Sophia A. Wild
A5103323975- Cancer Genomics and Diagnostics
- Single-cell and spatial transcriptomics
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- RNA Research and Splicing
- Melanoma and MAPK Pathways
- Health, Environment, Cognitive Aging
- Protein Degradation and Inhibitors
- Digestive system and related health
- Evolution and Genetic Dynamics
- Liver Disease Diagnosis and Treatment
- Liver physiology and pathology
- Delphi Technique in Research
- CRISPR and Genetic Engineering
- Protein Tyrosine Phosphatases
- RNA and protein synthesis mechanisms
- Gastrointestinal motility and disorders
- Gene expression and cancer classification
- Bioinformatics and Genomic Networks
- Liver Diseases and Immunity
- ATP Synthase and ATPases Research
- Molecular Biology Techniques and Applications
- Genomics and Phylogenetic Studies
- PI3K/AKT/mTOR signaling in cancer
- Cell Image Analysis Techniques
Novartis (Switzerland)
2024-2025
University of Cambridge
2017-2023
Cancer Research UK
2019-2023
Vanderbilt University Medical Center
2022
University of Zurich
2022
Cancer Research UK Cambridge Center
2017-2022
University of British Columbia
2019
BC Cancer Agency
2017-2018
German Cancer Research Center
2017
Heidelberg University
2017
Accurate measurement of clonal genotypes, mutational processes, and replication states from individual tumor-cell genomes will facilitate improved understanding tumor evolution. We have developed DLP+, a scalable single-cell whole-genome sequencing platform implemented using commodity instruments, image-based object recognition, open source computational methods. Using we generated resource 51,926 matched cell images diverse types including lines, xenografts, diagnostic samples with limited...
How cell-to-cell copy number alterations that underpin genomic instability
Synthetic lethality results when mutant KRAS and EGFR proteins are co-expressed in human lung adenocarcinoma (LUAD) cells, revealing the biological basis for mutual exclusivity of mutations. We have now defined biochemical events responsible toxic effects by combining pharmacological genetic approaches to show that signaling through extracellular signal-regulated kinases (ERK1/2) mediates toxicity. These findings imply tumors with oncogenes RAS pathway must restrain activity ERK1/2 avoid...
Article Figures and data Abstract Introduction Results Discussion Materials methods Data availability References Decision letter Author response author information Metrics A substantial fraction of the genome is transcribed in a cell-type-specific manner, producing long non-coding RNAs (lncRNAs), rather than protein-coding transcripts. Here, we systematically characterize transcriptional dynamics during hematopoiesis hematological malignancies. Our analysis annotated de novo assembled...
Vasculogenic mimicry (VM) describes the formation of pseudo blood vessels constructed tumor cells that have acquired endothelial-like properties. VM channels endow with a tumor-derived vascular system directly connects to host vessels, and their presence is generally associated poor patient prognosis. Here we show transcription factor, Foxc2, promotes in diverse solid types by driving ectopic expression endothelial genes cells, process stimulated hypoxia. VM-proficient tumors are resistant...
Long non-coding RNAs (lncRNAs) show patterns of tissue- and cell type-specific expression that are very similar to those protein coding genes consequently have the potential control stem progenitor fate decisions along a differentiation trajectory. To understand roles lncRNAs may play in hematopoiesis, we selected subset mouse with potentially relevant refined our candidate list using evidence conserved human blood lineages. For each candidate, assessed its possible role hematopoietic vivo...
Summary: The Imaging and Molecular Annotation of Xenografts Tumors Cancer Grand Challenges team was set up with the objective developing “next generation” pathology cancer research by using a combination single-cell spatial omics tools to produce 3D molecularly annotated maps tumors. Its activities overlapped, in some cases catalyzed, revolution biology that saw new technologies being deployed investigate roles tumor heterogeneity micro-environment. See related article Stratton et al., p. 22...
<p>IMAXT Consortium Author List</p>
<div>Summary:<p>The Imaging and Molecular Annotation of Xenografts Tumors Cancer Grand Challenges team was set up with the objective developing “next generation” pathology cancer research by using a combination single-cell spatial omics tools to produce 3D molecularly annotated maps tumors. Its activities overlapped, in some cases catalyzed, revolution biology that saw new technologies being deployed investigate roles tumor heterogeneity micro-environment.</p><p><a...
<p>IMAXT Consortium Author List</p>
Metabolic dysfunction-associated steatotic liver disease (MASLD)—characterized by excess accumulation of fat in the liver—now affects one third world's population. As MASLD progresses, extracellular matrix components including collagen accumulate causing tissue fibrosis, a major determinant severity and mortality. To identify transcriptional regulators we computationally inferred activity transcription factors (TFs) relevant to fibrosis profiling matched transcriptomes epigenomes 108 human...
Abstract Non-alcoholic fatty liver disease (NAFLD) - characterized by excess accumulation of fat in the now affects one third world’s population. As NAFLD progresses, extracellular matrix components including collagen accumulate causing tissue fibrosis, a major determinant severity and mortality. To identify transcriptional regulators we computationally inferred activity transcription factors (TFs) relevant to fibrosis profiling matched transcriptomes epigenomes 108 human biopsies from...
Tumour heterogeneity is thought to be a major barrier successful cancer treatment due the presence of drug resistant clonal lineages. However, identifying characteristics such lineages that underpin resistance therapy has remained challenging. Here, we utilise transcriptomics with WILD-seq;
Drug-induced gastrointestinal toxicity is a frequent clinical adverse event that needs to be carefully monitored and managed ensure patient compliance. While preclinical assessment of drug-induced mostly relies on animal experimentation, intestinal organoids have gained increasing attention identify toxicants in vitro. Nonetheless, current vitro protocols primarily assess structural alterations induced by drugs, whereas events can often stem from functional disturbances. Disruption serotonin...
Abstract We recently described the synthetic lethality that results when mutant KRAS and EGFR are coexpressed in human lung adenocarcinoma (LUAD) cells, revealing biological basis for mutual exclusivity of mutations cancers. have now further defined biochemical events responsible toxic effects signaling through RAS pathway. By combining pharmacological genetic approaches, we developed multiple lines evidence extracellular signal-regulated kinases (ERK1/2) mediates toxicity. These findings...
Summary Long non-coding RNAs (lncRNAs) show patterns of tissue- and cell-type-specific expression that are very similar to those protein coding genes consequently have the potential control stem progenitor cell fate decisions along a differentiation trajectory. To understand roles lncRNAs might play in hematopoiesis, we selected subset mouse with potentially relevant refined our candidate list using evidence conserved human blood lineages. For each candidate, assessed its possible role...
Abstract Tumor heterogeneity is thought to be a major barrier successful cancer treatment due the presence of drug resistant clonal lineages. However, identifying characteristics such lineages that underpin resistance therapy has remained challenging. Here we utilize transcriptomics with WILD-seq; W holistic I nterrogation L ineage D ynamics by seq uencing, in mouse models triple-negative breast (TNBC) understand response and therapy, including BET bromodomain inhibition taxane-based...