Alina Bollhagen
A5054469053- Cancer Immunotherapy and Biomarkers
- Advanced Breast Cancer Therapies
- Single-cell and spatial transcriptomics
- Melanoma and MAPK Pathways
- Radiopharmaceutical Chemistry and Applications
- Immune Cell Function and Interaction
- Health, Environment, Cognitive Aging
- Immune cells in cancer
- Cell Image Analysis Techniques
- Delphi Technique in Research
- Cancer Genomics and Diagnostics
- T-cell and B-cell Immunology
- Brain Metastases and Treatment
- Ferroptosis and cancer prognosis
- Immunodeficiency and Autoimmune Disorders
- Immunotherapy and Immune Responses
- Galectins and Cancer Biology
- Hematopoietic Stem Cell Transplantation
- HER2/EGFR in Cancer Research
- IL-33, ST2, and ILC Pathways
- Complement system in diseases
- Breast Cancer Treatment Studies
University of Zurich
2024
ETH Zurich
2024
Life Science Zurich
2024
Massachusetts General Hospital
2023
Brigham and Women's Hospital
2021-2023
Harvard University
2021-2023
Heidelberg Institute for Stem Cell Technology and Experimental Medicine
2022
DKFZ-ZMBH Alliance
2022
Heidelberg University
2021-2022
German Cancer Research Center
2021-2022
<p>Immune cell phenotypes in PT and metastases. <b>A,</b> Uniform Manifold Approximation Projection for Dimension Reduction (UMAP) visualization of all lymphoid cells from the T-cell panel dataset, colored by type (<i>n</i> = 87 patients). <b>B,</b> Heatmap showing mean expression T-cell–relevant markers across different phenotypic subtypes after graph-based clustering patients; <i>n</i>_cells 91,000). Euclidean distance with average...
<p>Overview of the clinical cohort and project workflow. <b>A,</b> Overview experimental The study includes primary breast cancer tissue samples with metastases to four different sites (bone, brain, liver, soft tissue). Three antibody panels were used for IMC on consecutive slides, targeting tumor immune phenotypes. <b>B,</b> Targets three their associated function. <b>C,</b> Proportion indicated cell types per patient in after first graph-based...
<p>Phenotypic composition of tumor cells from PT and distant metastasis. <b>A,</b> Left, heatmap showing the median expression level for each cell cluster after flowSOM clustering. Markers used clustering are on <i>x-</i>axis, clusters <i>y-</i>axis. For clustering, all were pooled across samples (<i>n</i> = 87 patients, 559,953 cells), counts in shown (bar plot, gray). Euclidean distance with Ward-D2 linkage was hierarchical rows. Right,...
<p>Spatial T and myeloid CNs are shared between primary tissue metastatic sites. <b>A,</b> Relative enrichment of T-cell (top) (bottom) subtypes in tumor vs. stromal patches across the whole cohort (<i>n</i> = 87). <b>B,</b> Heatmaps illustrating cell-type frequencies per (left), (middle), or cell (right) neighborhood. Columns <i>z</i>-scored for visualization purposes. The corresponding bubble diagrams show significant results (FDR <...
<div>Abstract<p>Breast cancer is the most commonly diagnosed in women, with distant metastasis being main cause of breast cancer–related deaths. Elucidating changes tumor and immune ecosystems that are associated metastatic disease essential to improve understanding ultimately treatment metastasis. Here, we developed an in-depth, spatially resolved single-cell atlas phenotypic diversity cells primary human tumors matched metastases, using imaging mass cytometry analyze a total 75...
<p>Phenotypic composition analysis of tumor cells between PT and distant metastasis. <b>A,</b> Flow chart depicting the most common phenotypic cluster in vs. metastatic sample. Stacked bar charts show frequency each across patient cohort lines connect matched samples. <b>B,</b> Compositional similarity samples displayed using PCA on per-patient cell abundances as a proportion out all epithelial (abundances were transformed with centered log-ratio...
<p>Immune environment in different metastatic sites and the effect of PT on immune cell composition. <b>A</b> <b>B,</b> T-cell (<b>A</b>) myeloid (<b>B</b>) subtype enrichment or metastasis determined via differential abundance testing using paired design grouped by site metastasis. Bar plot shows log<sub>2</sub>-fold changes (<i>n</i> = 76 patients). <b>C,</b> Boxplot compositional similarity between...
<p>Comparison of the immune environment in PT and metastatic sites. <b>A,</b> Compositional similarity samples displayed using PCA on per-patient cell abundances (<i>n</i> = 76 patients; after applying centered log-ratio transformation). Each point represents a single patient sample either or (M) site. <b>B,</b> Immune type enrichment metastasis determined via differential abundance testing paired design. Bar plot shows...
<p>Supplementary Figures 1-16</p>
Summary: The Imaging and Molecular Annotation of Xenografts Tumors Cancer Grand Challenges team was set up with the objective developing “next generation” pathology cancer research by using a combination single-cell spatial omics tools to produce 3D molecularly annotated maps tumors. Its activities overlapped, in some cases catalyzed, revolution biology that saw new technologies being deployed investigate roles tumor heterogeneity micro-environment. See related article Stratton et al., p. 22...
<p>IMAXT Consortium Author List</p>
<div>Summary:<p>The Imaging and Molecular Annotation of Xenografts Tumors Cancer Grand Challenges team was set up with the objective developing “next generation” pathology cancer research by using a combination single-cell spatial omics tools to produce 3D molecularly annotated maps tumors. Its activities overlapped, in some cases catalyzed, revolution biology that saw new technologies being deployed investigate roles tumor heterogeneity micro-environment.</p><p><a...
<p>IMAXT Consortium Author List</p>
Abstract Breast cancer is the most commonly diagnosed in women, with distant metastasis being main cause of breast cancer-related deaths. Elucidating changes tumor and immune ecosystems that are associated metastatic disease essential to improve understanding ultimately treatment metastasis. Here, we developed an in-depth, spatially resolved single-cell atlas phenotypic diversity cells primary human tumors matched metastases, using imaging mass cytometry analyze a total 75 unique antibody...
Abstract At homeostasis, most Th17 cells are found in the lamina propria of intestine, where they contribute to tissue homeostasis by inhibiting microbiota from invasion and promote barrier functions. Recent studies humans mice have implicated intestinal extra-intestinal autoimmune diseases, including multiple sclerosis, but mechanism which mediate these dichotomous functions remains unknown. Here, we combined single-cell RNA- TCR-seq (scRNA/TCR-seq) with fate mapping profile over 84,000...