A5055221489- Identification and Quantification in Food
- Genomics and Phylogenetic Studies
- Microbial infections and disease research
- Environmental DNA in Biodiversity Studies
- Cancer Genomics and Diagnostics
- Molecular Biology Techniques and Applications
- Single-cell and spatial transcriptomics
- Gut microbiota and health
- melanin and skin pigmentation
- Delphi Technique in Research
- Cancer Research and Treatments
- Aquaculture disease management and microbiota
- Tuberculosis Research and Epidemiology
- Prenatal Substance Exposure Effects
- Gene expression and cancer classification
- Folate and B Vitamins Research
- Diet and metabolism studies
- Renal and related cancers
- RNA modifications and cancer
- Microbial Community Ecology and Physiology
- Cutaneous Melanoma Detection and Management
- Epigenetics and DNA Methylation
- Mycobacterium research and diagnosis
- Fatty Acid Research and Health
- Metabolism and Genetic Disorders
South African Medical Research Council
2023-2024
Stellenbosch University
2021-2024
University of British Columbia
2018-2024
Florida International University
2014-2016
National Institutes of Health
2012
Center for Scientific Review
2012
Eisenhower Medical Center
2012
Ottawa Hospital
2012
Ottawa Hospital Research Institute
2012
Reproductive Science Center
2012
Accurate measurement of clonal genotypes, mutational processes, and replication states from individual tumor-cell genomes will facilitate improved understanding tumor evolution. We have developed DLP+, a scalable single-cell whole-genome sequencing platform implemented using commodity instruments, image-based object recognition, open source computational methods. Using we generated resource 51,926 matched cell images diverse types including lines, xenografts, diagnostic samples with limited...
Animal tuberculosis is a significant infectious disease affecting both livestock and wildlife populations worldwide. Effective surveillance characterization of Mycobacterium bovis (M. bovis) strains are essential for understanding transmission dynamics implementing control measures. Currently, sequencing genomic information has relied on culture-based methods, which time-consuming, resource-demanding, concerning in terms biosafety. This study explores the use culture-independent long-read...
Summary Synovial sarcoma is an aggressive soft-tissue malignancy that characterized by a pathognomonic t(X;18)(p11.2;q11.2) translocation, which produces the fusion oncogene named SS18::SSX . Despite recent advancements in our understanding of synovial biology, cell-of-origin remains undefined. A mesenchymal stromal cell (MSC) specific CreERT2 line was employed to express fibroblasts and related types, resulting 100% penetrant development mice, with median latency period 16.2 ± 2.5 weeks....
Summary Essential features of cancer tissue cellular heterogeneity such as negatively selected genome topologies, sub-clonal mutation patterns and replication states can only effectively be studied by sequencing single-cell genomes at scale high fidelity. Using an amplification-free approach implemented on commodity hardware (DLP+) coupled with a cloud-based computational platform, we define resource 40,000 characterized their states, across wide range types conditions. We show that shallow...
Ecological studies of microbial communities often use profiling methods but the true community diversity can be underestimated in that separate amplicons based on sequence length using performance optimized polymer 4. Taxonomically, unrelated organisms produce same amplicon even though have different sequences. F-108 has previously been shown to resolve by polymorphisms. In this study, we showed polymer, ABI Prism 310 Genetic Analyzer and CE, resolved four bacteria produced for 16S rRNA...
Molecular methods for the detection of mammalian coat color phenotypes have expanded greatly within past decade. Many are associated with a single nucleotide polymorphism mutation in genetic sequence. Traditionally, these mutations detected through sequencing, hybridization assays or mini-sequencing. However, techniques can be expensive and tedious. Previously, CE-SSCP using F-108 polymer was able to distinguish SNPs melanocortin-1 receptor (mc1r) gene horses (Equus caballus) that differed...
Hypervariable region sequencing of the 16S ribosomal RNA (rRNA) gene plays a critical role in microbial ecology by offering insights into bacterial communities within specific niches. While providing valuable genus-level information, its reliance on data from targeted genetic regions limits overall utility. Recent advances technologies have enabled characterisation full-length rRNA gene, enhancing species-level classification. Although current short-read platforms are cost-effective and...
Abstract Hypervariable region sequencing of the 16S rRNA gene plays a critical role in microbial ecology, offering insights into bacterial communities within specific niches. While providing valuable genuslevel information, its reliance on limited genetic data from targeted regions this limits overall utility. Advances have enabled characterisation full-length gene, enhancing species-level classification. Third-generation technologies can sequence genes single reads, yet challenges cost and...