Teresa Ruíz
A5029825875- Enzyme Structure and Function
- Advanced Electron Microscopy Techniques and Applications
- ATP Synthase and ATPases Research
- Electron and X-Ray Spectroscopy Techniques
- Bacteriophages and microbial interactions
- Streptococcal Infections and Treatments
- Oral microbiology and periodontitis research
- Photosynthetic Processes and Mechanisms
- Bacterial Genetics and Biotechnology
- Fungal and yeast genetics research
- Mitochondrial Function and Pathology
- Bacterial biofilms and quorum sensing
- RNA and protein synthesis mechanisms
- Infective Endocarditis Diagnosis and Management
- Genomics and Phylogenetic Studies
- Microbial Metabolic Engineering and Bioproduction
- Microbial Metabolites in Food Biotechnology
- Plant nutrient uptake and metabolism
- Advanced Materials Characterization Techniques
- Protein Structure and Dynamics
- Biochemical and Structural Characterization
- Advanced X-ray Imaging Techniques
- Enzyme Catalysis and Immobilization
- Enzyme Production and Characterization
- Metabolism, Diabetes, and Cancer
University of Vermont
2012-2024
Universitat Autònoma de Barcelona
2024
Hospital Universitario Ramón y Cajal
2023
Engineering Arts (United States)
2021
University of British Columbia
2019
Bruker (United States)
2014
Cornell University
2014
Micron (United States)
2014
EA Technology
2014
Thermo Fisher Scientific (United States)
2014
Identifying transcript location in cells where specific RNAs occur within a cell or tissue has been limited by technology and imaging capabilities. Expansion microscopy allowed for better visualization of small structures expanding the tissues with polymer- hydrogel-based system. Alon et al. combined expansion long-read situ RNA sequencing, resulting more precise transcripts. This method, termed “ExSeq” was used to detect RNAs, both new transcripts those previously demonstrated localize...
Accurate measurement of clonal genotypes, mutational processes, and replication states from individual tumor-cell genomes will facilitate improved understanding tumor evolution. We have developed DLP+, a scalable single-cell whole-genome sequencing platform implemented using commodity instruments, image-based object recognition, open source computational methods. Using we generated resource 51,926 matched cell images diverse types including lines, xenografts, diagnostic samples with limited...
Summary: The Imaging and Molecular Annotation of Xenografts Tumors Cancer Grand Challenges team was set up with the objective developing “next generation” pathology cancer research by using a combination single-cell spatial omics tools to produce 3D molecularly annotated maps tumors. Its activities overlapped, in some cases catalyzed, revolution biology that saw new technologies being deployed investigate roles tumor heterogeneity micro-environment. See related article Stratton et al., p. 22...
The serine-rich repeat family of fimbriae play important roles in the pathogenesis streptococci and staphylococci. Despite recent attention, their finer structural details precise adhesion mechanisms have yet to be determined. Fap1 (Fimbriae-associated protein 1) is major subunit from Streptococcus parasanguinis plays an essential role fimbrial biogenesis, adhesion, early stages dental plaque formation. Combining multidisciplinary, high resolution studies with biological assays, we provide...
The three-dimensional structure of the Manduca sexta midgut V1 ATPase has been determined at 3.2 nm resolution from electron micrographs negatively stained specimens. complex a barrel-like 11 in height and 13.5 diameter. It is hexagonal top view, whereas side six large subunits A B are interdigitated for most their length (9 nm). topology importance individual have explored by protease digestion, resistance to chaotropic agents, MALDI-TOF mass spectrometry, CuCl2-induced disulfide formation....
<p>IMAXT Consortium Author List</p>
<div>Summary:<p>The Imaging and Molecular Annotation of Xenografts Tumors Cancer Grand Challenges team was set up with the objective developing “next generation” pathology cancer research by using a combination single-cell spatial omics tools to produce 3D molecularly annotated maps tumors. Its activities overlapped, in some cases catalyzed, revolution biology that saw new technologies being deployed investigate roles tumor heterogeneity micro-environment.</p><p><a...
<p>IMAXT Consortium Author List</p>
Hydrogenase and fumarate reductase isolated from Wolinella succinogenes were incorporated into liposomes containing menaquinone. The two enzymes found to be oriented solely the outside of resulting proteoliposomes. proteoliposomes catalyzed reduction by H2 which generated an electrical proton potential (Delta(psi) = 0.19 V, negative inside) in same direction as that respiration cells W. succinogenes. H+/e ratio brought about with presence valinomycin external K+ was approximately 1....
Actinobacillus actinomycetemcomitans is a gram-negative, facultative, anaerobic bacterium that colonizes the human oral cavity and upper respiratory tract. This strongly associated with localized aggressive periodontitis adult causative agent for other serious systemic infections. Recently, we have identified protein, EmaA (extracellular matrix protein adhesin A), mediates adhesion of A. to collagen. The conserved sequence predicted secondary structure suggest an orthologue Yersinia...
Gram-negative bacteria display either a flat or an irregular outer membrane. The periodontal pathogen Aggregatibacter (Actinobacillus) actinomycetemcomitans has We have identified gene that is associated with the biogenesis of this morphology. part three-gene operon and codes for 141-kDa protein designated morphogenesis C (MorC), which conserved in several gram-negative including Haemophilus influenzae Pasteurella multocida. Insertional inactivation resulted conversion irregularly shaped...
Background/aims: Streptococcus parasanguinis is a primary colonizer of the tooth surface. Its adhesion mediated by long fimbriae, which are composed multiple subunits serine‐rich glycoprotein, Fap1. Previous studies revealed that chromosomal region located downstream fap1 involved in secretion and glycosylation In this study, we investigated role glycosylation‐associated gene, gap3 , Fap1 biogenesis. Methods: A non‐polar mutant was constructed insertional inactivation. The phenotype...
Extracellular matrix protein adhesin A (EmaA) is a 202-kDa nonfimbrial adhesin, which mediates the adhesion of oral pathogen Aggregatibacter actinomycetemcomitans to collagen. EmaA oligomers form surface antenna-like protrusions consisting long helical rod with an ellipsoidal ending. The functional analysis in-frame emaA deletion mutants has located collagen binding activity amino terminus corresponding acids 70 386. level this mutant was comparable strain. Transmission electron microscopy...
ABSTRACT Aggregatibacter actinomycetemcomitans is hypothesized to colonize through the interaction with collagen and establish a reservoir for further dissemination. The trimeric adhesin EmaA of A. binds modified sugars mediated by an O-antigen polysaccharide ligase (WaaL) that associated lipopolysaccharide (LPS) biosynthesis (G. Tang K. Mintz, J. Bacteriol. 192: 1395–1404, 2010). This investigation characterized function cellular localization glycosylation. interruption LPS biogenesis using...
Dental biofilm formation is critical for maintaining the healthy microbial ecology of oral cavity. Streptococci are predominant bacterial species in cavity and play important roles initiation plaque formation. In this study, we identified a new cell surface protein, BapA1, from Streptococcus parasanguinis FW213 determined that BapA1 Sequence analysis revealed possesses typical wall-sorting signal surface-anchored proteins Gram-positive bacteria. No functional orthologue was reported other...