Marcel Burger
A5067917124- Analytical chemistry methods development
- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- Mass Spectrometry Techniques and Applications
- Laser-induced spectroscopy and plasma
- Health, Environment, Cognitive Aging
- Geochemistry and Geologic Mapping
- Evolution and Genetic Dynamics
- Ion-surface interactions and analysis
- Geological and Geochemical Analysis
- Delphi Technique in Research
- Cell Image Analysis Techniques
- Mineralogy and Gemology Studies
- Cultural Heritage Materials Analysis
- Geology and Paleoclimatology Research
- Advanced X-ray and CT Imaging
- CRISPR and Genetic Engineering
- Mineral Processing and Grinding
- Cancer Cells and Metastasis
- Image Processing and 3D Reconstruction
- Genomics and Phylogenetic Studies
- Advanced Fluorescence Microscopy Techniques
- Metal Extraction and Bioleaching
- RNA and protein synthesis mechanisms
- Hydrocarbon exploration and reservoir analysis
University of Zurich
2021-2022
ETH Zurich
2014-2021
Charles Humbert 8
2017-2018
Identifying transcript location in cells where specific RNAs occur within a cell or tissue has been limited by technology and imaging capabilities. Expansion microscopy allowed for better visualization of small structures expanding the tissues with polymer- hydrogel-based system. Alon et al. combined expansion long-read situ RNA sequencing, resulting more precise transcripts. This method, termed “ExSeq” was used to detect RNAs, both new transcripts those previously demonstrated localize...
Accurate measurement of clonal genotypes, mutational processes, and replication states from individual tumor-cell genomes will facilitate improved understanding tumor evolution. We have developed DLP+, a scalable single-cell whole-genome sequencing platform implemented using commodity instruments, image-based object recognition, open source computational methods. Using we generated resource 51,926 matched cell images diverse types including lines, xenografts, diagnostic samples with limited...
Abstract A holistic understanding of tissue and organ structure function requires the detection molecular constituents in their original three-dimensional (3D) context. Imaging mass cytometry (IMC) enables simultaneous up to 40 antigens transcripts using metal-tagged antibodies but has so far been restricted two-dimensional imaging. Here we report development 3D IMC for multiplexed analysis at single-cell resolution demonstrate utility technology by human breast cancer samples. The resulting...
How cell-to-cell copy number alterations that underpin genomic instability
Summary: The Imaging and Molecular Annotation of Xenografts Tumors Cancer Grand Challenges team was set up with the objective developing “next generation” pathology cancer research by using a combination single-cell spatial omics tools to produce 3D molecularly annotated maps tumors. Its activities overlapped, in some cases catalyzed, revolution biology that saw new technologies being deployed investigate roles tumor heterogeneity micro-environment. See related article Stratton et al., p. 22...
Low-dispersion laser ablation (LA) has been combined with inductively coupled plasma-time-of-flight mass spectrometry (ICP-TOFMS) to provide full-spectrum elemental imaging at high lateral resolution and fast image-acquisition speeds. The low-dispersion LA cell reported here is capable of delivering 99% the total signal within 9 ms, prototype TOFMS instrument enables simultaneous representative determination all ions from these fast-transient events. This ablated-aerosol transport eliminates...
Here we describe the capabilities of laser-ablation coupled to inductively plasma time-of-flight mass spectrometry (LA-ICP-TOFMS) for high-speed, high-resolution, quantitative three-dimensional (3D) multielemental imaging. The basic operating principles this instrumental setup and a verification 3D elemental imaging are provided. To demonstrate potential LA-ICP-TOFMS imaging, high-resolution multielement images cesium-infiltrated Opalinus clay rock were recorded using LA with laser-spot...
The heterogeneity of breast cancer plays a major role in drug response and resistance has been extensively characterized at the genomic level. Here, single-cell mass cytometry (BCMC) panel is optimized to identify cell phenotypes their oncogenic signalling states biobank patient-derived tumour xenograft (PDTX) models representing diversity human cancer. BCMC identifies 13 cellular (11 2 murine), associated with both subtypes specific features. Pre-treatment phenotypic composition determinant...
Evaluation of capabilities offered by ICP-TOFMS for various laser-ablation-based sample introduction schemes.
<p>IMAXT Consortium Author List</p>
<div>Summary:<p>The Imaging and Molecular Annotation of Xenografts Tumors Cancer Grand Challenges team was set up with the objective developing “next generation” pathology cancer research by using a combination single-cell spatial omics tools to produce 3D molecularly annotated maps tumors. Its activities overlapped, in some cases catalyzed, revolution biology that saw new technologies being deployed investigate roles tumor heterogeneity micro-environment.</p><p><a...
<p>IMAXT Consortium Author List</p>
High time resolution of TOFMS allows to study variations in ion transit times caused by collisions within the pressurized cell.
A public demonstration of laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS) for fast and sensitive qualitative elemental analysis solid everyday objects is described. This served as a showcase model modern instrumentation (and analysis, in particular) to the public. Several steps were made adapt conventional laboratory-based ICP-MS bring it outreach event also improve ease-of-use, which helped facilitate discussion with attendees. Rather than closed-cell design, this...
Inductively coupled plasma mass spectrometry is increasingly used for non-traditional applications such as the analysis of solids at high spatial resolution when combined with laser ablation or engineered nanoparticles. This report highlights recent projects and discusses potentials limitations these techniques offer. High-resolution instrumentation allows element imaging ?m-scale can, therefore, be applied to, e.g., mapping metal isotope-labeled antibodies in biological tissues. Despite...
This data release provides descriptions of more than 100 mining districts, mines, and mineral occurrences (deposits prospects) within the United States that are reported to contain enrichments rhenium (Re). These include mined deposits, exploration prospects, other with notable concentrations rhenium. The inclusion a particular occurrence in this database is not meant imply it has economic potential. Rather, these were included capture distribution characteristics known, States. Rhenium one...
&lt;p&gt;Garnet is one of the most robust and ubiquitous minerals that record element zoning during crustal metamorphism. In addition to major distribution, in trace elements can provide a wealth information document changing conditions garnet growth modification. Trace distribution grains was mapped 2D thin section with laser ablation inductively coupled plasma time flight mass spectrometry (LA-ICP-TOFMS) conventional LA-ICP-MS achieve lateral resolution 15-5 &amp;#181;m limits...