A5108033253- Advanced Proteomics Techniques and Applications
- Biomarkers in Disease Mechanisms
- Advanced Glycation End Products research
- Protease and Inhibitor Mechanisms
- Cardiac and Coronary Surgery Techniques
- Blood Coagulation and Thrombosis Mechanisms
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Antioxidant Activity and Oxidative Stress
- Metabolomics and Mass Spectrometry Studies
- Neurological Disorders and Treatments
- Lipoproteins and Cardiovascular Health
- Cardiac Valve Diseases and Treatments
- Cardiovascular Disease and Adiposity
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Extracellular vesicles in disease
- Respiratory Support and Mechanisms
- Peptidase Inhibition and Analysis
- Platelet Disorders and Treatments
- Cancer, Lipids, and Metabolism
- Coronary Interventions and Diagnostics
- Adipokines, Inflammation, and Metabolic Diseases
- MicroRNA in disease regulation
- Healthcare Systems and Public Health
- Cell Adhesion Molecules Research
- Neuroinflammation and Neurodegeneration Mechanisms
University of Milano-Bicocca
2023-2025
Centro Cardiologico Monzino
2014-2023
Istituti di Ricovero e Cura a Carattere Scientifico
2010-2021
Mylan (Switzerland)
2017
University of Milan
2005-2015
Universidade Federal do Paraná
2009
Azienda Ospedaliera Sant'Andrea
2009
University of Washington
2009
Zero to Three
2009
Institute of Agricultural Biology and Biotechnology
2008
miR-210 is a key player of cell response to hypoxia, modulating survival, VEGF-driven endothelial migration, and the ability cells form capillary-like structures. A crucial step in understanding microRNA (miRNA) function identification their targets. However, only few targets have been identified date. Here, we describe an integrated strategy for large-scale new by combining transcriptomics proteomics with bioinformatic approaches. To experimentally validate candidate targets, RNA-induced...
Microparticles (MP) are small membrane vesicles that released from cells upon activation or during apoptosis. Cellular MP in body fluids constitute a heterogeneous population, differing cellular origin, numbers, size, antigenic composition and functional properties. support coagulation by exposure of tissue factor (TF), the initiator vivo. Moreover, may transfer bioactive molecules to other cells, thereby stimulating them produce cytokines, cell-adhesion molecules, growth factors TF,...
Epidemiological studies strongly suggest a link between stress, depression, and cardiovascular diseases (CVDs); the mechanistic correlation, however, is poorly understood. A single-nucleotide polymorphism in BDNF gene (BDNFVal66Met), associated with depression anxiety, has been proposed as genetic risk factor for CVD. Using knock-in mouse carrying BDNFVal66Met human polymorphism, which phenocopies psychiatric-related symptoms found humans, we investigated impact of this SNP on...
In the present paper, extracellular antioxidant activity of N-acetyl-cysteine (NAC) is explained by considering its ability to regenerate free form albumin Cys34 breaking disulfide bond cysteinylated (HSA-Cys). NAC's capability (HSA-SH) was studied MS intact protein analysis in human plasma and a concentration range NAC easily achievable after oral i.v. administration (5-50 µg/mL). dose-dependently broke HSA-Cys dimer NAC-Cys thus regenerating Cys34, whose reduced state maintained for at...
Cellular sex has rarely been considered as a biological variable in preclinical research, even when the pathogenesis of diseases with predictable differences is studied. In this perspective, proteomics, and "omics" approaches general, can provide powerful tools to obtain comprehensive cellular maps, thus favoring discovery still unknown sex-biased physio-pathological mechanisms. We performed proteomic Gene Ontology (GO) analyses secretome from human serum-deprived male female endothelial...
ABSTRACT Colorectal cancer is the third most common worldwide; its higher incidence in Western world, compared to rural areas of Africa and Asia, led classification among so‐called Westernized diseases. The wild strawberry Fragaria vesca L. endowed with several bioactive components, such as polyphenols, vitamins, terpenes, organic acids that can contribute prevention. In this study, we chemically characterized a extract through high‐resolution mass spectrometry evaluated antioxidant...
Development of heart failure depends on systemic and molecular abnormalities among which are the activation neurohormonal systems increase matrix metalloproteinases (MMPs). This study assessed relationship between catecholamines active MMPs in vivo patients with severe congestive (CHF) vitro human cardiac fibroblasts.Forty CHF due to dilated cardiomyopathy, either idiopathic (n=20) or secondary ischaemic disease (n=20), were compared 20 healthy subjects. Plasma MMP-2 MMP-9 activity, but not...
Spontaneously hypertensive stroke-prone rats (SHRSP) develop hypertension and systemic inflammation, with subsequent brain renal disorders early death. We tested the hypothesis that valsartan, an angiotensin II type 1 (AT1) receptor antagonist, exerts protective effects in SHRSP through its anti-inflammatory properties, even absence of a blood pressure-lowering effect. fed high-salt diet were treated vehicle or valsartan (1–10 mg/kg/day). The vehicle-treated developed hypertension,...
Gestational diabetes (GDM) is the most common complication of pregnancy and it associated with maternal fetal short- long-term consequences. GDM modifies placental structure function, but many underlying mechanisms are still unclear. The aim this study to develop compare two different methods, based respectively on gel-based gel-free proteomics, in order investigate proteome absence or presence identify, through a comparative approach, possible changes protein expression due condition....
Abstract This study shows that DKK-1, a member of the Dickkopf family and regulator Wnt pathways, represents novel target statins which, through inhibition HMG-CoA reductase non-steroidal isoprenoid intermediates, exert extra-beneficial effect in preventing atherosclerosis beyond their on lipid profile. We found atorvastatin downregulates DKK-1 protein (−88.3 ± 4.1%) mRNA expression (−90 4.2%) Cdc42, Rho Rac geranylgeranylated proteins. Further, combined approach based integration label-free...
Human serum albumin (HSA) is the most abundant circulating protein in body and presents an extensive range of biological functions. As such, it prone to undergo post-translational modifications (PTMs). The non-enzymatic early glycation HSA, one several PTMs undergone by arises from addition reducing sugars amine group residues, thus modifying structure HSA. These changes may affect HSA functions impairing its activity, finally leading cell damage. aim this study was quantitate glycated-HSA...