A5012710359- Nitric Oxide and Endothelin Effects
- Inflammatory mediators and NSAID effects
- Antiplatelet Therapy and Cardiovascular Diseases
- Antioxidant Activity and Oxidative Stress
- Eicosanoids and Hypertension Pharmacology
- Folate and B Vitamins Research
- Radiopharmaceutical Chemistry and Applications
- Cardiac Imaging and Diagnostics
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Lipoproteins and Cardiovascular Health
- Takotsubo Cardiomyopathy and Associated Phenomena
- Monoclonal and Polyclonal Antibodies Research
- Healthcare Systems and Public Health
- Venous Thromboembolism Diagnosis and Management
- Cardiovascular Issues in Pregnancy
- Glycosylation and Glycoproteins Research
- Cardiac Valve Diseases and Treatments
- Vitamin C and Antioxidants Research
- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Blood properties and coagulation
- Platelet Disorders and Treatments
- Phagocytosis and Immune Regulation
- Cardiovascular Disease and Adiposity
- Pulmonary Hypertension Research and Treatments
Centro Cardiologico Monzino
2014-2023
University of Milan
2006-2022
Istituti di Ricovero e Cura a Carattere Scientifico
2012-2021
The authors hypothesized that the cytokine storm described in COVID-19 patients may lead to consistent cell-based tissue factor (TF)-mediated activation of coagulation, procoagulant microvesicles (MVs) release, and massive platelet activation. have higher levels TF+ platelets, granulocytes, MVs than healthy subjects coronary artery disease patients. Plasma MV-associated thrombin generation is present prophylactic anticoagulated A sustained terms P-selectin expression platelet–leukocyte...
Abstract Background: Oxidative stress is present in cardiovascular diseases (CVDs), and hyperhomocysteinemia, an independent risk factor for these diseases, may play a role by inducing production of oxygen free radicals. Methods: To evaluate the possible homocysteine (Hcy) oxidative coronary artery disease (CAD), plasma Hcy was measured 68 consecutive patients, malondialdehyde (MDA), both total (free + bound), 40 patients with CAD (18 chronic stable angina 22 unstable angina). As controls,...
Reactive oxygen species (ROS) induce nuclear factor erythroid 2–related 2 (Nrf2) activation as an adaptive defense mechanism, determining the synthesis of antioxidant molecules, including heme-oxygenase-1 (HO-1). HO-1 protects cells against oxidative injury, degrading free heme and inhibiting ROS production. is highly expressed in macrophages during plaque growth. Macrophages are morpho-functionally heterogeneous, prevalence a specific phenotype may influence fate. This heterogeneity has...
Background All the enzymatic factors/cofactors involved in nitric oxide (NO) metabolism have been recently found red blood cells. Increased oxidative stress impairs NO bioavailability and has described plasma of coronary artery disease (CAD) patients. The aim study was to highlight a potential dysfunction metabolic profile cells from CAD patients compared with healthy controls. Methods We determined L-arginine/NO pathway by liquid-chromatography tandem mass spectrometry high performance...
Platelet activation is persistently enhanced, and its inhibition by low-dose aspirin impaired in type 2 diabetes mellitus. We investigated vivo thromboxane (TX) prostacyclin (PGI2) biosynthesis their determinants, as well responsiveness, young adult subjects with 1 mellitus (T1DM) without overt cardiovascular disease stable glycemic control. The of TXA2 was increased T1DM versus matched healthy subjects, females showing higher urinary TX metabolite (TXM) excretion than male T1DM....
Development of heart failure depends on systemic and molecular abnormalities among which are the activation neurohormonal systems increase matrix metalloproteinases (MMPs). This study assessed relationship between catecholamines active MMPs in vivo patients with severe congestive (CHF) vitro human cardiac fibroblasts.Forty CHF due to dilated cardiomyopathy, either idiopathic (n=20) or secondary ischaemic disease (n=20), were compared 20 healthy subjects. Plasma MMP-2 MMP-9 activity, but not...
Oxidative stress has been related to various diseases, gender and ageing, measured by markers. The authors developed a procedure compute global oxidative index (OXY-SCORE), reflecting both antioxidant markers in healthy subjects. Its performance was tested relation age coronary artery disease (CAD) patients. Eighty-two subjects 20 CAD patients were enrolled. Plasma free total malondialdehyde (F- T-MDA), glutathione disulphide/reduced form ratio (GSSG/GSH) urine isoprostanes (iPF2alpha-III)...
Abstract Once-daily (od), low-dose aspirin (75–100 mg) is recommended to reduce the thrombotic risk of patients with essential thrombocytemia (ET). This practice based on data extrapolated from other high-risk and an trial in polycythemia vera, assumption similar pharmacodynamics two settings. However, impaired ET, reflecting accelerated renewal platelet cyclooxygenase (COX)-1. ARES a parallel-arm, placebo-controlled, randomized, dose-finding, phase II enrolling 300 ET address main...
Objective: This study examines whether cyclooxygenase 2 (Cox-2) synthesis in human endothelial cells involves different signaling pathways when induced by the proinflammatory cytokine tumor necrosis factor-α (TNFα) or and angiogenic promoter phorbol ester (PMA). Moreover, hypothesis that reactive oxygen species (ROS) an altered redox status within cell are fundamental steps for Cox-2 is verified. Methods: Human isolated from umbilical vein (HUVEC) were exposed to PMA TNFα protein mRNA levels...
Abstract Background Observational studies suggest that low levels of antioxidants are associated with high risk for coronary artery disease (CAD). We investigated whether the biomarkers oxidative balance undergo same modifications in all CAD patient groups, regardless gender and age. Materials methods One hundred sixty‐eight patients 107 healthy controls were assayed plasma reduced glutathione (GSH), α‐ γ‐tocopherol (α‐ γ‐T) as endogenous antioxidants. A damage score (DS), representative...
On-pump cardiac surgery may trigger inflammation and accelerate platelet cyclooxygenase-1 renewal, thereby modifying low-dose aspirin pharmacodynamics. Thirty-seven patients on standard 100 mg once-daily were studied before randomized within 36 hours postsurgery to once-daily, twice-daily, or 200 for 90 days. On day 7 postsurgery, immature mature platelets, mass, thrombopoietin, glycocalicin, leukocytes, C-reactive protein, interleukin-6 significantly increased. Interleukin-6 correlated with...