Sandra Tietscher

ORCID: 0000-0001-9613-2224
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About
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Research Areas
  • Cancer Immunotherapy and Biomarkers
  • Single-cell and spatial transcriptomics
  • Cancer Genomics and Diagnostics
  • Advanced Breast Cancer Therapies
  • Melanoma and MAPK Pathways
  • Radiopharmaceutical Chemistry and Applications
  • Immune cells in cancer
  • Cancer Cells and Metastasis
  • Cell Image Analysis Techniques
  • Advanced Proteomics Techniques and Applications
  • CRISPR and Genetic Engineering
  • Breast Cancer Treatment Studies
  • Brain Metastases and Treatment
  • Medical Imaging Techniques and Applications
  • Marketing and Advertising Strategies
  • Evolution and Genetic Dynamics
  • Polish socio-economic development
  • Management and Organizational Practices
  • Escherichia coli research studies
  • RNA and protein synthesis mechanisms
  • Ferroptosis and cancer prognosis
  • Advanced Fluorescence Microscopy Techniques
  • HER2/EGFR in Cancer Research
  • Cancer, Stress, Anesthesia, and Immune Response
  • Antibiotic Resistance in Bacteria

University of Zurich
2020-2024

ETH Zurich
2021-2022

Life Science Zurich
2021-2022

IIT@MIT
2021

McGovern Institute for Brain Research
2021

Engineering Arts (United States)
2021

Allen Institute
2021

Howard Hughes Medical Institute
2021

Uppsala University
2018

10.1038/s43018-020-0026-6 article EN Nature Cancer 2020-02-17
Emma Laks Andrew McPherson Hans Zahn Daniel Lai Adi Steif and 95 more Jazmine Brimhall Justina Biele Beixi Wang Tehmina Masud Jerome Ting Diljot Grewal Cydney Nielsen Samantha Leung Viktoria Bojilova Maia A. Smith Oleg Golovko Steven S.S. Poon Peter Eirew Farhia Kabeer Teresa Ruiz de Algara So Ra Lee M. Jafar Taghiyar Curtis Huebner Jessica Ngo Tim Hon Man Chan Spencer Vatrt-Watts Pascale Walters Nafis Abrar Sophia Chan Matt Wiens Lauren Martin R. Wilder Scott T. Michael Underhill Elizabeth A. Chavez Christian Steidl Daniel Da Costa Yussanne Ma Robin Coope Richard Corbett Stephen Pleasance Richard A. Moore Andrew J. Mungall Colin Mar Fergus Cafferty Karen A. Gelmon Stephen Chia Marco A. Marra Carl L. Hansen Sohrab P. Shah Samuel Aparício Gregory J. Hannon Giorgia Battistoni Dario Bressan Ian G. Cannell Hannah Casbolt Cristina Jauset Tatjana Kovačević Claire M. Mulvey Fiona Nugent Marta Ribes Isabella Pearsall Fatime Qosaj Kirsty Sawicka Sophia A. Wild Elena Williams Samuel Aparício Emma Laks Yangguang Li Ciara H. O’Flanagan Austin Smith Teresa Ruíz Shankar Balasubramanian Maximillian Lee Bernd Bodenmiller Marcel Burger Laura Kuett Sandra Tietscher Jonas Windager Edward S. Boyden Shahar Alon Yi Cui Amauche Emenari Dan Goodwin Emmanouil D. Karagiannis Anubhav Sinha Asmamaw T. Wassie Carlos Caldas Alejandra Bruna Maurizio Callari Wendy Greenwood Giulia Lerda Yaniv Lubling Alastair Marti Oscar M. Rueda Abigail Shea Owen Harris Robby Becker Flaminia Grimaldi Suvi Harris Sara Lisa Vogl

Accurate measurement of clonal genotypes, mutational processes, and replication states from individual tumor-cell genomes will facilitate improved understanding tumor evolution. We have developed DLP+, a scalable single-cell whole-genome sequencing platform implemented using commodity instruments, image-based object recognition, open source computational methods. Using we generated resource 51,926 matched cell images diverse types including lines, xenografts, diagnostic samples with limited...

10.1016/j.cell.2019.10.026 article EN cc-by-nc-nd Cell 2019-11-01

Abstract A holistic understanding of tissue and organ structure function requires the detection molecular constituents in their original three-dimensional (3D) context. Imaging mass cytometry (IMC) enables simultaneous up to 40 antigens transcripts using metal-tagged antibodies but has so far been restricted two-dimensional imaging. Here we report development 3D IMC for multiplexed analysis at single-cell resolution demonstrate utility technology by human breast cancer samples. The resulting...

10.1038/s43018-021-00301-w article EN cc-by Nature Cancer 2021-12-24

Cancer-associated fibroblasts (CAFs) are a diverse cell population within the tumour microenvironment, where they have critical effects on evolution and patient prognosis. To define CAF phenotypes, we analyse single-cell RNA sequencing (scRNA-seq) dataset of over 16,000 stromal cells from tumours 14 breast cancer patients, based which functionally annotate nine phenotypes one class pericytes. We validate this classification system in four additional types use highly multiplexed imaging mass...

10.1038/s41467-023-39762-1 article EN cc-by Nature Communications 2023-07-18

Abstract Immune checkpoint therapy in breast cancer remains restricted to triple negative patients, and long-term clinical benefit is rare. The primary aim of immune blockade prevent or reverse exhausted T cell states, but exhaustion tumors not well understood. Here, we use single-cell transcriptomics combined with imaging mass cytometry systematically study environments human that either do contain cells, a focus on luminal subtypes. We find the presence PD-1 high exhaustion-like phenotype...

10.1038/s41467-022-35238-w article EN cc-by Nature Communications 2023-01-06
Sohrab Salehi Farhia Kabeer Nicholas Ceglia Mirela Andronescu Marc Williams and 95 more Kieran R. Campbell Tehmina Masud Beixi Wang Justina Biele Jazmine Brimhall David Gee Hakwoo Lee Jerome Ting Allen W. Zhang Hoa Tran Ciara H. O’Flanagan Fatemeh Dorri Nicole Rusk Teresa Ruiz de Algara So Ra Lee Brian Yu Chieh Cheng Peter Eirew Takako Kono Jenifer Pham Diljot Grewal Daniel Lai Richard A. Moore Andrew J. Mungall Marco A. Marra Gregory J. Hannon Giorgia Battistoni Dario Bressan Ian G. Cannell Hannah Casbolt Atefeh Fatemi Cristina Jauset Tatjana Kovačević Claire M. Mulvey Fiona Nugent Marta Ribes Isabella Pearsall Fatime Qosaj Kirsty Sawicka Sophia A. Wild Elena Williams Emma Laks Yangguang Li Ciara H. O’Flanagan Austin Smith Teresa Ruíz Daniel Lai Andrew Roth Shankar Balasubramanian Maximillian Lee Bernd Bodenmiller Marcel Burger Laura Kuett Sandra Tietscher Jonas Windhager Edward S. Boyden Shahar Alon Yi Cui Amauche Emenari Dan Goodwin Emmanouil D. Karagiannis Anubhav Sinha Asmamaw T. Wassie Carlos Caldas Alejandra Bruna Maurizio Callari Wendy Greenwood Giulia Lerda Yaniv Eyal-Lubling Oscar M. Rueda Abigail Shea Owen Harris Robby Becker Flaminia Grimaldi Suvi Harris Sara Lisa Vogl Joanna Weselak Johanna A. Joyce Spencer S. Watson Ignacio Vázquez-Garćıa Simon Tavaré Khanh N. Dinh Eyal Fisher Russell Kunes N. A. Walton Mohammad Al Sa’d Nick Chornay A. Dariush E. A. González-Solares Carlos González‐Fernández A. Yoldaş Neil S. Millar Tristan Whitmarsh Xiaowei Zhuang Jean Fan Hsuan Lee

10.1038/s41586-021-03648-3 article EN Nature 2021-06-23
Tyler Funnell Ciara H. O’Flanagan Marc Williams Andrew McPherson Steven McKinney and 95 more Farhia Kabeer Hakwoo Lee Sohrab Salehi Ignacio Vázquez-Garćıa Hongyu Shi Emily L Leventhal Tehmina Masud Peter Eirew Damian Yap Allen W. Zhang Jamie Lim Beixi Wang Jazmine Brimhall Justina Biele Jerome Ting Vinci Au Michael Van Vliet Yifei Liu Sean Beatty Daniel Lai Jenifer Pham Diljot Grewal Douglas N. Abrams Eliyahu Havasov Samantha Leung Viktoria Bojilova Richard A. Moore Nicole Rusk Florian Uhlitz Nicholas Ceglia Adam C. Weiner Elena Zaikova J. Maxwell Douglas Dmitriy Zamarin Britta Weigelt Sarah H. Kim Arnaud Da Cruz Paula Jorge S. Reis‐Filho Spencer D. Martin Yangguang Li Hongxia Xu Teresa Ruiz de Algara So Ra Lee Viviana Cerda Llanos David G. Huntsman Jessica N. McAlpine Gregory J. Hannon Georgia Battistoni Dario Bressan Ian G. Cannell Hannah Casbolt Cristina Jauset Tatjana Kovačević Claire M. Mulvey Fiona Nugent Marta Ribes Isabella Pearson Fatime Qosaj Kirsty Sawicka Sophia A. Wild Elena Williams Emma Laks Austin Smith Daniel Lai Andrew Roth Shankar Balasubramanian Maximilian Lee Bernd Bodenmiller Marcel Burger Laura Kuett Sandra Tietscher Jonas Windhager Edward S. Boyden Shahar Alon Yi Cui Amauche Emenari Daniel Goodwin Emmanouil D. Karagiannis Anubhav Sinha Asmamaw T. Wassie Carlos Caldas Alejandra Bruna Maurizio Callari Wendy Greenwood Giulia Lerda Yaniv Eyal-Lubling Oscar M. Rueda Abigail Shea Owen Harris Robby Becker Flaminia Grimaldo Suvi Harris Sara Lisa Vogl Johanna A. Joyce Spencer S. Watson

How cell-to-cell copy number alterations that underpin genomic instability

10.1038/s41586-022-05249-0 article EN cc-by Nature 2022-10-26

Coral reefs are colored by eukaryotic chromoproteins (CPs) that homologous to green fluorescent protein. CPs differ from proteins (FPs) intensely absorbing visible light give strong colors in ambient light. This endows with certain advantages over FPs, such as instrument-free detection uncomplicated ultra-violet damage or background fluorescence, efficient Förster resonance energy transfer (FRET) quenching, and photoacoustic imaging. Thus, have found utility genetic markers teaching,...

10.1186/s13036-018-0100-0 article EN cc-by Journal of Biological Engineering 2018-05-10
Dimitra Georgopoulou Maurizio Callari Oscar M. Rueda Abigail Shea Alistair Martin and 95 more Agnese Giovannetti Fatime Qosaj A. Dariush Suet‐Feung Chin Larissa S. Carnevalli Elena Provenzano Wendy Greenwood Giulia Lerda Elham Esmaeilishirazifard Martin O’Reilly Violeta Serra Dario Bressan H. Raza Ali M. Al Sa’d Shahar Alon Samuel Aparício Giorgia Battistoni Shankar Balasubramanian Robert O. Becker Bernd Bodenmiller E. S. Boyden Dario Bressan Alejandra Bruna Marcel Burger Carlos Caldas Maurizio Callari Ian G. Cannell Helen Casbolt N. Chornay Yi Cui A. Dariush K. Dinh A. Emenari Y. Eyal-Lubling Jean Fan Ali Fatemi Edward A. Fisher E. A. González-Solares C. Gónzalez-Fernández Douglas C. Goodwin Wendy Greenwood Francesco Grimaldi Gregory J. Hannon Owen Harris Shelley Harris Cristina Jauset Johanna A. Joyce Emmanouil D. Karagiannis Tatjana Kovačević Laura Kuett Russell Kunes Yoldaş A. Küpcü Daniel Lai Emma Laks Hsuan Lee M. Lee Giulia Lerda Y. Li Andrew McPherson Neal L. Millar Claire M. Mulvey Fiona Nugent Ciara H. O’Flanagan Marta Pàez‐Ribes I. Pearsall Fatime Qosaj Andrew Roth Oscar M. Rueda Tamara Ruiz Kirsty Sawicka Leonardo A. Sepúlveda Sohrab P. Shah Abigail Shea Anubhav Sinha Adrian L. Smith S. Tavaré Sandra Tietscher Ignacio Vázquez-Garćıa Siegfried Vogl N. A. Walton Asmamaw T. Wassie Spencer S. Watson Joanna Weselak Sonja Wild Elena Williams Jonas Windhager Tristan Whitmarsh C. Xia Ping Zheng Xiaowei Zhuang Gordon B. Mills H. Raza Ali Sabina S. Cosulich Gregory J. Hannon Alejandra Bruna

The heterogeneity of breast cancer plays a major role in drug response and resistance has been extensively characterized at the genomic level. Here, single-cell mass cytometry (BCMC) panel is optimized to identify cell phenotypes their oncogenic signalling states biobank patient-derived tumour xenograft (PDTX) models representing diversity human cancer. BCMC identifies 13 cellular (11 2 murine), associated with both subtypes specific features. Pre-treatment phenotypic composition determinant...

10.1038/s41467-021-22303-z article EN cc-by Nature Communications 2021-03-31

Abstract Breast cancer is the most commonly diagnosed in women, with distant metastasis being main cause of breast cancer-related deaths. Elucidating changes tumor and immune ecosystems that are associated metastatic disease essential to improve understanding ultimately treatment metastasis. Here, we developed an in-depth, spatially resolved single-cell atlas phenotypic diversity cells primary human tumors matched metastases, using imaging mass cytometry analyze a total 75 unique antibody...

10.1158/0008-5472.can-24-1211 article EN cc-by Cancer Research 2024-10-22

<p>Immune cell phenotypes in PT and metastases. <b>A,</b> Uniform Manifold Approximation Projection for Dimension Reduction (UMAP) visualization of all lymphoid cells from the T-cell panel dataset, colored by type (<i>n</i> = 87 patients). <b>B,</b> Heatmap showing mean expression T-cell–relevant markers across different phenotypic subtypes after graph-based clustering patients; <i>n</i>_cells 91,000). Euclidean distance with average...

10.1158/0008-5472.28122826 preprint EN cc-by 2025-01-02

<p>Overview of the clinical cohort and project workflow. <b>A,</b> Overview experimental The study includes primary breast cancer tissue samples with metastases to four different sites (bone, brain, liver, soft tissue). Three antibody panels were used for IMC on consecutive slides, targeting tumor immune phenotypes. <b>B,</b> Targets three their associated function. <b>C,</b> Proportion indicated cell types per patient in after first graph-based...

10.1158/0008-5472.28122835 preprint EN cc-by 2025-01-02

<p>Phenotypic composition of tumor cells from PT and distant metastasis. <b>A,</b> Left, heatmap showing the median expression level for each cell cluster after flowSOM clustering. Markers used clustering are on <i>x-</i>axis, clusters <i>y-</i>axis. For clustering, all were pooled across samples (<i>n</i> = 87 patients, 559,953 cells), counts in shown (bar plot, gray). Euclidean distance with Ward-D2 linkage was hierarchical rows. Right,...

10.1158/0008-5472.28122832 preprint EN cc-by 2025-01-02

<p>Spatial T and myeloid CNs are shared between primary tissue metastatic sites. <b>A,</b> Relative enrichment of T-cell (top) (bottom) subtypes in tumor vs. stromal patches across the whole cohort (<i>n</i> = 87). <b>B,</b> Heatmaps illustrating cell-type frequencies per (left), (middle), or cell (right) neighborhood. Columns <i>z</i>-scored for visualization purposes. The corresponding bubble diagrams show significant results (FDR <...

10.1158/0008-5472.28122817 preprint EN cc-by 2025-01-02

<p>Phenotypic composition analysis of tumor cells between PT and distant metastasis. <b>A,</b> Flow chart depicting the most common phenotypic cluster in vs. metastatic sample. Stacked bar charts show frequency each across patient cohort lines connect matched samples. <b>B,</b> Compositional similarity samples displayed using PCA on per-patient cell abundances as a proportion out all epithelial (abundances were transformed with centered log-ratio...

10.1158/0008-5472.28122829 preprint EN cc-by 2025-01-02

<p>Immune environment in different metastatic sites and the effect of PT on immune cell composition. <b>A</b> <b>B,</b> T-cell (<b>A</b>) myeloid (<b>B</b>) subtype enrichment or metastasis determined via differential abundance testing using paired design grouped by site metastasis. Bar plot shows log<sub>2</sub>-fold changes (<i>n</i> = 76 patients). <b>C,</b> Boxplot compositional similarity between...

10.1158/0008-5472.28122820 preprint EN cc-by 2025-01-02

<p>Comparison of the immune environment in PT and metastatic sites. <b>A,</b> Compositional similarity samples displayed using PCA on per-patient cell abundances (<i>n</i> = 76 patients; after applying centered log-ratio transformation). Each point represents a single patient sample either or (M) site. <b>B,</b> Immune type enrichment metastasis determined via differential abundance testing paired design. Bar plot shows...

10.1158/0008-5472.28122823 preprint EN cc-by 2025-01-02

<div>Abstract<p>Breast cancer is the most commonly diagnosed in women, with distant metastasis being main cause of breast cancer–related deaths. Elucidating changes tumor and immune ecosystems that are associated metastatic disease essential to improve understanding ultimately treatment metastasis. Here, we developed an in-depth, spatially resolved single-cell atlas phenotypic diversity cells primary human tumors matched metastases, using imaging mass cytometry analyze a total 75...

10.1158/0008-5472.c.7606528 preprint EN 2025-01-02

Abstract Cancer-associated fibroblasts (CAFs) are a diverse cell population within the tumour microenvironment, where they have critical effects on evolution and patient prognosis. To define CAF phenotypes, we analysed single-cell RNA sequencing (scRNA-seq) dataset of over 16,000 stromal cells from tumours 14 breast cancer patients, based which defined functionally annotated nine phenotypes one class pericytes. We validated this classification system in four additional types used highly...

10.1101/2022.12.14.520398 preprint EN cc-by-nd bioRxiv (Cold Spring Harbor Laboratory) 2022-12-16
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