A5013261663- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Viral Infections and Immunology Research
- Virus-based gene therapy research
- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- Congenital Anomalies and Fetal Surgery
- Cytomegalovirus and herpesvirus research
- Hepatitis Viruses Studies and Epidemiology
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- Parvovirus B19 Infection Studies
- Herpesvirus Infections and Treatments
- Vaccine Coverage and Hesitancy
- Energy Harvesting in Wireless Networks
- COVID-19 epidemiological studies
- Mycobacterium research and diagnosis
- Advanced Thermodynamic Systems and Engines
- Congenital Diaphragmatic Hernia Studies
- Hereditary Neurological Disorders
- Thermal Regulation in Medicine
- Polyomavirus and related diseases
- Neurological diseases and metabolism
- Botulinum Toxin and Related Neurological Disorders
University of Palermo
2016-2025
Azienda Ospedaliera Universitaria Policlinico "Paolo Giaccone" di Palermo
2017
University of Bari Aldo Moro
2017
Hungarian Academy of Sciences
2017
Chinese Academy of Medical Sciences & Peking Union Medical College
2017
University of Parma
2017
PromoFirenze
2014
Hospital Sant Joan de Déu Barcelona
2012
Istituto Superiore di Sanità
2011
Istituti di Ricovero e Cura a Carattere Scientifico
2004
Abstract Since 2001, human Metapneumovirus has been a significant cause of respiratory disease worldwide, and no vaccine or preventive treatment is currently available. The ELISA-based live virus microneutralization assay method to detect neutralizing antibodies against target pathogen. aim this study was demonstrate the suitability approach quantifying A1 B1 subtypes in serum samples. To standardize validate assay, we carried out analytical procedures according International Council...
ABSTRACT A rotavirus sample collection from 19 consecutive years was used to investigate the heterogeneity and dynamics of evolution G1 strains in a geographically defined population. Phylogenetic analysis VP7 gene sequences G1P[8] human showed circulation heterogeneous population comprising three lineages seven sublineages. Increases rotaviruses were apparently associated with introduction novel that exhibited multiple amino acid changes antigenic regions involved neutralization compared...
Surveillance of noroviruses in Italy identified the novel GII.17 human norovirus strain, Kawasaki 2014, February 2015. This strain emerged as a major cause gastroenteritis Asia during 2014/15, replacing pandemic GII.4 Sydney 2012, but being reported only sporadically elsewhere. is undergoing fast diversification and continuous monitoring important to understand evolution implement future strategies on vaccines.
Abstract We sought datasets with granular age distributions of rotavirus-positive disease presentations among children <5 years age, before the introduction rotavirus vaccines. identified 117 and fit parametric to each country dataset mortality stratum. calculated median cumulative proportion gastroenteritis events expected occur at ages between birth 5.0 years. The hospital admissions was 38 weeks (interquartile range [IQR], 25–58 weeks) in countries very high child 65 (IQR, 40–107 low...
Abstract Infection by an animal-like strain of rotavirus (PA260/97) was diagnosed in a child with gastroenteritis Palermo, Italy, 1997. Sequence analysis VP7, VP4, VP6, and NSP4 genes showed resemblance to G3P[3] canine identified Italy 1996. Dogs are potential source human viral pathogens.
The full-length genome sequence of a feline G3P[9] rotavirus (RV) strain, BA222, identified from the intestinal content an adult cat, was determined. Strain BA222 possessed G3-P[9]-I2-R2-C2-M2-A3-N1-T3-E2-H3 genomic constellation, differing substantially other RVs. Phylogenetic analyses each segment revealed common origins with selected animal and zoonotic human RVs, notably rare multi-reassortant RVs (Ita/PAI58/96 Ita/PAH136/96). Altogether, findings suggest that are genetically diverse may...
Using full-length genome sequence analysis, we investigated 2 rare G3P[9] human rotavirus strains isolated from children with diarrhea. The genomes were recognized as assortments of genes closely related to rotaviruses originating cats, ruminants, and humans. Results suggest multiple transmissions animal rotaviruses.
During 2012, a novel pandemic GII.4 norovirus variant, Sydney emerged worldwide. A signature of the variant was GII.Pe ORF1, in association with Apeldoorn 2008-like ORF2-ORF3 genes. We report detection recombinant 2012 strains, possessing ORF1 gene former New Orleans 2009.
COVID-19 is a current global threat, and the characterization of antibody response vitally important to update vaccine development strategies. In this study we assessed SARS-CoV-2 concentrations in positive patients (N = 272) subjects vaccinated with BNT162b2 m-RNA 1256). For each participant, socio-demographic data, vaccination records, serological analyses, infection status were collected. IgG antibodies against S1/S2 antigens detected. Almost all (99.8%) showed seropositivity...
Wastewater-based epidemiology is a well-established tool for detecting and monitoring the spread of enteric pathogens use illegal drugs in communities real time. Since only few studies Italy have investigated correlation between SARS-CoV-2 wastewater prevalence COVID-19 cases from clinical testing, we conducted one-year surveillance study Sicily to correlate load RNA reported cumulative 14 cities October 2021 September 2022. Furthermore, role variants subvariants increase number infections....
Group A rotaviruses (RVAs) are a leading cause of viral gastroenteritis in children, with G2P[4] RVA being one the most common human strains worldwide. The complete genome sequences nine strains, selected from 26-year archival collection (1985-2011) established Palermo, Italy, were determined. strain associated peak activity 1996 resembled reassortant identified Kenya 1982 and differed completely genomic make up more recent that circulated during 2004-2011. Conversely, 2004-2011 RVAs...
The study investigated the genetic diversity of human astroviruses (HAstVs) detected in children hospitalized with gastroenteritis Italy 2008-2009. A total 1321 faecal samples were collected Parma (northern Italy), Bari (southern and Palermo (Sicily) screened for presence HAstVs. RT-PCR amplification a portion at 5'-end ORF2 allowed detection HAstVs 3·95% patients. Four different genotypes (HAstV-1, HAstV-2, HAstV-4, HAstV-5) found to be circulating during period, HAstV-1 being predominant...
Abstract The surveillance network RotaNet‐Italia was established in 2007 order to investigate the diversity of co‐circulating rotavirus strains Italy, and provide a baseline for future assessment possible effects vaccine implementation selecting novel versus common strains. A total 2,645 from pediatric patients with acute diarrhea were collected over three consecutive seasons September 2006 through August 2009, partially characterized by standardized multiplex RT‐PCR. Most (89.1%) belonged...
Sicily was the first Italian region to introduce rotavirus (RV) vaccination with monovalent G1P[8] vaccine Rotarix® in May 2012. In this study, seasonal distribution and molecular characterization of RV strains detected over 19 years were compared understand effect on evolutionary dynamics human RVs. A total 7846 stool samples collected from children < 5 age, hospitalized acute gastroenteritis, tested for detection genotyping. Since 2013, coverage has progressively increased, while...
The emergence of new SARS-CoV-2 variants poses challenges to global surveillance efforts, necessitating swift actions in their detection, evaluation, and management. Among the most recent variants, Omicron BA.2.86 its sub-lineages have gained attention due potential immune evasion properties. This study describes development a digital PCR assay for rapid detection descendant lineages, wastewater samples. By using this assay, we analyzed samples collected Italy from September 2023 January...
Abstract Noroviruses were detected in 48.4% of 192 children (<3 years age) hospitalized for gastroenteritis Palermo, Italy, during 2004; predominant genotypes GGIIb/Hilversum and GGII.4 Hunter. Of with viral enteritis, 19.6% had a mixed norovirus-rotavirus infection. The severity infection was lower norovirus than rotavirus but increased co-infection.