A5043500953- Cardiac Valve Diseases and Treatments
- Cardiovascular Effects of Exercise
- Tissue Engineering and Regenerative Medicine
- Sports injuries and prevention
- Electrospun Nanofibers in Biomedical Applications
- Infective Endocarditis Diagnosis and Management
- Atherosclerosis and Cardiovascular Diseases
- Infectious Aortic and Vascular Conditions
- Antioxidant Activity and Oxidative Stress
- COVID-19 Clinical Research Studies
- Proteoglycans and glycosaminoglycans research
- Organ Transplantation Techniques and Outcomes
- Cardiac electrophysiology and arrhythmias
- COVID-19 and healthcare impacts
- Cardiac Fibrosis and Remodeling
- Cerebrovascular and Carotid Artery Diseases
- Aortic Disease and Treatment Approaches
- Protease and Inhibitor Mechanisms
- SARS-CoV-2 and COVID-19 Research
- Cardiovascular Function and Risk Factors
- Cardiac Structural Anomalies and Repair
- Cardiac and Coronary Surgery Techniques
- Circular RNAs in diseases
- Protein Interaction Studies and Fluorescence Analysis
- Diphtheria, Corynebacterium, and Tetanus
Centro Cardiologico Monzino
2011-2023
Istituti di Ricovero e Cura a Carattere Scientifico
2011-2022
University of Milan
2001-2013
University of Sassari
2003
University of Chicago
2003
Azienda Socio Sanitaria Territoriale Grande Ospedale Metropolitano Niguarda
1994
Apolipoproteins are very heterogeneous protein family, implicated in plasma lipoprotein structural stabilization, lipid metabolism, inflammation, or immunity. Obtaining detailed information on apolipoprotein composition and structure may contribute to elucidating roles atherogenesis developing new therapeutic strategies for the treatment of lipoprotein-associated disorders. This study aimed at a comprehensive method characterizing component VLDL, LDL, HDL fractions from patients undergoing...
Arrhythmogenic Cardiomyopathy (ACM) is characterized by the replacement of myocardium with fibrotic or fibro-fatty tissue and inflammatory infiltrates in heart. To date, while ACM adipogenesis a well-investigated differentiation program, ACM-related fibrosis remains scientific gap knowledge. In this study, we analyze process occurring during pathogenesis focusing on role cardiac mesenchymal stromal cells (C-MSC) as source myofibroblasts. We performed ex vivo studies plasma right ventricular...
We investigated the presence of antibiotics in cryopreserved cardiovascular tissues and cryopreservation media, after tissue decontamination with antibiotic cocktails, impact residues on standard bank microbiological analyses. Sixteen were decontaminated bank-prepared cocktails by two different banks according to their operating procedures. Before decontamination, samples underwent analysis methods. Cryopreserved tested again without removal using a RESEP tube, thawing. Presence homogenates...
Abstract The research into the pathophysiology of atherosclerosis has considerably increased our understanding disease complexity, but still many questions remain unanswered, both mechanistically and pharmacologically. Here, we provided evidence that pro-oxidant enzyme Prenylcysteine Oxidase 1 (PCYOX1), in human atherosclerotic lesions, is synthesized locally transported within subintimal space by proatherogenic lipoproteins accumulating arterial wall during atherogenesis. Further, Pcyox1...
Based on previous observations apolipoprotein(a), apo(a), in human unstable carotid plaques, we explored whether the inflammatory environment of atheroma, proteolytic events affect other hepatic and topically generated proteins relation to issue plaque stability.Forty 24 stable plaques from endarterectomy segments affected arteries were extracted with buffered saline (PBS) then 6 mol/L guanidine-hydrochloride (GdHCl) identify loosely tightly bound products, respectively. The extracts studied...
Objectives . Oxidative stress has been implicated in the outcome of atherosclerotic plaques. However, at present, no data are available neither on degree plaque protein sulfhydryl groups oxidation nor its relationship with vulnerability. We investigated entity protein-SH oxidative modifications, focusing low molecular weight thiols adduction, human carotid extracts relation to stability/instability. Methods Plaque stability/instability was histologically assessed. The extent modifications...
Abstract Background Cardiac mesenchymal stromal cells (C-MSC) were recently shown to differentiate into adipocytes and myofibroblasts promote the aberrant remodeling of cardiac tissue that characterizes arrhythmogenic cardiomyopathy (ACM). A calcium (Ca 2+ ) signaling dysfunction, mainly demonstrated in mouse models, is recognized as a mechanism impacting arrhythmic risk ACM cardiomyocytes. Whether similar mechanisms influence C-MSC fate still unknown. Thus, we aim ascertain whether...
Objectives. To evaluate if the prooxidant environment present in atherosclerotic plaque may oxidatively modify filtered albumin. Methods. Fluorescein-5-maleimide labelled plasma samples and extracts from 27 patients who had undergone carotid endarterectomy were analysed through nonreducing SDS-PAGE for albumin-Cys 34 oxidation. Furthermore, degree pattern of S-thiolation both circulating plaque-filtered albumin assayed. Results. Albumin showed higher levels Cys oxidative modifications than...
The tracheal reconstruction after wide resections remains a critical surgical problem. Our aim was to replace trachea with tissue easy vascularize, which allows simple and does not require an immunosuppressive regimen.A segment of cryopreserved aorta used in order verify its adequacy as substitute. In phase 1, the thoracic 10 rabbits excised, obtaining 20 segments that were cryopreserved. Ten implanted omentum sacrificed on postoperative days 7, 14 21, grafts examined histologically. 2,...
Abstract: This study was designed to evaluate the effect of cryopreservation on glycosaminoglycan (GAG) content aortic allografts. Twenty‐one porcine valves were obtained. Five roots immediately analyzed without cryopreservation, eight cryopreserved in closed leaflet position, and open position. The groups compared terms GAG concentration subclass proportion three different zones including root wall, commissures, leaflets. at commissures significantly lower group than other ( P = 0.001)....
Several studies have evidenced variations in plasma glycosaminoglycans content physiological and pathological conditions. In normal human GAGs are present mainly as undersulfated chondroitin sulfate (CS). The aim of the study was to evaluate possible correlations between CS level/structure presence/typology carotid atherosclerotic lesion. Plasma purified from 46 control subjects 47 patients undergoing endarterectomy showing either a soft or hard plaque. concentration structural...
In the past years, it has become increasingly clear that protein cargo of different lipoprotein classes is largely responsible for carrying out their various functions, also in relation to pathological conditions, including atherosclerosis. Accordingly, detailed information about apolipoprotein composition and structure may contribute revelation role atherogenesis understanding mechanisms lead atherosclerotic degeneration toward vulnerable plaque formation. With this aim, shotgun proteomics...
Background. Left (LV) and right ventricles (RV) are characterized by well-known physiological differences, mainly related to their different embryological origin, hemodynamic environment, function, structure as well cellular composition. Nevertheless, scarce information is available about peculiarities between left ventricular chambers in pathological contexts. Cardiac mesenchymal stromal cells (C-MSC) key affecting many functions of the heart. Differential features that distinguish LV from...
Human serum albumin (HSA) represents the most abundant plasma protein, with relevant antioxidant activity due to presence of sulfhydryl group on cysteine at position 34 (Cys34), latter being one major target sites for redox-dependent modifications leading formation mixed disulfide linkages low molecular weight thiols. Thiolated forms HSA (Thio-HSA) may be useful as markers an unbalanced redox state and a potential therapeutic target. Indeed, we have previously reported that Cys34 can...
The levels and fine structure of complex polysaccharides, glycosaminoglycans (GAGs), were determined in segments the posterior mitral valve leaflet (MVL) taken from 15 patients affected by regurgitation degenerative disease compared with multiorgan donors.MVL GAGs analyzed agarose gel electrophoresis, HPLC fluorophore-assisted carbohydrate electrophoresis to evaluate disaccharide patterns after treatment chondroitinase ABC.GAGs control group composed approximately 37% hyaluronic acid 63%...