Yangning Lu
A5050031234- Genetics, Aging, and Longevity in Model Organisms
- Circadian rhythm and melatonin
- Photoreceptor and optogenetics research
- Health, Environment, Cognitive Aging
- Neuroendocrine regulation and behavior
- Catalysis for Biomass Conversion
- Neural dynamics and brain function
- Delphi Technique in Research
- Plant Molecular Biology Research
- Chemical Synthesis and Reactions
- Polysaccharides and Plant Cell Walls
- Neuroscience and Neural Engineering
- Spaceflight effects on biology
- Plant Reproductive Biology
- Lipid Membrane Structure and Behavior
- Advanced Electron Microscopy Techniques and Applications
- Force Microscopy Techniques and Applications
- Zebrafish Biomedical Research Applications
- Sulfur-Based Synthesis Techniques
- Advanced Memory and Neural Computing
- Single-cell and spatial transcriptomics
- Micro and Nano Robotics
- Molecular Communication and Nanonetworks
- Cancer Genomics and Diagnostics
- Rabbits: Nutrition, Reproduction, Health
McGovern Institute for Brain Research
2024-2025
Massachusetts Institute of Technology
2024-2025
Lunenfeld-Tanenbaum Research Institute
2016-2022
University of Toronto
2016-2022
Mount Sinai Hospital
2016-2022
Canada Research Chairs
2021
Sinai Hospital
2016-2017
Fudan University
2009-2011
Cell- or network-driven oscillators underlie motor rhythmicity. The identity of C. elegans remains unknown. Through cell ablation, electrophysiology, and calcium imaging, we show: (1) forward backward locomotion is driven by different oscillators; (2) the cholinergic excitatory A-class neurons exhibit intrinsic oscillatory activity that sufficient to drive in absence premotor interneurons; (3) UNC-2 P/Q/N high-voltage-activated current underlies A neuron's oscillation; (4) descending...
The combination of microrobotics and optogenetics enables closed-loop locomotion control a living nematode worm.
Summary: The Imaging and Molecular Annotation of Xenografts Tumors Cancer Grand Challenges team was set up with the objective developing “next generation” pathology cancer research by using a combination single-cell spatial omics tools to produce 3D molecularly annotated maps tumors. Its activities overlapped, in some cases catalyzed, revolution biology that saw new technologies being deployed investigate roles tumor heterogeneity micro-environment. See related article Stratton et al., p. 22...
Abstract Lipid membranes are key to the nanoscale compartmentalization of biological systems, but fluorescent visualization them in intact tissues, with precision, is challenging do high labeling density. Here, we report ultrastructural membrane expansion microscopy (umExM), which combines an innovative label and optimized protocol, support dense tissues for visualization. We validate signal-to-background ratio, uniformity continuity, umExM brain slices, supports imaging proteins at a...
Significance A deep understanding of the neural basis motor behaviors must integrate neuromuscular dynamics, mechanosensory feedback, as well global command signals, to predict behavioral dynamics. Here, we report on an integrative approach define circuit logic underlying locomotion in Caenorhabditis elegans . Our combined experimental and computational analyses revealed that ( i ) neurons C. function oscillators; ii descending interneuron inputs proprioceptive coupling between work...
Neuromodulators shape neural circuit dynamics. Combining electron microscopy, genetics, transcriptome profiling, calcium imaging, and optogenetics, we discovered a peptidergic neuron that modulates C. elegans motor The Six/SO-family homeobox transcription factor UNC-39 governs lineage-specific neurogenesis to give rise RID. RID bears the anatomic hallmarks of specialized endocrine neuron: it harbors near-exclusive dense core vesicles cluster periodically along axon, expresses multiple...
Abstract Lipid membranes are key to the nanoscale compartmentalization of biological systems, but fluorescent visualization them in intact tissues, with precision, is challenging do high labeling density. Here, we report ultrastructural membrane expansion microscopy (umExM), which combines a novel label and optimized protocol, support dense tissues for visualization. We validated signal-to-background ratio, uniformity continuity, umExM brain slices, supported imaging proteins at resolution...
<p>IMAXT Consortium Author List</p>
<div>Summary:<p>The Imaging and Molecular Annotation of Xenografts Tumors Cancer Grand Challenges team was set up with the objective developing “next generation” pathology cancer research by using a combination single-cell spatial omics tools to produce 3D molecularly annotated maps tumors. Its activities overlapped, in some cases catalyzed, revolution biology that saw new technologies being deployed investigate roles tumor heterogeneity micro-environment.</p><p><a...
<p>IMAXT Consortium Author List</p>
Straightforward etherification of benzyl alcohols (1) via intermolecular dehydration can be efficiently catalyzed by sodium bisulfite under solvent-free conditions. In the presence 0.3 mol% or 0.6 amount bisulfite, symmetric and unsymmetric ethers are prepared from corresponding in high yields (up to 95%). Etherification benzhydryl is also discussed.
Abstract Central pattern generators are cell‐ or network-driven oscillators that underlie motor rhythmicity. The existence and identity of C. elegans CPGs remain unknown. Through cell ablation, electrophysiology, calcium imaging, we identified for reverse locomotion. We show the cholinergic excitatory class A neurons exhibit intrinsic oscillatory activity, such an activity can drive locomotion without premotor interneurons. Regulation their either through effecting endogenous constituent...
Summary During development, animals can maintain behavioral output even as the underlying circuits structurally remodel. After hatching, C. elegans undergoes substantial motor neuron expansion and synapse re-wiring while animal continuously moves with an undulatory pattern. To understand how circuit transitions from its juvenile to mature configuration without disrupting functional output, we reconstructed by electron microscopy across larval development. We observed: 1) Embryonic neurons...
A primary goal of neuroscience is to understand how nervous systems, or assemblies neural circuits, generate and control behavior. Testing refining our theories would be greatly facilitated if we could reliably simulate an entire system so replicate the brain dynamics in response any stimuli different contexts. More fundamentally, reconstructing modeling a important milestone understanding it, so, simulating itself one goals, indeed dreams, systems neuroscience. To do requires us identify...
Descending signals from the brain play critical roles in controlling and modulating locomotion kinematics. In Caenorhabditis elegans nervous system, descending AVB premotor interneurons exclusively form gap junctions with B-type motor neurons that drive forward locomotion. We combined genetic analysis, optogenetic manipulation, computational modeling to elucidate function of AVB-B during First, we found some generated intrinsic rhythmic activity, constituting distributed central pattern...
Summary In many animals, there is a direct correspondence between the motor patterns that drive locomotion and neuron innervation onto muscle groups. For example, adult C. elegans moves with symmetric alternating dorsal-ventral bending waves arising from input dorsal ventral muscles. contrast to adult, circuit at juvenile larval stage has asymmetric wiring neurons muscles, but still generates adult-like symmetry. We show in circuit, excitatory inhibitory coordinates contraction of muscles...
Abstract Neuromodulators shape neural circuit dynamics. Combining electron microscopy, genetics, transcriptome profiling, calcium imaging, and optogenetics, we discovered a peptidergic neuron that modulates C. elegans motor The Six/SO-family homeobox transcription factor UNC-39 governs lineage-specific neurogenesis to give rise RID. RID bears the anatomic hallmarks of specialized endocrine neuron: it harbors near-exclusive dense core vesicles cluster periodically along axon, expresses...