A5012845981- Nitric Oxide and Endothelin Effects
- Antioxidant Activity and Oxidative Stress
- Inflammatory mediators and NSAID effects
- Eicosanoids and Hypertension Pharmacology
- Antiplatelet Therapy and Cardiovascular Diseases
- Cerebrovascular and Carotid Artery Diseases
- Hormonal Regulation and Hypertension
- Heart Failure Treatment and Management
- Cardiovascular Issues in Pregnancy
- Cardiac Ischemia and Reperfusion
- Adenosine and Purinergic Signaling
- DNA Repair Mechanisms
- Cardiac, Anesthesia and Surgical Outcomes
- Cancer, Hypoxia, and Metabolism
- Venous Thromboembolism Diagnosis and Management
- Electron Spin Resonance Studies
- Metabolomics and Mass Spectrometry Studies
- Hemoglobin structure and function
- Folate and B Vitamins Research
- Infrared Thermography in Medicine
- Tea Polyphenols and Effects
- Paraoxonase enzyme and polymorphisms
- Cardiac Arrhythmias and Treatments
- Alcohol Consumption and Health Effects
- High Altitude and Hypoxia
Centro Cardiologico Monzino
2009-2019
University of Salford
2016
Istituti di Ricovero e Cura a Carattere Scientifico
2012-2014
Laboratoire de Biochimie
2008
Background All the enzymatic factors/cofactors involved in nitric oxide (NO) metabolism have been recently found red blood cells. Increased oxidative stress impairs NO bioavailability and has described plasma of coronary artery disease (CAD) patients. The aim study was to highlight a potential dysfunction metabolic profile cells from CAD patients compared with healthy controls. Methods We determined L-arginine/NO pathway by liquid-chromatography tandem mass spectrometry high performance...
Platelet activation is persistently enhanced, and its inhibition by low-dose aspirin impaired in type 2 diabetes mellitus. We investigated vivo thromboxane (TX) prostacyclin (PGI2) biosynthesis their determinants, as well responsiveness, young adult subjects with 1 mellitus (T1DM) without overt cardiovascular disease stable glycemic control. The of TXA2 was increased T1DM versus matched healthy subjects, females showing higher urinary TX metabolite (TXM) excretion than male T1DM....
Abstract Background Observational studies suggest that low levels of antioxidants are associated with high risk for coronary artery disease (CAD). We investigated whether the biomarkers oxidative balance undergo same modifications in all CAD patient groups, regardless gender and age. Materials methods One hundred sixty‐eight patients 107 healthy controls were assayed plasma reduced glutathione (GSH), α‐ γ‐tocopherol (α‐ γ‐T) as endogenous antioxidants. A damage score (DS), representative...
OBJECTIVES:We investigated whether oxidative stress and the arginine/nitric oxide pathway differ in control subjects adult patients who are candidates for three most common cardiac surgical operations: coronary bypass surgery, aortic valve replacement calcific non-rheumatic stenosis or mitral repair degenerative insufficiency. METHODS:In this prospective observational study, we studied 165 consecutive undergoing surgery from January to June 2011 (coronary n = 63; stenosis, 51; insufficiency,...
Propofol (2,6-diisopropylphenol) is an anesthetic drug with antioxidant and antiinflammatory properties, documented both in vitro experimental models of ischemia-reperfusion injury septic shock. These properties have been related to the similarity its chemical structure that endogenous tocopherols, which are phenol-containing radical scavengers. This study evaluated effects propofol on alpha- gamma-tocopherol (alpha- gamma-T) levels selected markers oxidant-antioxidant inflammatory status...
Background Mechanisms linking chronic kidney disease (CKD) and adverse outcomes in acute coronary syndromes (ACS) are not fully understood. Among potential key players, reduced nitric oxide (NO) synthesis due to its endogenous inhibitors, asymmetric (ADMA) symmetric (SDMA) dimethylarginine could be involved. We measured plasma concentration of arginine, ADMA SDMA investigated their relationship with CKD long-term outcome non-ST-elevation myocardial infarction (NSTEMI). Methodology/Principal...
Fabrizio Veglia*a, José P. Werbaa, Elena Tremoli, Isabella Squellerioa, Erminio Sisilloa, Alessandro Parolari, Fabiana Minardia & Viviana Cavalcaa Centro Cardiologico Monzino IRCCS, Milan, Italyb Department of Pharmacological Sciences, University Italyc Institute Cardiology, Italy
A decreased nitric oxide (NO) bioavailability and an increased oxidative stress play a pivotal role in different cardiovascular pathologies. As red blood cells (RBCs) participate NO formation the bloodstream, aim of this study was to outline metabolic profile L-arginine (Arg)/NO pathway status RBCs plasma patients with microvascular angina (MVA), investigating similarities differences respect coronary artery disease (CAD) or healthy controls (Ctrl). Analytes involved Arg/NO ratio oxidized...
Summary Essential thrombocythaemia (ET) is characterised by enhanced platelet generation and thrombosis. Once daily (od) aspirin incompletely inhibits thromboxane (TX)A2 production in ET. A twice (bid) dosing necessary to fully inhibit TXA2. Whether this regimen affects vivo prostacyclin (PGI2) biosynthesis unknown. PGI2 was 50 ET patients on enteric-coated (EC) 100 mg od, measuring its urinary metabolite, 2,3-dinor-6-keto-PGF1α (PGI-M). Moreover, a crossover study 22 poorly responsive...
Cigarette smoke (CS) increases the incidence of atherothrombosis, release prostaglandin (PG) E2, and amount tissue factor (TF). The link between PGE2 TF, impact this interaction on CS-induced thrombosis, is unknown. Plasma from active smokers showed higher concentration PGE2, TF total antigen, microparticle-associated (MP-TF) activity compared with never smokers. Similar results were obtained in mice mouse cardiac endothelial cells (MCECs) after treatment aqueous CS extracts (CSEs) plus...
Given to its ability irreversibly acetylate the platelet cyclooxygenase-1 enzyme, acetylsalicylic acid (ASA) is successfully employed for prevention of cardiovascular disease. Recently, an antitumoral effect ASA in colorectal cancer has been increasingly documented. However, molecular and metabolic mechanisms by which exerts such largely unknown. Using a new, untargeted liquid chromatography-mass spectrometry approach, we have analyzed urine samples from seven healthy participants that each...
Coronary artery bypass grafting (CABG), one of the most common cardiac surgical procedures, is characterized by a burst oxidative stress. 8-Oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), produced following DNA repairing, used as an indicator damage in humans. The effect CABG on oxidative-induced damage, evaluated through measurement urinary 8-oxodG developed and validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method 52 coronary disease (CAD) patients, was assessed before...