic strokes constitute 20 to 30% of ischemic strokes, and most cryptogenic are considered be embolic undetermined source. An earlier randomized trial showed that rivaroxaban is no more effective than aspirin in preventing recurrent stroke after a presumed from an Whether dabigatran would this type was unclear.
A complementary DNA that encodes a bovine brain, calmodulin-sensitive (type I) adenylylcyclase has been inserted into the baculovirus genome under control of strong polyhedron promoter. Expression recombinant in Sf9 cells using increases activity cell membranes to 10-20 nmol.min-1.mg-1 (approximately 0.1% membrane protein). The catalytic can be stimulated by Gs alpha, calmodulin, or forskolin, and it inhibited adenosine analogs G protein beta gamma subunit. specific purified approximates 5...
Cognitive manifestations associated with the severity of a novel coronavirus (COVID-19) infection are unknown. An early detection neuropsychological could modify risk subsequent irreversible impairment and further neurocognitive decline.
<h3>Importance</h3> Neurofibrillary tangles composed of aggregated tau protein are one the neuropathological hallmarks Alzheimer disease (AD) and correlate with clinical severity. Monoclonal antibodies targeting may have potential to ameliorate AD progression by slowing or stopping spread and/or accumulation pathological tau. <h3>Objective</h3> To evaluate safety efficacy monoclonal anti-tau antibody semorinemab in prodromal mild AD. <h3>Design, Setting, Participants</h3> This phase 2...
Accumulation of tau pathology in Alzheimer disease (AD) correlates with cognitive decline. Anti-tau immunotherapies were proposed as potential interventions AD. While antibodies targeting N-terminal failed to demonstrate clinical efficacy prodromal-to-mild AD, their utility at other stages was not evaluated prior studies. Lauriet is a phase 2 study an anti-tau monoclonal antibody, semorinemab, patients mild-to-moderate
The emerging evidence of the role glymphatic system (GS) in Alzheimer's disease (AD) provides new opportunities for intervention from earliest stages disease. aim study is to evaluate efficacy GS AD identify biomarkers. We performed a two-stage proteomic health using intravenous gadolinium-based contrast agent (GBCA) with serial T1 3T magnetic resonance imaging (MRI) individuals amnestic mild cognitive impairment (aMCI). In Stage 1 (evaluated Cohort aMCI participants (n = 11)), we correlated...
A cDNA that encodes type VI11 adenylyl cyclase has been isolated from two rat brain libraries.The open reading frame a 1248-amino acid protein predicted to have sets of six transmembrane spans and putative nucleotide binding domains as is characteristic other mammalian cyclases.T w o messages are detected in with estimated sizes 5.5 4.4 kilobases.In situ hybridization indicates the most abundantly expressed granule cells dentate gyrus, pyramidal hippocampal fields CA1-CA3, entorhinal cortex,...
Biochemical, immunological, and molecular cloning studies have suggested the existence of multiple forms adenylyl cyclase (EC 4.6.1.1). An cDNA clone (type II) was isolated from a rat brain library found to encode protein 1090 amino acids that homologous but distinct previously described Ca2+/calmodulin-stimulated bovine brain. Expression type II in an insect cell line resulted increased level activity insensitive Ca2+/calmodulin. Addition activated Gs alpha II-containing membranes enzyme...
Occlusion in cerebral vessels results ischemic stroke and is followed by proliferation of microvessels, ie, angiogenesis. The process particularly marked the border zone infarct, known as penumbra. This increase vascularization likely to be caused action angiogenic factors, such TGF-beta 1, which a powerful regulator angiogenesis.In this study we examined 10 brain samples from patients who suffered for expression mRNA encoding 1.The penumbra contained highest levels 1 mRNA, whereas normal...
Background and Purpose —Both vascular endothelial growth factor (VEGF) transforming factor-β1 (TGF-β1) are expressed in higher than normal concentrations the penumbra of patients after ischemic stroke. Because both cytokines central to processes angiogenesis, tissue inflammation, fibrosis, we performed serial measurements these with cerebral infarction determined their relationship stroke etiology volume. Methods —We serially (at days 0, 1, 3, 7, 14) measured serum levels VEGF active TGF-β1...
Abstract Continuous subcutaneous apomorphine infusion (CSAI) is, at present, an alternative option for advanced Parkinson's disease (PD) with motor fluctuations. We studied the evolution of patients PD and severe fluctuations long‐term treated CSAI. reviewed data from 82 (mean age, 67 ± 11.07; duration, 14.39 5.7 years) referred to 35 tertiary hospitals in Spain. These were (for least 3 months) CSAI tolerated procedure without serious side effects. compared baseline these (before CSAI) those...
Only very little is know about the neurovascular niche after cardioembolic stroke. Three processes implicated in neurorepair: angiogenesis, neurogenesis and synaptic plasticity, would be naturally produced adult brains, but also could stimulated through endogen neurorepair phenomena. Angiogenesis stimulation generates new vessels with aim to increase collateral circulation. Neurogenesis controlled by intrinsic genetic mechanisms growth factors ambiental are important. The leading process of...
Abstract A new murine monoclonal antibody (MAb), E‐9, has been raised using tissue‐cultured human umbilical vein endothelial cells. The antigen recognized by this MAb is a peptide of 170 kDa under non‐reducing conditions and 96 reducing conditions. E‐9 showed marked heterogeneity in its distribution various tissues. it was present vascular cells all tumours, foetal organs regenerating inflamed It stained few normal However, with the exception tonsils, staining tended to be weak limited blood...
Abstract Alzheimer’s disease (AD) increases dramatically in patients with ischaemic stroke. Monomeric C-reactive protein (mCRP) appears the ECM of tissue after stroke, associating microvasculature, neurons and AD-plaques, Aβ, also, being able to dissociate native-CRP into inflammatory, mCRP vivo . Here, injected hippocampal region mice was retained within retrosplenial tract dorsal 3rd ventrical surrounding major vessels. Mice developed behavioural/cognitive deficits 1 month, concomitant...
Prehospital transfer protocols are based on rapid access to reperfusion therapies for patients with ischemic stroke. The effect of different among receiving a final diagnosis intracerebral hemorrhage (ICH) is unknown.
Growth factors control two important processes in infarcted tissue, ie, angiogenesis and gliosis. We recently reported that transforming growth factor-beta1 (TGF-beta1) might be involved after ischemic stroke humans; here we present data of an extensive study on platelet-derived factor (PDGF) its receptors.We studied brain samples from patients who suffered for the expression mRNA encoding PDGF-A, PDGF-B, PDGF receptors (PDGF-R). Proteins were examined by Western blotting...
The conservation of amino acid sequence among types I-IV adenylylcyclase has made it possible to apply the polymerase chain reaction examine extent molecular diversity within this family enzymes. cDNA templates from rat heart, liver, kidney, guinea pig brain and testes, mouse skeletal muscle were amplified with primers specific sequences. Evidence was obtained for a total eight distinct gene products divisible into five subfamilies. Five correspond regions cloned forms adenylylcyclase, while...
We have isolated a 5199-nucleotide cDNA from mouse library containing an open reading frame encoding the 1099-amino acid type VII adenylyl cyclase protein. The protein is most closely related in primary structure to unpublished human clone (GenBank accession no. D25538) and II cyclase. Northern blot analysis demonstrates that mRNA abundant heart, spleen, lung. cAMP content rises rapidly HEK 293 cells overexpressing following treatment with phorbol ester, peaking by 4 min, while expressing...