A5035317813- Advanced Proteomics Techniques and Applications
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Advanced Glycation End Products research
- Biochemical effects in animals
- Metabolomics and Mass Spectrometry Studies
- Protein Interaction Studies and Fluorescence Analysis
- Neurological Disorders and Treatments
- Circular RNAs in diseases
- Ferroptosis and cancer prognosis
- Computational Drug Discovery Methods
- Heme Oxygenase-1 and Carbon Monoxide
- Radiomics and Machine Learning in Medical Imaging
- Muscle metabolism and nutrition
- Trace Elements in Health
- Plant biochemistry and biosynthesis
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Drug Transport and Resistance Mechanisms
- Lipoproteins and Cardiovascular Health
- Adipokines, Inflammation, and Metabolic Diseases
- Lipid metabolism and biosynthesis
- Biochemical Acid Research Studies
- Erythropoietin and Anemia Treatment
- Bee Products Chemical Analysis
- Essential Oils and Antimicrobial Activity
- Neonatal Respiratory Health Research
University of Milan
2022-2024
Centro Cardiologico Monzino
2020-2022
Istituti di Ricovero e Cura a Carattere Scientifico
2020-2021
Human serum albumin (HSA) is associated with several physiological functions, such as maintaining oncotic pressure and microvascular integrity, among others. It also represents the major predominant antioxidant in plasma due to presence of Cys34 sulfhydryl group. In this study, we assessed qualitative quantitative changes HSA patients heart failure (HF) their relationship severity disease. We detected by means mass spectrometry a global decrease content HF respect control subjects,...
The lipid profile of skin is fundamental in the maintenance protective barrier against external environment. Signaling and constitutive lipids this large organ are involved inflammation, metabolism, aging, wound healing, such as phospholipids, triglycerides, FFA, sphingomyelin. Skin exposure to ultraviolet (UV) radiation results a photoaging process that an accelerated form aging. UV-A deeply penetrates dermis promotes damage DNA, lipids, proteins by increasing generation reactive oxygen...
Heart failure (HF) is a complex disease due to the intricate interplay of several mechanisms, which therefore implies need for multimarker strategy better personalize care patients with HF. In this study, we developed targeted mass spectrometry approach based on multiple reaction monitoring (MRM) measure circulating protein biomarkers, involved in cardiovascular disease, address their relevance human HF, intending assess feasibility workflow and risk stratification. analyzed total 60 plasma...
Advanced quantitative bioanalytical approaches in combination with network analyses allow us to answer complex biological questions, such as the description of changes protein profiles under disease conditions or upon treatment drugs. In present work, three proteomic approaches-either based on labelling not-in were applied a new vitro cellular model nonalcoholic fatty liver (NAFLD) for first time. This is characterized by accumulation lipids, inflammation, fibrosis, and insulin resistance....
Cigarette smoking is a major independent risk factor for cardiovascular diseases (CVD). The underlying mechanisms, however, are not clearly understood. Lungs the primary route of exposure to smoke, with pulmonary cells and surfactant being first structures directly exposed, resulting in leakage immature proteoform protein B (proSP-B). Herein, we evaluated whether proSP-B joined cargo high-density lipoprotein (HDL) proteins healthy young subjects (n = 106) without any CVD other than smoking,...
Human serum albumin (HSA) represents the most abundant plasma protein, with relevant antioxidant activity due to presence of sulfhydryl group on cysteine at position 34 (Cys34), latter being one major target sites for redox-dependent modifications leading formation mixed disulfide linkages low molecular weight thiols. Thiolated forms HSA (Thio-HSA) may be useful as markers an unbalanced redox state and a potential therapeutic target. Indeed, we have previously reported that Cys34 can...
Advanced glycation end-products (AGEs) and advanced lipoxidation (ALEs), particularly carboxymethyl-lysine (CML), have been largely proposed as factors involved in the establishment progression of heart failure (HF). Despite this evidence, current literature lacks comprehensive identification characterization plasma AGEs/ALEs HF (untargeted approach). This work provides first ex vivo high-resolution mass spectrometry (HR-MS) profiling occurring human serum albumin (HSA), most abundant...
Background: The viral main protease (Mpro) of SARS-CoV-2 has been recently proposed as a key target to inhibit virus replication in the host. Therefore, molecules that can bind catalytic site Mpro could be considered potential drug candidates treatment infections. Here we application state-of-the-art analytical platform which combines metabolomics and protein structure analysis fish-out active compounds deriving from natural matrix, i.e., blueberry extract. Methods: experiments focus on...
The viral 3CL protease of SARS-CoV-2, also called the main (Mpro) has recently been considered as a key target to inhibit virus replication in host. Therefore, molecules that can bind catalytic site Mpro could be potential drug candidates treatment SARS-CoV-2 infections, which so far relies on non-specific drugs. Natural matrices have long source bioactive with wide structural chemical diversity is often difficult reproduce synthetically. Here we proposed an application state art analytical...