A5012023050- Cancer, Hypoxia, and Metabolism
- Cancer Cells and Metastasis
- Hippo pathway signaling and YAP/TAZ
- Axon Guidance and Neuronal Signaling
- Protein Tyrosine Phosphatases
- Cell Image Analysis Techniques
- MicroRNA in disease regulation
- Porphyrin and Phthalocyanine Chemistry
- Epigenetics and DNA Methylation
- Cancer, Lipids, and Metabolism
- Luminescence and Fluorescent Materials
- Mitochondrial Function and Pathology
- Photochemistry and Electron Transfer Studies
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Metabolism, Diabetes, and Cancer
- RNA modifications and cancer
- Crystallization and Solubility Studies
- Adipose Tissue and Metabolism
- X-ray Diffraction in Crystallography
- Cancer-related molecular mechanisms research
- Prostate Cancer Treatment and Research
- Organic Light-Emitting Diodes Research
- FOXO transcription factor regulation
- Diet and metabolism studies
- Cancer-related Molecular Pathways
University of Florence
2016-2025
Institute for the Chemistry of OrganoMetallic Compounds
2020-2021
Tumour Institute of Tuscany
2011-2014
Fondazione IRCCS Istituto Nazionale dei Tumori
2012
University of L'Aquila
2011
PharmaMar (Spain)
2005
Abstract Cancer-associated fibroblasts (CAF) engage in tumor progression by promoting the ability of cancer cells to undergo epithelial–mesenchymal transition (EMT), and also enhancing stem traits metastatic dissemination. Here we show that reciprocal interplay between CAFs prostate goes beyond engagement EMT include mutual metabolic reprogramming. Gene expression analysis cultured ex vivo or human obtained from benign hyperplasia revealed Warburg metabolism mitochondrial oxidative stress....
Tumor stromal cells can supply appropriate signals that may develop aggressive phenotypes of carcinoma and establish a complex scenario which culminates in metastasis. Recent works proposed bone marrow-derived mesenchymal stem (MSC) are recruited to primary tumors. However, the exact functions these tumor microenvironment not well characterized, as it is reported MSC either promote or inhibit progression. In present study, we aim at investigating signaling molecules regulate interplay...
Low molecular weight protein tyrosine phosphatase (LMW-PTP) is an enzyme involved in platelet-derived growth factor (PDGF)-induced mitogenesis and cytoskeleton rearrangement because it able to bind dephosphorylate the activated receptor. LMW-PTP presents two cysteines positions 12 17, both belonging catalytic pocket; this a unique feature of among all phosphatases. Our previous results demonstrated that vitro oxidized by either H2O2or nitric oxide with formation disulfide bond between Cys-12...
// Corti Denise 1, 2, * , Paolo Paoli Maura Calvani 1 Maria Letizia Taddei Elisa Giannoni Scott Kopetz 2 Syed Mohammad Ali Kazmi Morelli Pia Piergiorgio Pettazzoni Elena Sacco 3 Anna Caselli Marco Vanoni Matteo Landriscina 4 Cirri Paola Chiarugi Department of Experimental and Clinical Biomedical Sciences, University Florence, Italy Genomic Medicine, The Texas MD Anderson Cancer Center, Houston, Texas, USA SYSBIO Centre for Systems Biology, Biotechnology Biosciences, Milano-Bicocca, Milano,...
// Luigi Ippolito 1 , Alberto Marini 5 Lorenzo Cavallini Andrea Morandi Laura Pietrovito Gianfranco Pintus 5, 6 Elisa Giannoni Thomas Schrader 2 Martin Puhr 3 Paola Chiarugi 1, 4, * Maria Letizia Taddei 7, Department of Experimental and Clinical Biomedical Sciences, University Florence, Italy Chemistry, Duisburg-Essen, Essen, Germany Urology, Medical Innsbruck, Austria 4 Tuscany Tumor Institute “Center for Research, Transfer High Education DenoTHE”, Laboratory Cell Signaling...
We focused our interest on senescent human‐derived fibroblasts in the progression of prostate cancer. Hypoxic promote cancer aggressiveness by inducing epithelial to mesenchymal transition (EMT) and secreting energy‐rich compounds support cell growth. additionally increase: i) recruitment monocytes their M2‐macrophage polarization, ii) bone marrow‐derived endothelial precursor cells, facilitating vasculogenic ability iii) capillary morphogenesis, proliferation invasion human mature cells. In...
We prepared conceptually novel, fully rigid, spiro compact electron donor (Rhodamine B, lactam form, RB)/acceptor (naphthalimide; NI) orthogonal dyad to attain the long-lived triplet charge-transfer (3 CT) state, based on spin control using spin-orbit charge transfer intersystem crossing (SOCT-ISC). Transient absorption (TA) spectra indicate first separation (CS) takes place within 2.5 ps, subsequent SOCT-ISC 8 ns produce 3 NI* state. Then slow secondary CS (125 ns) gives CT state (0.94 μs...
// Elisa Giannoni 1 , Maria Letizia Taddei Andrea Morandi Giuseppina Comito Maura Calvani Francesca Bianchini Barbara Richichi 2 Giovanni Raugei Nicholas Wong 3 Damu Tang Paola Chiarugi Department of Experimental and Clinical Biomedical Sciences, University Florence, 50134, Italy Chemistry, 50019, Sesto Fiorentino, Division Nephrology, Medicine, McMaster University, L8N4A6, Hamilton, Ontario, Canada Correspondence to: Chiarugi, e-mail: paola.chiarugi@unifi.it Keywords: pyruvate kinase M2,...
Aromatase inhibitors (AI) have become the first-line endocrine treatment of choice for postmenopausal estrogen receptor-positive (ER(+)) breast cancer patients, but resistance remains a major challenge. Metabolic reprogramming is hallmark and may contribute to drug resistance. Here, we investigated link between altered metabolism AI using AI-resistant sensitive cells, patient tumor samples, AI-sensitive human xenografts. We found that long-term deprivation (LTED), model resistance, was...
5-Fluorouracil (5-FU) is a key component of chemotherapy for colorectal cancer (CRC). 5-FU efficacy established by intracellular levels folate cofactors and DNA damage repair strategies. However, drug resistance still represents major challenge. Here, we report that alterations in serine metabolism affect sensitivity vitro vivo CRC models. In particular, 5-FU-resistant cells display strong dependency achieved either upregulating endogenous synthesis or increasing exogenous uptake....
Eph tyrosine kinases instruct cell for a repulsive behavior, regulating shape, adhesion, and motility. Beside its role during embryogenesis, neurogenesis, angiogenesis, EphA2 kinase is frequently up-regulated in tumor cells of different histotypes, including prostate, breast, colon, lung carcinoma, as well melanoma. Although function both onset metastasis has been proposed, the played by still debated. Here, we showed that reexpression B16 murine melanoma cells, which use defined mesenchymal...
Stress has an emerging role in cancer and targeting stress-related β-adrenergic receptors (AR) been proposed as a potential therapeutic approach melanoma. Here we report that β3-AR expression correlates with melanoma aggressiveness. In addition, highlight is not only restricted to cells, but it also expressed vivo stromal, inflammatory vascular cells of the microenvironment. Particularly, demonstrated can (i) instruct respond environmental stimuli, (ii) enhance response stromal fibroblasts...
Cellular plasticity confers cancer cells the ability to adapt microenvironmental changes, a fundamental requirement for tumour progression and metastasis. The epithelial mesenchymal transition (EMT) is transcriptional programme associated with increased cell motility stemness. Besides EMT, amoeboid (MAT) has been described during but date, little known about its control involvement in aim of this manuscript investigate (i) profile MAT (ii) study whether acquisition melanoma correlates...
There is growing evidence to suggest that bone marrow‐derived mesenchymal stem cells ( BM ‐ MSC s) are key players in tumour stroma. Here, we investigated the cross‐talk between s and osteosarcoma OS ) cells. We revealed a strong tropism of towards these identified monocyte chemoattractant protein MCP )‐1, growth‐regulated oncogene GRO )‐α transforming growth factor TGF )‐β1 as pivotal factors for chemotaxis. Once contact with cells, trans‐differentiate into cancer‐associated fibroblasts,...
Abstract Background Metabolic reprogramming towards aerobic glycolysis in cancer supports unrestricted cell proliferation, survival and chemoresistance. The molecular bases of these processes are still undefined. Recent reports suggest crucial roles for microRNAs. Here, we provide new evidence the implication miR-27a modulating colorectal (CRC) metabolism Methods A survey expression profile TCGA-COAD dataset revealed that miR-27a-overexpressing CRCs enriched gene signatures mitochondrial...