A5053669541- Nicotinic Acetylcholine Receptors Study
- Insect and Pesticide Research
- Ion channel regulation and function
- Receptor Mechanisms and Signaling
- Insect-Plant Interactions and Control
- Cholinesterase and Neurodegenerative Diseases
- Neurobiology and Insect Physiology Research
- American Sports and Literature
- Sports, Gender, and Society
- Virology and Viral Diseases
- Insect Resistance and Genetics
- Sport and Mega-Event Impacts
- Neuroscience and Neuropharmacology Research
- Plant and animal studies
- bioluminescence and chemiluminescence research
- Gambling Behavior and Treatments
- Mosquito-borne diseases and control
- Lipid Membrane Structure and Behavior
- vaccines and immunoinformatics approaches
- Cell Image Analysis Techniques
- Virus-based gene therapy research
- Insect Pest Control Strategies
- Single-cell and spatial transcriptomics
- Historical and Literary Studies
- Cancer Genomics and Diagnostics
University College London
2013-2023
University of Cambridge
2021-2023
Institute of Structural and Molecular Biology
2017
Universidad Nacional del Sur
2017
Fundación Ciencias Exactas y Naturales
2017
University of Buenos Aires
2017
Consejo Nacional de Investigaciones Científicas y Técnicas
2017
Birkbeck, University of London
2017
UCL Australia
2001-2012
Institut Pasteur
2008
The aphid Myzus persicae is a globally significant crop pest that has evolved high levels of resistance to almost all classes insecticide. To date, the neonicotinoids, an economically important class insecticides target nicotinic acetylcholine receptors (nAChRs), have remained effective control measure; however, recent reports in M. represent threat long-term efficacy this chemical class. In study, mechanisms underlying neonicotinoid were investigated using biological, biochemical, and...
Neonicotinoids, such as imidacloprid, are nicotinic acetylcholine receptor (nAChR) agonists with potent insecticidal activity. Since its introduction in the early 1990s, imidacloprid has become one of most extensively used insecticides for both crop protection and animal health applications. As other classes insecticides, resistance to neonicotinoids is a significant threat been identified several pest species, including brown planthopper, Nilaparvata lugens , major rice many parts Asia. In...
Neuropathic pain results from damage to the peripheral sensory nervous system, which may have a number of causes. The calcium channel subunit alpha(2)delta-1 is upregulated in dorsal root ganglion (DRG) neurons several animal models neuropathic pain, and this causally related onset allodynia, non-noxious stimulus becomes painful. therapeutic drugs gabapentin pregabalin (PGB), are both alpha(2)delta ligands, antiallodynic effects, but their mechanism action has remained elusive. To...
The co-transcriptional editing of the Newcastle disease virus (NDV) P gene has been studied by sequence analysis cloned viral genomic RNA and mRNA. Evidence obtained for specific insertion non-templated G nucleotides, consequence which is generation three populations gene-derived mRNAs. encode proteins (P, V W) have a common N-terminal region, but utilize different reading frames at their C termini. Paradoxically, NDV edits its mRNA residues in manner similar to Sendai measles viruses (P →...
Octopamine is a neuromodulator that mediates diverse physiological processes in invertebrates. In some insects, such as honeybees and fruit flies, octopamine has been shown to be major stimulator of adenylyl cyclase function associative learning. To identify an receptor mediating this Drosophila , putative biogenic amine receptors were cloned by novel procedure using PCR single-strand conformation polymorphism. One new receptor, mushroom bodies (OAMB), was identified because human cell lines...
Positive allosteric modulators of α7 nicotinic acetylcholine receptors (nAChRs) have attracted considerable interest as potential tools for the treatment neurological and psychiatric disorders such Alzheimer's disease schizophrenia. However, despite therapeutic usefulness these compounds, little is known about their mechanism action. Here, we examined two potentiators nAChRs (PNU-120596 LY-2087101). From studies with a series subunit chimeras, identified transmembrane regions being critical...
Rat hippocampal interneurons express diverse subtypes of functional nicotinic acetylcholine receptors (nAChRs), including alpha7-containing that have properties unlike those expected for homomeric alpha7 nAChRs. We previously reported a strong correlation between expression the and beta2 subunits in individual neurons. To explore whether co-assembly might occur, these were co-expressed Xenopus oocytes heterologously expressed nAChRs characterized by two-electrode voltage clamp. Co-expression...
Abstract High levels of resistance to spinosad, a macrocyclic lactone insecticide, have been reported previously in western flower thrips, Frankliniella occidentalis , an economically important insect pest vegetables, fruit and ornamental crops. We cloned the nicotinic acetylcholine receptor (nAChR) α6 subunit from F. (Foα6) compared nucleotide sequence Foα6 susceptible spinosad‐resistant populations ( MLFOM R1S respectively). A single change has identified Foα6, resulting replacement...
Conventional nicotinic acetylcholine receptor (nAChR) agonists, such as acetylcholine, act at an extracellular “orthosteric” binding site located the interface between two adjacent subunits. Here, we present evidence of potent activation α7 nAChRs via allosteric transmembrane site. Previous studies have identified a series nAChR-positive modulators (PAMs) that lack agonist activity but are able to potentiate responses orthosteric acetylcholine. It has been shown, for example, TQS acts...
Summary: The Imaging and Molecular Annotation of Xenografts Tumors Cancer Grand Challenges team was set up with the objective developing “next generation” pathology cancer research by using a combination single-cell spatial omics tools to produce 3D molecularly annotated maps tumors. Its activities overlapped, in some cases catalyzed, revolution biology that saw new technologies being deployed investigate roles tumor heterogeneity micro-environment. See related article Stratton et al., p. 22...
Recent studies have shown that RIC-3, originally identified in <i>Caenorhabditis elegans</i> as the protein encoded by gene resistance to inhibitors of cholinesterase (<i>ric-3</i>), can enhance functional expression α7 nicotinic acetylcholine receptors (nAChRs). In present study, influence <i>C. and human RIC-3 upon multiple homomeric (α7, α8, α9) heteromeric (α3β2, α3β4, α4β2, α4β4, α9α10) nAChR subtypes has been examined transfected mammalian cells radioligand binding characterization....
Abstract: Expression of the cloned neuronal nicotinic acetylcholine receptor (nAChR) α7 subunit in several cultured mammalian cell lines has revealed that folding, assembly, and subcellular localization this protein are critically dependent upon nature host cell. In all were examined, high levels detected by metabolic labelling immunoprecipitation after transfection with cDNA. contrast, elevated α‐bungarotoxin binding could be only two nine lines. Both these “α7‐permissive” [rat...